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Fiber optofluidic biosensor for the label-free detection of DNA hybridization and methylation based on an in-line tunable mode coupler
Gao, Ran1; Lu, Dan-Feng1; Cheng, Jin1; Jiang, Yi2; Jiang, Lan3; Xu, Jian-Dong4; Qi, Zhi-Mei1
2016-12-15
发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
卷号86期号:DEC页码:321-329
摘要

An optical fiber optofluidic biosensor for the detection of DNA hybridization and methylation has been proposed and experimentally demonstrated. An in-line fiber Michelson interferometer was formed in the photonic crystal fiber. A micrhole in the collapsed region, which combined the tunable mode coupler and optofluidic channel, was fabricated by using femtosecond laser micromachining. The mode field diameter of the guided light is changed with the refractive index in the optofluidic channel, which results in the tunable coupling ratio. Label-free detections of the DNA hybridization and methylation have been experimentally demonstrated. The probe single stranded DNA (ssDNA) was bound with the surface of the optofluidic channel through the Poly-L-lysine layer, and the hybridization between a short 22-mer probe ssDNA and a complementary target ssDNA was carried out and detected by interrogating the fringe visibility of the reflection spectrum. Then, the DNA methylation was also detected through the binding between the methylated DNA and the 5-methylcytosine (5-mC) monoclonal antibody. The experiments results demonstrate that the limit of detection of 5 nM is achieved, establishing the tunable mode coupler as a sensitive and versatile biosensor. The sensitive optical fiber optofluidic biosensor possesses high specificity and low temperature cross-sensitivity. (C) 2016 Elsevier B.V. All rights reserved.

关键词Tunable Mode Coupler Photonic Crystal Fiber Dna Hybridization Detection Dna Methylation Detection Optofluidic Biosensor
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.bios.2016.06.060
收录类别SCI
语种英语
关键词[WOS]SURFACE ; INTERFEROMETER ; SYSTEM
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS类目Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
项目资助者National Basic Research Program of China(2015CB352100) ; National Natural Science Foundation of China(61377064) ; Natural Science for Youth Foundation(61401432) ; China Postdoctoral Science Foundation(2015M571118) ; Program of Chinese 863 Project(2015AA043504)
WOS记录号WOS:000384853300045
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21612
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Elect, State Key Lab Thansducer Technol, Beijing 100190, Peoples R China
2.Beijing Inst Technol, Sch Optoelect, Beijing 100081, Peoples R China
3.Beijing Inst Technol, Laser Micro Nano Fabricat Lab, Beijing 100081, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714
Gao, Ran,Lu, Dan-Feng,Cheng, Jin,et al. Fiber optofluidic biosensor for the label-free detection of DNA hybridization and methylation based on an in-line tunable mode coupler[J]. BIOSENSORS & BIOELECTRONICS,2016,86(DEC):321-329.
APA Gao, Ran.,Lu, Dan-Feng.,Cheng, Jin.,Jiang, Yi.,Jiang, Lan.,...&Qi, Zhi-Mei.(2016).Fiber optofluidic biosensor for the label-free detection of DNA hybridization and methylation based on an in-line tunable mode coupler.BIOSENSORS & BIOELECTRONICS,86(DEC),321-329.
MLA Gao, Ran,et al."Fiber optofluidic biosensor for the label-free detection of DNA hybridization and methylation based on an in-line tunable mode coupler".BIOSENSORS & BIOELECTRONICS 86.DEC(2016):321-329.
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