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Enzyme-immobilized clay nanotube-chitosan membranes with sustainable biocatalytic activities
Sun, Jiajia1,2; Yendluri, Raghuvara3,4; Liu, Kai1,5; Guo, Ying2; Lvov, Yuri3,4,6; Yan, Xuehai1

We have developed a simple and effective strategy to prepare an enzymatic membrane by the admixing of a halloysite clay nanotube-lipase complex and a chitosan solution. The loading of 3 wt% lipase into the lumen of halloysite clay nanotubes was enhanced with an electrostatic interaction. 10 wt% of lipase-nanotubes were dispersed in an aqueous chitosan solution and cast as a coating. The enzymatic chitosan-halloysite membranes displayed increased pH, temperature stability and long-term usability for the catalytic decomposition of lipids: 90% of the initial activity was preserved at 80 degrees C, 70% of the functionality remained at the high pH of 9, and 85% of the activity was sustained after ten continuous cycles of use. The placement of the second antimicrobial enzyme-lysozyme at the outer surface of the clay nanotubes allowed for a synergistic increase in the activity of the composite antibacterial formulation. The lipase/lysozyme-co-immobilized clay nanotube-chitosan membranes are promising in anti-biofouling applications.

WOS HeadingsScience & Technology ; Physical Sciences
Indexed BySCI
WOS KeywordHalloysite Nanotubes ; Drug-delivery ; Encapsulation ; Coatings ; Surfaces ; Release ; Chitin ; Films
WOS Research AreaChemistry ; Physics
WOS SubjectChemistry, Physical ; Physics, Atomic, Molecular & Chemical
Funding OrganizationNational Natural Science Foundation of China(21522307 ; Talent Fund of the Recruitment Program of Global Youth Experts ; Chinese Academy of Sciences (CAS)(QYZDB-SSWISC034) ; Russian Science Foundation(14-19-01045) ; 21473208 ; 91434103)
WOS IDWOS:000391725300059
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Harbin Univ Sci & Technol, Sch Chem & Environm Engn, Harbin 150080, Peoples R China
3.Louisiana Tech Univ, Inst Micromfg, Ruston, LA 71270 USA
4.Louisiana Tech Univ, Biomed Engn Program, Ruston, LA 71270 USA
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.I Gubkin Russian State Univ Oil & Gas, Moscow 119991, Russia
Recommended Citation
GB/T 7714
Sun, Jiajia,Yendluri, Raghuvara,Liu, Kai,et al. Enzyme-immobilized clay nanotube-chitosan membranes with sustainable biocatalytic activities[J]. PHYSICAL CHEMISTRY CHEMICAL PHYSICS,2017,19(1):562-567.
APA Sun, Jiajia,Yendluri, Raghuvara,Liu, Kai,Guo, Ying,Lvov, Yuri,&Yan, Xuehai.(2017).Enzyme-immobilized clay nanotube-chitosan membranes with sustainable biocatalytic activities.PHYSICAL CHEMISTRY CHEMICAL PHYSICS,19(1),562-567.
MLA Sun, Jiajia,et al."Enzyme-immobilized clay nanotube-chitosan membranes with sustainable biocatalytic activities".PHYSICAL CHEMISTRY CHEMICAL PHYSICS 19.1(2017):562-567.
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