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Effect of protein immunogenicity and PEG size and branching on the anti-PEG immune response to PEGylated proteins
Wan, Xue1,2; Zhang, Juankun1; Yu, Weili2; Shen, Lijuan2; Ji, Shaoyang2; Hu, Tao2
2017
Source PublicationPROCESS BIOCHEMISTRY
ISSN1359-5113
Volume52Pages:183-191
Abstract

PEGylation has successfully improved the pharmacological properties of therapeutic proteins. However, polyethylene glycol (PEG) has been burdened by immunogenicity that renders a negative clinical effect on therapeutic proteins. The anti-PEG immune response to PEGylated proteins possibly depends on the nature of proteins and the conjugated methoxy PEG (mPEG). Thus, it is necessary to investigate the effects of protein immunogenicity, the extent of PEGylation, the molecular weight (Mw), and the branching of mPEG on the anti-PEG immune response. Ovalbumin, tetanus toxoid cm, TT-TT conjugate, and TT-bovine serum albumin conjugate were used as target proteins. PEGylated proteins with different extents of PEGylation were obtained by fractionation of the PEGylated IT with size exclusion chromatography. The PEGylated proteins with different Mw and branching of mPEG were obtained by modification of TT with linear mPEG (5 kDa and 20 kDa) and branched mPEG (20 kDa). The PEGylated proteins elicited high levels of anti-PEG antibodies (predominantly IgM and IgG1). The anti-PEG immune response depended on the immunogenicity of proteins, the extent of PEGylation, and the Mw of mPEG. In contrast, branching of mPEG had an insignificant effect on the anti-PEG immune response to the PEGylated proteins. (C) 2016 Elsevier Ltd. All rights reserved.

KeywordImmunogenicity Pegylation Anti-peg Antibody Conjugation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.procbio.2016.09.029
Indexed BySCI
Language英语
WOS KeywordGlycol-modified Proteins ; Polyethylene-glycol ; Accelerated Clearance ; Conjugate Vaccine ; Igm ; Antibodies ; Liposomes ; Linker ; Hypersensitivity ; Pharmacokinetics
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
Funding OrganizationBeijing Natural Science Foundation(7142104) ; National Natural Science Foundation of China(20906095 ; STS Project of Chinese Academy of Sciences(KFJ-EW-STS-027 ; 81402861) ; KFJ-EW-STS-098)
WOS IDWOS:000392774600021
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21892
Collection生化工程国家重点实验室
Affiliation1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wan, Xue,Zhang, Juankun,Yu, Weili,et al. Effect of protein immunogenicity and PEG size and branching on the anti-PEG immune response to PEGylated proteins[J]. PROCESS BIOCHEMISTRY,2017,52:183-191.
APA Wan, Xue,Zhang, Juankun,Yu, Weili,Shen, Lijuan,Ji, Shaoyang,&Hu, Tao.(2017).Effect of protein immunogenicity and PEG size and branching on the anti-PEG immune response to PEGylated proteins.PROCESS BIOCHEMISTRY,52,183-191.
MLA Wan, Xue,et al."Effect of protein immunogenicity and PEG size and branching on the anti-PEG immune response to PEGylated proteins".PROCESS BIOCHEMISTRY 52(2017):183-191.
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