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Chemical Coating Polysaccharide on Gigaporous Polystyrene Microspheres as a High-Speed Protein Chromatography Matrix
Qu, Jian-Bo1; Xu, Yu-Liang1; Liu, Jun-Yi1; Li, Shi-Hai1; Zhou, Wei-Qing2; Liu, Jian-Guo1
2016-12-01
Source PublicationPOLYMER ENGINEERING AND SCIENCE
ISSN0032-3888
Volume56Issue:12Pages:1407-1414
Abstract

Permanent polysaccharide coating on gigaporous polystyrene (PS) microspheres was achieved through direct chemical grafting of dextran via a facile method, minimizing nonspecific adsorption of proteins and providing easily derivatizable groups on microspheres. Chloroacetylated PS microspheres (PS-Cl) were first developed through acetylation, and hydrophilic dextran was directly grafted to hydrophobic PS-Cl via a Williamson reaction (PS-Dex). The homogeneous side-bound dextran coating has the advantage of masking the hydrophobic surface of the PS microspheres, while maintains their original gigaporous structure. After grafting, the hydrophobic surface of the PS microspheres was effectively masked, and nonspecific protein adsorption was greatly reduced. Moreover, the hydroxyl-rich coating can be easily derivatized through classical chemical methods. A column packed with PS-Dex presented both good permeability and mechanical strength at high flow velocities of up to 3612 cm/h. These results implied that PS-Dex should be an ideal matrix for highspeed protein chromatography. (C) 2016 Society of Plastics Engineers.

SubtypeArticle
WOS HeadingsScience & Technology ; Technology ; Physical Sciences
DOI10.1002/pen.24376
Indexed BySCI
Language英语
WOS KeywordPERFUSION CHROMATOGRAPHY ; LIQUID-CHROMATOGRAPHY ; ADSORPTION ; PARTICLES ; SEPARATION ; SUPPORTS ; DEXTRAN ; PHASE
WOS Research AreaEngineering ; Polymer Science
WOS SubjectEngineering, Chemical ; Polymer Science
Funding OrganizationNational Natural Science Foundation of China(21176257 ; Key Science and Technology Program of Qingdao Economic & Technical Development Zone(2013-1-56) ; 21473256 ; 21276280)
WOS IDWOS:000393121400011
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21903
Collection生化工程国家重点实验室
Affiliation1.China Univ Petr East China, Ctr Bioengn & Biotechnol, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Qu, Jian-Bo,Xu, Yu-Liang,Liu, Jun-Yi,et al. Chemical Coating Polysaccharide on Gigaporous Polystyrene Microspheres as a High-Speed Protein Chromatography Matrix[J]. POLYMER ENGINEERING AND SCIENCE,2016,56(12):1407-1414.
APA Qu, Jian-Bo,Xu, Yu-Liang,Liu, Jun-Yi,Li, Shi-Hai,Zhou, Wei-Qing,&Liu, Jian-Guo.(2016).Chemical Coating Polysaccharide on Gigaporous Polystyrene Microspheres as a High-Speed Protein Chromatography Matrix.POLYMER ENGINEERING AND SCIENCE,56(12),1407-1414.
MLA Qu, Jian-Bo,et al."Chemical Coating Polysaccharide on Gigaporous Polystyrene Microspheres as a High-Speed Protein Chromatography Matrix".POLYMER ENGINEERING AND SCIENCE 56.12(2016):1407-1414.
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