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Adjuvanticity Regulation by Biodegradable Polymeric Nano/microparticle Size
Jia, Jilei1,2; Zhang, Weifeng1,2; Liu, Qi1,2; Yang, Tingyuan1; Wang, Lianyan1; Ma, Guanghui1,3

Polymeric nano/microparticles as vaccine adjuvants have been researched in experimental and clinical studies. A more profound understanding of how the physicochemical properties regulate specific immune responses has become a vital requirement. Here we prepared poly(D,Llactic-co-glycolic acid) (PLGA) nano/microparticles with uniform sizes (500 nm, 900 nm, 2.1 mu m, and 4.9 mu m), and the size effects on particle uptake, activation of macrophages, and antigen internalization were evaluated. Particle uptake kinetic studies demonstrated that 900 nm particles were the easiest to accumulate in cells. Moreover, they could induce macrophages to secrete NO and IL-1 beta and facilitate antigen internalization. Furthermore, 900 nm particles, mixed with antigen, could exhibit superior adjuvanticity in both humoral and cellular immune responses in vivo, including offering the highest antibody protection, promoting the maximum secretion levels of IFN-gamma and IL-4 than particles with other sizes. Overall, 900 nm might be the optimum choice for PLGA particle-based vaccine adjuvants especially for recombinant antigens. Understanding the effect of particle size on the adjuvanticity based immune responses might have important enlightenments for rational vaccine design and applications.

KeywordPlga Nano/microparticles Particle Size Adjuvanticity Vaccine Delivery
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
WOS KeywordParticulate Vaccine Adjuvants ; Raw 264.7 Cells ; Immune-response ; Particle-size ; In-vitro ; Drug-release ; Dendritic Cells ; Surface-charge ; Microparticles ; Nanoparticles
WOS Research AreaResearch & Experimental Medicine ; Pharmacology & Pharmacy
WOS SubjectMedicine, Research & Experimental ; Pharmacology & Pharmacy
Funding OrganizationNational Science and Technology Major Project of China(2014ZX09102045-003) ; National Science Foundation of China(21476243) ; 973 Program(2013CB531500)
WOS IDWOS:000391249000002
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Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing 210023, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Jia, Jilei,Zhang, Weifeng,Liu, Qi,et al. Adjuvanticity Regulation by Biodegradable Polymeric Nano/microparticle Size[J]. MOLECULAR PHARMACEUTICS,2017,14(1):14-22.
APA Jia, Jilei,Zhang, Weifeng,Liu, Qi,Yang, Tingyuan,Wang, Lianyan,&Ma, Guanghui.(2017).Adjuvanticity Regulation by Biodegradable Polymeric Nano/microparticle Size.MOLECULAR PHARMACEUTICS,14(1),14-22.
MLA Jia, Jilei,et al."Adjuvanticity Regulation by Biodegradable Polymeric Nano/microparticle Size".MOLECULAR PHARMACEUTICS 14.1(2017):14-22.
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