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Monolithic biocatalytic systems with enhanced stabilities constructed through biomimetic silicification-induced enzyme immobilization on rGO/FeOOH hydrogel
Yang, Dong1,3; Zhao, Jingjing1,4; Wang, Xueyan1,4; Shi, Jiafu2,3; Zhang, Shaohua1,4; Jiang, Zhongyi1,2,4
2017-01-15
发表期刊BIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
卷号117页码:52-61
摘要In this study, we present a green and facile method of utilizing biomimetic silicification to trigger enzyme immobilization on the surface of the rGO/FeOOH hydrogel for constructing stable monolithic biocatalytic systems. In brief, the rGO/FeOOH hydrogel is firstly prepared through metal ion-induced reduction/assembly of graphene oxide (GO) nanosheets, which is then utilized to adsorb cationic polyethyleneimine (PEI). This cationic PEI, as the mineralization-inducing agent, catalyzes the condensation of silicate to form silica (biomimetic silicification) on the rGO surface, where enzyme is simultaneously entrapped. The resultant rGO/FeOOH/silica hydrogel shows an extraordinary three-dimensional (3D) porous structure. The silica content on the rGO surface can be facilely tailored through changing the silica precursor concentration. Combined with monolithic macroscale of the rGO/FeOOH/silica hydrogel, the acquired monolithic biocatalytic systems display easy recyclability and elevated pH/thermal/recycling/storage stabilities during the catalytic production of 6-aminopenicillanic acid (6-APA) in comparison to enzyme in free form and enzyme adsorbed on rGO/FeOOH hydrogel. Notably, the activity can be retained up to 93.3% of its initial activity after 11 reaction cycles for our biocatalytic systems. (C) 2016 Elsevier B.V. All rights reserved.
关键词Monolithic Biocatalytic Systems Rgo/feooh Hydrogel Biomimetic Silicification Enhanced Stabilities Enzyme Immobilization Penicillin g Acylase
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.bej.2016.11.001
收录类别SCI
语种英语
关键词[WOS]GRAPHENE OXIDE ; RECYCLABLE NANOBIOCATALYST ; HIGH-PERFORMANCE ; NANOPARTICLES ; SILICA ; MINERALIZATION ; COMPOSITES ; AEROGELS ; CARBON ; SHELL
WOS研究方向Biotechnology & Applied Microbiology ; Engineering
WOS类目Biotechnology & Applied Microbiology ; Engineering, Chemical
项目资助者National Natural Science Funds of China(21406163 ; Tianjin Research Program of Application Foundation and Advanced Technology(15JCQNJC10000) ; Open Funding Project of the National Key Laboratory of Biochemical Engineering(2015KF-03) ; Program of Introducing Talents of Discipline to Universities(B06006) ; 91534126 ; 21621004)
WOS记录号WOS:000390968200006
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21868
专题生化工程国家重点实验室
作者单位1.Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Key Lab Green Chem Technol, Tianjin 300072, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Tianjin Univ, Sch Environm Sci & Engn, Tianjin Engn Ctr Biomass Derived Gas & Oil, Tianjin 300072, Peoples R China
4.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China
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GB/T 7714
Yang, Dong,Zhao, Jingjing,Wang, Xueyan,et al. Monolithic biocatalytic systems with enhanced stabilities constructed through biomimetic silicification-induced enzyme immobilization on rGO/FeOOH hydrogel[J]. BIOCHEMICAL ENGINEERING JOURNAL,2017,117:52-61.
APA Yang, Dong,Zhao, Jingjing,Wang, Xueyan,Shi, Jiafu,Zhang, Shaohua,&Jiang, Zhongyi.(2017).Monolithic biocatalytic systems with enhanced stabilities constructed through biomimetic silicification-induced enzyme immobilization on rGO/FeOOH hydrogel.BIOCHEMICAL ENGINEERING JOURNAL,117,52-61.
MLA Yang, Dong,et al."Monolithic biocatalytic systems with enhanced stabilities constructed through biomimetic silicification-induced enzyme immobilization on rGO/FeOOH hydrogel".BIOCHEMICAL ENGINEERING JOURNAL 117(2017):52-61.
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