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Improving adjuvanticity of quaternized chitosan-based microgels for H5N1 split vaccine by tailoring the particle properties to achieve antigen dose sparing effect
Wang, Yue-Qi1,2; Fan, Qing-Ze1,2; Liu, Yan1,2; Yue, Hua1; Ma, Xiao-Wei3; Wu, Jie1; Ma, Guang-Hui1,4; Su, Zhi-Guo1
2016-12-30
Source PublicationINTERNATIONAL JOURNAL OF PHARMACEUTICS
ISSN0378-5173
Volume515Issue:1-2Pages:84-93
Abstract

In this study, we developed the quaternized chitosan microgels without chemical crosslinking as an adjuvant of H5N1 split vaccine. The microgels with pH-sensitivity, positive surface charge and good biocompatibility, have been demonstrated in favor of enhancing both humoral and cellular immune response. However, the detailed mechanism of the chitosan-based microgels to enhance antigen specific immune responses remains unclear. Therefore, we prepared the quaternized chitosan microgels with well defined quaternization degrees (QDs, 20-80%) and particle sizes (800 nm-5 mu m) by the premix membrane emulsification technique, and investigated the effect of quaternization degree (QD) and size on the adjuvanticity of microgels. Results suggested that microgels with relatively smaller size (807 nm) and moderate quaternization degree (QD 41% and 60%) were favorable for a maximum immune response. The mechanism was studied and explained by examining the characteristics of microgels and investigating the stimulation of bone-marrow derived dendritic cells (BMDCs). Moreover, they induced significantly stronger immune responses at lower antigen doses (known as antigen sparing effect) compared to aluminum adjuvant. These data indicated that a maximum immune response can be achieved by controlling properties of chitosan microgels, which also could serve as a significant guidance for rational design of chitosan-based particle adjuvant. (C) 2016 Elsevier B.V. All rights reserved.

KeywordQuaternized Chitosan Microgels Hydrogel Dose Sparing Effect Adjuvant H5n1 Split Vaccine
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.ijpharm.2016.09.082
Indexed BySCI
Language英语
WOS KeywordDENDRITIC CELLS ; DRUG-DELIVERY ; INFLUENZA VACCINES ; IN-VITRO ; VIRUS ; IMMUNOGENICITY ; MICROPARTICLES ; FUTURE ; SAFETY ; ACTIVATION
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
Funding OrganizationNational Science and Technology Major Project(2014ZX09102045) ; National High Technology Research, Development Program of China(2014AA093604) ; NSFC(21576268)
WOS IDWOS:000389150700009
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21842
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Hualan Biol Engn Inc, Xinxiang, Henan, Peoples R China
4.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing, Jiangsu, Peoples R China
Recommended Citation
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Wang, Yue-Qi,Fan, Qing-Ze,Liu, Yan,et al. Improving adjuvanticity of quaternized chitosan-based microgels for H5N1 split vaccine by tailoring the particle properties to achieve antigen dose sparing effect[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2016,515(1-2):84-93.
APA Wang, Yue-Qi.,Fan, Qing-Ze.,Liu, Yan.,Yue, Hua.,Ma, Xiao-Wei.,...&Su, Zhi-Guo.(2016).Improving adjuvanticity of quaternized chitosan-based microgels for H5N1 split vaccine by tailoring the particle properties to achieve antigen dose sparing effect.INTERNATIONAL JOURNAL OF PHARMACEUTICS,515(1-2),84-93.
MLA Wang, Yue-Qi,et al."Improving adjuvanticity of quaternized chitosan-based microgels for H5N1 split vaccine by tailoring the particle properties to achieve antigen dose sparing effect".INTERNATIONAL JOURNAL OF PHARMACEUTICS 515.1-2(2016):84-93.
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