CAS OpenIR  > 生化工程国家重点实验室
Effect of protein immunogenicity and PEG size and branching on the anti-PEG immune response to PEGylated proteins
Wan, Xue1,2; Zhang, Juankun1; Yu, Weili2; Shen, Lijuan2; Ji, Shaoyang2; Hu, Tao2
2017
发表期刊PROCESS BIOCHEMISTRY
ISSN1359-5113
卷号52页码:183-191
摘要PEGylation has successfully improved the pharmacological properties of therapeutic proteins. However, polyethylene glycol (PEG) has been burdened by immunogenicity that renders a negative clinical effect on therapeutic proteins. The anti-PEG immune response to PEGylated proteins possibly depends on the nature of proteins and the conjugated methoxy PEG (mPEG). Thus, it is necessary to investigate the effects of protein immunogenicity, the extent of PEGylation, the molecular weight (Mw), and the branching of mPEG on the anti-PEG immune response. Ovalbumin, tetanus toxoid cm, TT-TT conjugate, and TT-bovine serum albumin conjugate were used as target proteins. PEGylated proteins with different extents of PEGylation were obtained by fractionation of the PEGylated IT with size exclusion chromatography. The PEGylated proteins with different Mw and branching of mPEG were obtained by modification of TT with linear mPEG (5 kDa and 20 kDa) and branched mPEG (20 kDa). The PEGylated proteins elicited high levels of anti-PEG antibodies (predominantly IgM and IgG1). The anti-PEG immune response depended on the immunogenicity of proteins, the extent of PEGylation, and the Mw of mPEG. In contrast, branching of mPEG had an insignificant effect on the anti-PEG immune response to the PEGylated proteins. (C) 2016 Elsevier Ltd. All rights reserved.
关键词Immunogenicity Pegylation Anti-peg Antibody Conjugation
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.procbio.2016.09.029
收录类别SCI
语种英语
关键词[WOS]GLYCOL-MODIFIED PROTEINS ; POLYETHYLENE-GLYCOL ; ACCELERATED CLEARANCE ; CONJUGATE VACCINE ; IGM ; ANTIBODIES ; LIPOSOMES ; LINKER ; HYPERSENSITIVITY ; PHARMACOKINETICS
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS类目Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
项目资助者Beijing Natural Science Foundation(7142104) ; National Natural Science Foundation of China(20906095 ; STS Project of Chinese Academy of Sciences(KFJ-EW-STS-027 ; 81402861) ; KFJ-EW-STS-098)
WOS记录号WOS:000392774600021
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21892
专题生化工程国家重点实验室
作者单位1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714
Wan, Xue,Zhang, Juankun,Yu, Weili,et al. Effect of protein immunogenicity and PEG size and branching on the anti-PEG immune response to PEGylated proteins[J]. PROCESS BIOCHEMISTRY,2017,52:183-191.
APA Wan, Xue,Zhang, Juankun,Yu, Weili,Shen, Lijuan,Ji, Shaoyang,&Hu, Tao.(2017).Effect of protein immunogenicity and PEG size and branching on the anti-PEG immune response to PEGylated proteins.PROCESS BIOCHEMISTRY,52,183-191.
MLA Wan, Xue,et al."Effect of protein immunogenicity and PEG size and branching on the anti-PEG immune response to PEGylated proteins".PROCESS BIOCHEMISTRY 52(2017):183-191.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wan, Xue]的文章
[Zhang, Juankun]的文章
[Yu, Weili]的文章
百度学术
百度学术中相似的文章
[Wan, Xue]的文章
[Zhang, Juankun]的文章
[Yu, Weili]的文章
必应学术
必应学术中相似的文章
[Wan, Xue]的文章
[Zhang, Juankun]的文章
[Yu, Weili]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。