Insight into the impact of two structural calcium ions on the properties of Pleurotus eryngii versatile ligninolytic peroxidase
Gao, Yu1; Zheng, Lanyan1; Li, Jian-Jun2; Du, Yuguang2
2016-12-15
发表期刊ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
ISSN0003-9861
卷号612期号:DEC页码:9-16
摘要

Two structural Ca2+ (proximal and distal) is known to be important for ligninolytic peroxidases. However, few studies toward impact of residues involved in two Ca2+ on properties of ligninolytic peroxidases have been done, especially the proximal one. In this study, mutants of nine residues involved in liganding two Ca2+ of Pleurotus eryngii versatile peroxidase (VP) were investigated. Most mutants almost completely lost activities, except the mutants of proximal Ca2+ - S170A and V192T. In comparison with WT (wild type), optimal pH values of S170A, S170D, and V192T shifted from pH 3.0 to pH 3.5. The order of thermal and pH stabilities of WT, V192T, S170A, and S170D is similar to that of their specific activities: WT > V192T > S170A > S170D. The CD (circular dichroism) results of WT and several mutants indicated that mutations had some effects on secondary structures. For the first time, it was observed that the thermostability of ligninolytic peroxidases is related with proximal Ca2+ too, and the mutant containing distal Ca2+ only was obtained. Our results clearly demonstrated that enzymatic activities, pH and thermal stabilities, Ca2+ content, and secondary structures of VP have close relationship with the residues involved in two structural Ca2+. (C) 2016 Elsevier Inc. All rights reserved.

关键词Versatile Peroxidase Structural Calcium Ion Site-directed Mutagenesis Ph And Thermal Stability Secondary Structure
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.abb.2016.10.004
收录类别SCI
语种英语
关键词[WOS]SITE-DIRECTED MUTAGENESIS ; MANGANESE PEROXIDASE ; LIGNOCELLULOSIC BIOMASS ; FUNGAL PRETREATMENT ; INACTIVATION ; THERMOSTABILITY ; ENVIRONMENT ; STABILITY ; HEME
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
WOS类目Biochemistry & Molecular Biology ; Biophysics
项目资助者National Science Foundation of China(31170765 ; Beijing Natural Science Foundation(5162022) ; 31370799)
WOS记录号WOS:000389163400002
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21833
专题湿法冶金清洁生产技术国家工程实验室
作者单位1.China Med Univ, Dept Microbiol & Parasitol, 77 Puhe Rd, Shenyang 110122, Liaoning, Peoples R China
2.Chinese Acad Sci, Natl Key Lab Biochem Engn, Natl Engn Res Ctr Biotechnol Beijing,Inst Proc En, Key Lab Biopharmaceut Prod & Formulat Engn,PLA, 1 North 2nd St, Beijing 100190, Peoples R China
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Gao, Yu,Zheng, Lanyan,Li, Jian-Jun,et al. Insight into the impact of two structural calcium ions on the properties of Pleurotus eryngii versatile ligninolytic peroxidase[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2016,612(DEC):9-16.
APA Gao, Yu,Zheng, Lanyan,Li, Jian-Jun,&Du, Yuguang.(2016).Insight into the impact of two structural calcium ions on the properties of Pleurotus eryngii versatile ligninolytic peroxidase.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,612(DEC),9-16.
MLA Gao, Yu,et al."Insight into the impact of two structural calcium ions on the properties of Pleurotus eryngii versatile ligninolytic peroxidase".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 612.DEC(2016):9-16.
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