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Preliminary characterizations, antioxidant and hepatoprotective activity of polysaccharide from Cistanche deserticola
Guo, Yuanheng1,2; Cao, Lili1; Zhao, Qingsheng1; Zhang, Lijun1,2; Chen, Jinjin1; Liu, Boyan1,2; Zhao, Bing1
2016-12-01
发表期刊INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
卷号93期号:DEC页码:678-685
摘要

To research the preliminary characterizations, antioxidant and hepatoprotective activity of polysaccharides from Cistanche deserticola (CDPs), three polysaccharide fractions, CDP-A, CDP-B and CDP-C, were obtained by successively membrane filtration (microfiltration, ultrafiltration and nanofiltration). Molecular weights, monosaccharide compositions, purities and IR spectra of the three fractions were analyzed. Results showed that CDP-C contained higher proportion of galacturonic acid (GaIUA) than CDP-B and CDP-A. Antioxidant activities were also analyzed and the results revealed that CDP-C possessed the highest activity. Thus, hepatoprotective activity of CDP-C was studied further. In vitro research, CDP-C promoted viability of HepG2 cells. In vivo research, CDP-C ameliorated the alterations induced by alcohol, including serological indexes (alanine transaminase, acid phosphatase, gamma-glutamyl transpeptidase and triglyceride) and hepatic indicators (superoxide dismutase, malondialdehyde, glutathione S-transferase and triglyceride) in model animals. The prominent microvesicular steatosis and mild necrosis in hepatic histopathology of model animals were also attenuated by CDP-C administration. These findings indicated that CDP-C possessed hepatoprotective activity against chronic hepatic injury induced by alcohol. The underlying mechanism might be that CDP-C can reduce the contents of MDA and TG, and modulate the activities of the relative enzyme. This property might associate with GaIUA in CDP-C. (C) 2016 Published by Elsevier B.V.

关键词Cistanche Deserticola Polysaccharides Preliminary Characterizations Antioxidant Hepatoprotective Activity
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.ijbiomac.2016.09.039
收录类别SCI
语种英语
关键词[WOS]ALCOHOLIC LIVER-DISEASE ; IN-VITRO ; INDUCED HEPATOTOXICITY ; STRUCTURAL-CHARACTERIZATION ; PROTECTIVE ACTIVITIES ; HYDROXYL RADICALS ; EXTRACT ; INJURY ; MICE ; PROLIFERATION
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
项目资助者New Technology Research Institute of Alashan (Zhong Ke), Inner Mongolia, China(KFA2013-189) ; National Natural Science Fund of China(21506220)
WOS记录号WOS:000389090900080
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21846
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Div Biorefinery Engn, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Guo, Yuanheng,Cao, Lili,Zhao, Qingsheng,et al. Preliminary characterizations, antioxidant and hepatoprotective activity of polysaccharide from Cistanche deserticola[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,93(DEC):678-685.
APA Guo, Yuanheng.,Cao, Lili.,Zhao, Qingsheng.,Zhang, Lijun.,Chen, Jinjin.,...&Zhao, Bing.(2016).Preliminary characterizations, antioxidant and hepatoprotective activity of polysaccharide from Cistanche deserticola.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,93(DEC),678-685.
MLA Guo, Yuanheng,et al."Preliminary characterizations, antioxidant and hepatoprotective activity of polysaccharide from Cistanche deserticola".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 93.DEC(2016):678-685.
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