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Hybrid Cross-Linked Lipase Aggregates with Magnetic Nanoparticles: A Robust and Recyclable Biocatalysis for the Epoxidation of Oleic Acid
Cui, Jiandong2,3; Cui, Lili2; Jia, Shiru3; Su, Zhiguo1; Zhang, Songping1
2016-09-28
Source PublicationJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN0021-8561
Volume64Issue:38Pages:7179-7187
Abstract

Highly stable and easily recyclable-hybrid magnetic cross-linked lipase aggregates (HM-CSL-CLEAs) were prepared by coaggregation of lipase aggregates with nonfunctionalized magnetic nanoparticles and subsequent chemical cross linking with glutaraldehyde. Analysis by SEM and CLSM indicated that the CLEAs were embedded in nanoparticle aggregates instead of covalently immobilized. The resulting HM-CSL-CLEAs exhibited higher thermostability, storage stability, and reusability than standard CLEAs. For example, HM-CSL-CLEAs maintained >60% of their initial activity after 40 min of incubation at 60 degrees C, whereas standard CLEAs lost most of their activities. The HM-CSL-CLEAs can be easily recovered from the reaction mixture by an external magnetic field. Moreover, the H2O2 tolerance of the lipase in HM-CSL-CLEAs was also enhanced, which could relieve the inhibitory effect on lipase activity. A high conversion yield (55%) for the epoxidation of oleic acid using H2O2 as oxidizing agent was achieved by HM-CSL-CLEAs.

KeywordMagnetic Nanoparticles Cross-linked Enzyme Aggregates Lipase Epoxidation Enzyme Immobilization
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1021/acs.jafc.6b01939
Indexed BySCI
Language英语
WOS KeywordCANDIDA-RUGOSA LIPASE ; BOVINE SERUM-ALBUMIN ; HYDROGEN-PEROXIDE ; BIODIESEL PRODUCTION ; IMMOBILIZED LIPASES ; CLEAS ; SILICA ; PERFORMANCE ; ESTERIFICATION ; ELIMINATION
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
Funding OrganizationNational Natural Science Foundation of China(21676069 ; 973 Program(2013CB733604) ; National Major Scientific Equipment Development Project(2013YQ14040508) ; Natural Science Foundation of Hebei Province, China(B2014208054) ; Key Foundation of Hebei University of Science and Technology(SW5) ; 21376249 ; 21336010)
WOS IDWOS:000384518200016
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21475
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
2.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Res Ctr Fermentat Engn Hebei, Shijiazhuang, Peoples R China
3.Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Minist Educ, Tianjin Econ & Technol Dev Area TEDA, 29,13th Ave, Tianjin 300457, Peoples R China
Recommended Citation
GB/T 7714
Cui, Jiandong,Cui, Lili,Jia, Shiru,et al. Hybrid Cross-Linked Lipase Aggregates with Magnetic Nanoparticles: A Robust and Recyclable Biocatalysis for the Epoxidation of Oleic Acid[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2016,64(38):7179-7187.
APA Cui, Jiandong,Cui, Lili,Jia, Shiru,Su, Zhiguo,&Zhang, Songping.(2016).Hybrid Cross-Linked Lipase Aggregates with Magnetic Nanoparticles: A Robust and Recyclable Biocatalysis for the Epoxidation of Oleic Acid.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,64(38),7179-7187.
MLA Cui, Jiandong,et al."Hybrid Cross-Linked Lipase Aggregates with Magnetic Nanoparticles: A Robust and Recyclable Biocatalysis for the Epoxidation of Oleic Acid".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 64.38(2016):7179-7187.
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