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Aptamer and PNIPAAm co-conjugated nanoparticles regulate activity of enzyme with different temperature
Yu, Jiemiao1,2,3; Yang, Liangrong2; Liang, Xiangfeng1; Dong, Tingting2,3; Qu, Hongnan1,2,3; Rong, Meng2,3; Liu, Huizhou2,3
2016-10-01
Source PublicationTALANTA
ISSN0039-9140
Volume159Issue:OCTPages:47-54
Abstract

In this paper, we described a temperature responsive nano-system that can regulate activity of enzyme with different temperature. Temperature responsive polymer poly(N-isopropylacrylamide) (PNIPAAm), with low critical solution temperature of 32 degrees C, was synthesized with thiol modification. PNIPAAm and thrombin aptamer were co-functionalized on the surface of gold nanoparticles for effective regulation of thrombin activity with different temperature. On the one hand, we studied the thermal responsive properties of this inhibitor via UV-visible spectroscopy. On the other hand, we investigated the regulation of thrombin activity by this platform with different temperature. The PNIPAAm chains could extend and shrink with different temperature, which suggested that PNIPAAm on the surface of gold nanoparticles could regulate interaction between thrombin and aptamer according to temperature changing. At 25 degrees C, PNIPAAm was hydrophilic extended state, which blocked the interaction between thrombin and aptamer on the surface of gold nanoparticles, therefore thrombin activity had no change. On the contrary, at 37 degrees C, PNIPAAm transformed from hydrophilic extended state to hydrophobic shrank state, allowing the aptamer to capture thrombin, inhibiting the activity of thrombin. More interestingly, this regulation was reverse to normal condition, where 37 degrees C was always the optimum reaction temperature for most of human enzymes. This system we prepared was opposite, which was capable of inhibiting the thrombin activity at 37 degrees C. Furthermore, this was the first report of regulation of thrombin activity using this temperature responsive platform. (C) 2016 Elsevier B.V. All rights reserved.

KeywordTemperature Responsive Gold Nanoparticles Aptamer Thrombin Regulation Activity
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1016/j.talanta.2016.05.077
Indexed BySCI
Language英语
WOS KeywordGOLD NANOPARTICLES ; IN-VIVO ; N-ISOPROPYLACRYLAMIDE ; LIQUID-CHROMATOGRAPHY ; THROMBIN DETECTION ; DNA ; INHIBITION ; APTASENSOR ; BIND ; COAGULATION
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
Funding OrganizationNational Natural Science Foundation of China(21106162) ; National Key Natural Science Foundation of China(21136009) ; Youth Innovation Promotion Association, CAS(2016043)
WOS IDWOS:000381322000008
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21450
Collection管理及支撑部门
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yu, Jiemiao,Yang, Liangrong,Liang, Xiangfeng,et al. Aptamer and PNIPAAm co-conjugated nanoparticles regulate activity of enzyme with different temperature[J]. TALANTA,2016,159(OCT):47-54.
APA Yu, Jiemiao.,Yang, Liangrong.,Liang, Xiangfeng.,Dong, Tingting.,Qu, Hongnan.,...&Liu, Huizhou.(2016).Aptamer and PNIPAAm co-conjugated nanoparticles regulate activity of enzyme with different temperature.TALANTA,159(OCT),47-54.
MLA Yu, Jiemiao,et al."Aptamer and PNIPAAm co-conjugated nanoparticles regulate activity of enzyme with different temperature".TALANTA 159.OCT(2016):47-54.
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