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Improving adjuvanticity of quaternized chitosan-based microgels for H5N1 split vaccine by tailoring the particle properties to achieve antigen dose sparing effect
Wang, Yue-Qi1,2; Fan, Qing-Ze1,2; Liu, Yan1,2; Yue, Hua1; Ma, Xiao-Wei3; Wu, Jie1; Ma, Guang-Hui1,4; Su, Zhi-Guo1
2016-12-30
发表期刊INTERNATIONAL JOURNAL OF PHARMACEUTICS
ISSN0378-5173
卷号515期号:1-2页码:84-93
摘要

In this study, we developed the quaternized chitosan microgels without chemical crosslinking as an adjuvant of H5N1 split vaccine. The microgels with pH-sensitivity, positive surface charge and good biocompatibility, have been demonstrated in favor of enhancing both humoral and cellular immune response. However, the detailed mechanism of the chitosan-based microgels to enhance antigen specific immune responses remains unclear. Therefore, we prepared the quaternized chitosan microgels with well defined quaternization degrees (QDs, 20-80%) and particle sizes (800 nm-5 mu m) by the premix membrane emulsification technique, and investigated the effect of quaternization degree (QD) and size on the adjuvanticity of microgels. Results suggested that microgels with relatively smaller size (807 nm) and moderate quaternization degree (QD 41% and 60%) were favorable for a maximum immune response. The mechanism was studied and explained by examining the characteristics of microgels and investigating the stimulation of bone-marrow derived dendritic cells (BMDCs). Moreover, they induced significantly stronger immune responses at lower antigen doses (known as antigen sparing effect) compared to aluminum adjuvant. These data indicated that a maximum immune response can be achieved by controlling properties of chitosan microgels, which also could serve as a significant guidance for rational design of chitosan-based particle adjuvant. (C) 2016 Elsevier B.V. All rights reserved.

关键词Quaternized Chitosan Microgels Hydrogel Dose Sparing Effect Adjuvant H5n1 Split Vaccine
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.ijpharm.2016.09.082
收录类别SCI
语种英语
关键词[WOS]DENDRITIC CELLS ; DRUG-DELIVERY ; INFLUENZA VACCINES ; IN-VITRO ; VIRUS ; IMMUNOGENICITY ; MICROPARTICLES ; FUTURE ; SAFETY ; ACTIVATION
WOS研究方向Pharmacology & Pharmacy
WOS类目Pharmacology & Pharmacy
项目资助者National Science and Technology Major Project(2014ZX09102045) ; National High Technology Research, Development Program of China(2014AA093604) ; NSFC(21576268)
WOS记录号WOS:000389150700009
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21842
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Hualan Biol Engn Inc, Xinxiang, Henan, Peoples R China
4.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing, Jiangsu, Peoples R China
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Wang, Yue-Qi,Fan, Qing-Ze,Liu, Yan,et al. Improving adjuvanticity of quaternized chitosan-based microgels for H5N1 split vaccine by tailoring the particle properties to achieve antigen dose sparing effect[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2016,515(1-2):84-93.
APA Wang, Yue-Qi.,Fan, Qing-Ze.,Liu, Yan.,Yue, Hua.,Ma, Xiao-Wei.,...&Su, Zhi-Guo.(2016).Improving adjuvanticity of quaternized chitosan-based microgels for H5N1 split vaccine by tailoring the particle properties to achieve antigen dose sparing effect.INTERNATIONAL JOURNAL OF PHARMACEUTICS,515(1-2),84-93.
MLA Wang, Yue-Qi,et al."Improving adjuvanticity of quaternized chitosan-based microgels for H5N1 split vaccine by tailoring the particle properties to achieve antigen dose sparing effect".INTERNATIONAL JOURNAL OF PHARMACEUTICS 515.1-2(2016):84-93.
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