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Preparation of Mix-mode Chromatographic Supports Based on Gigaporous Polymer Microspheres
Yu Yuan1; Wu Xing-Lan1; Li Yin1; Huang Yi-Kang1; Jiang Cheng-Wei1; Zhao Fei-Fei1; Wu Jie2; Zhang Rong-Yue1
2016-12-01
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume44Issue:12Pages:1874-1879
Abstract

The mix-mode chromatography supports were prepared, including anion exchange and hydrophobic interaction chromatography. This matrix was based on the gigaporous polymer microspheres from copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate. The microspheres were modified by poly (ethylene imine) and butyl glycidyl ether for mix. mode chromatography. The effects of ion capacity and hydrophobic ligand density on protein capacity and recovery were evaluated. The result indicated that the protein capacity and recovery increased with ion capacity of the supports in the range of 0.2-0.5 mmol/mL. The max capacity was 40 mg/mL and the protein recovery could reach more than 90%. The hydrophobic interaction chromatography occurred when hydrophobic ligand density was more than 0. 03 mmol/mL. The back pressure on these supports was less than 2 MPa at 2000 cm/h. In the same time, the high resolution could be retained even at high flow rate of 2880 cm/h for purification of IgG from human serum. Therefore, this support showed a large potential in high through. put separation.

KeywordMix-mode Chromatography Separation Of Protein Gigaporous Microspheres High Throughput
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.11895/j.issn.0253-3820.160518
Indexed BySCI
Language英语
WOS KeywordEXCHANGE CHROMATOGRAPHY ; PROTEIN CHROMATOGRAPHY ; SEPARATION ; SEPHAROSE ; RESINS ; MEDIA
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000393290300012
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21893
Collection生化工程国家重点实验室
Affiliation1.Beijing Inst Petrochem Technol, Dept Chem Engn, Beijing 102617, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Yu Yuan,Wu Xing-Lan,Li Yin,et al. Preparation of Mix-mode Chromatographic Supports Based on Gigaporous Polymer Microspheres[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2016,44(12):1874-1879.
APA Yu Yuan.,Wu Xing-Lan.,Li Yin.,Huang Yi-Kang.,Jiang Cheng-Wei.,...&Zhang Rong-Yue.(2016).Preparation of Mix-mode Chromatographic Supports Based on Gigaporous Polymer Microspheres.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,44(12),1874-1879.
MLA Yu Yuan,et al."Preparation of Mix-mode Chromatographic Supports Based on Gigaporous Polymer Microspheres".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 44.12(2016):1874-1879.
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