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Aptamer and PNIPAAm co-conjugated nanoparticles regulate activity of enzyme with different temperature
Yu, Jiemiao1,2,3; Yang, Liangrong2; Liang, Xiangfeng1; Dong, Tingting2,3; Qu, Hongnan1,2,3; Rong, Meng2,3; Liu, Huizhou2,3
2016-10-01
发表期刊TALANTA
ISSN0039-9140
卷号159期号:OCT页码:47-54
摘要

In this paper, we described a temperature responsive nano-system that can regulate activity of enzyme with different temperature. Temperature responsive polymer poly(N-isopropylacrylamide) (PNIPAAm), with low critical solution temperature of 32 degrees C, was synthesized with thiol modification. PNIPAAm and thrombin aptamer were co-functionalized on the surface of gold nanoparticles for effective regulation of thrombin activity with different temperature. On the one hand, we studied the thermal responsive properties of this inhibitor via UV-visible spectroscopy. On the other hand, we investigated the regulation of thrombin activity by this platform with different temperature. The PNIPAAm chains could extend and shrink with different temperature, which suggested that PNIPAAm on the surface of gold nanoparticles could regulate interaction between thrombin and aptamer according to temperature changing. At 25 degrees C, PNIPAAm was hydrophilic extended state, which blocked the interaction between thrombin and aptamer on the surface of gold nanoparticles, therefore thrombin activity had no change. On the contrary, at 37 degrees C, PNIPAAm transformed from hydrophilic extended state to hydrophobic shrank state, allowing the aptamer to capture thrombin, inhibiting the activity of thrombin. More interestingly, this regulation was reverse to normal condition, where 37 degrees C was always the optimum reaction temperature for most of human enzymes. This system we prepared was opposite, which was capable of inhibiting the thrombin activity at 37 degrees C. Furthermore, this was the first report of regulation of thrombin activity using this temperature responsive platform. (C) 2016 Elsevier B.V. All rights reserved.

关键词Temperature Responsive Gold Nanoparticles Aptamer Thrombin Regulation Activity
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
DOI10.1016/j.talanta.2016.05.077
收录类别SCI
语种英语
关键词[WOS]GOLD NANOPARTICLES ; IN-VIVO ; N-ISOPROPYLACRYLAMIDE ; LIQUID-CHROMATOGRAPHY ; THROMBIN DETECTION ; DNA ; INHIBITION ; APTASENSOR ; BIND ; COAGULATION
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
项目资助者National Natural Science Foundation of China(21106162) ; National Key Natural Science Foundation of China(21136009) ; Youth Innovation Promotion Association, CAS(2016043)
WOS记录号WOS:000381322000008
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21450
专题管理及支撑部门
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Yu, Jiemiao,Yang, Liangrong,Liang, Xiangfeng,et al. Aptamer and PNIPAAm co-conjugated nanoparticles regulate activity of enzyme with different temperature[J]. TALANTA,2016,159(OCT):47-54.
APA Yu, Jiemiao.,Yang, Liangrong.,Liang, Xiangfeng.,Dong, Tingting.,Qu, Hongnan.,...&Liu, Huizhou.(2016).Aptamer and PNIPAAm co-conjugated nanoparticles regulate activity of enzyme with different temperature.TALANTA,159(OCT),47-54.
MLA Yu, Jiemiao,et al."Aptamer and PNIPAAm co-conjugated nanoparticles regulate activity of enzyme with different temperature".TALANTA 159.OCT(2016):47-54.
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