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Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea
Loder, Andrew J.1,3; Han, Yejun1,4; Hawkins, Aaron B.1,3; Lian, Hong1,5; Lipscomb, Gina L.2; Schut, Gerrit J.2; Keller, Matthew W.2; Adams, Michael W. W.2; Kelly, Robert M.1
2016-11-01
Source PublicationMETABOLIC ENGINEERING
ISSN1096-7176
Volume38Issue:NOVPages:446-463
Abstract

The 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) cycle fixes CO2 in extremely thermoacidophilic archaea and holds promise for metabolic engineering because of its thermostability and potentially rapid pathway kinetics. A reaction kinetics model was developed to examine the biological and biotechnological attributes of the 3HP/4HB cycle as it operates in Metallosphaera sedula, based on previous information as well as on kinetic parameters determined here for recombinant versions of five of the cycle enzymes (malonyl-CoA/succinyl-CoA reductase, 3-hydroxypropionyl-CoA synthetase, 3-hydroxypropionyl-CoA dehydratase, acryloyl-CoA reductase, and succinic semialdehyde reductase). The model correctly predicted previously observed features of the cycle: the 35-65% split of carbon flux through the acetyl-CoA and succinate branches, the high abundance and relative ratio of acetyl-CoA/propionyl-CoA carboxylase (ACC) and MCR, and the significance of ACC and hydroxybutyryl-CoA synthetase (HBCS) as regulated control points for the cycle. The model was then used to assess metabolic engineering strategies for incorporating CO2 into chemical intermediates and products of biotechnological importance: acetyl-CoA, succinate, and 3-hydroxypropionate.

KeywordCo2 Fixation 3-hydroxypropionate 4-hydroxybutyrate Metallosphaera Sedula
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.ymben.2016.10.009
Indexed BySCI
Language英语
WOS KeywordAUTOTROPHIC CARBON FIXATION ; METALLOSPHAERA-SEDULA ; ESCHERICHIA-COLI ; 3-HYDROXYPROPIONIC ACID ; CHLOROFLEXUS-AURANTIACUS ; PYROCOCCUS-FURIOSUS ; METAL RESISTANCE ; PATHWAY ; CHEMICALS ; COENZYME
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
Funding OrganizationUS Department of Energy Research ARPA-E Electrofuels Program(DE-AR0000081) ; US National Science Foundation(CBET-1264052 ; US Air Force Office of Scientific Research (AFOSR)(FA9550-13-1-0236) ; US Department of Education GAANN Fellowship(P200A140020) ; NIH Biotechnology Traineeship(2T32GM008776) ; CBET-1264053)
WOS IDWOS:000387984600044
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21663
Collection研究所(批量导入)
Affiliation1.North Carolina State Univ, Dept Chem & Biomol Engn, Raleigh, NC 27695 USA
2.Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
3.Novozymes North Amer Inc, Franklinton, NC 27525 USA
4.Chinese Acad Sci, Inst Proc Engn, 1 Beiertiao, Beijing 100190, Peoples R China
5.Xiamen Univ, Sch Publ Hlth, South Xinag An Rd, Xiamen 361102, Fujan Province, Peoples R China
Recommended Citation
GB/T 7714
Loder, Andrew J.,Han, Yejun,Hawkins, Aaron B.,et al. Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea[J]. METABOLIC ENGINEERING,2016,38(NOV):446-463.
APA Loder, Andrew J..,Han, Yejun.,Hawkins, Aaron B..,Lian, Hong.,Lipscomb, Gina L..,...&Kelly, Robert M..(2016).Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea.METABOLIC ENGINEERING,38(NOV),446-463.
MLA Loder, Andrew J.,et al."Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea".METABOLIC ENGINEERING 38.NOV(2016):446-463.
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