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Chemical Coating Polysaccharide on Gigaporous Polystyrene Microspheres as a High-Speed Protein Chromatography Matrix
Qu, Jian-Bo1; Xu, Yu-Liang1; Liu, Jun-Yi1; Li, Shi-Hai1; Zhou, Wei-Qing2; Liu, Jian-Guo1
2016-12-01
发表期刊POLYMER ENGINEERING AND SCIENCE
ISSN0032-3888
卷号56期号:12页码:1407-1414
摘要

Permanent polysaccharide coating on gigaporous polystyrene (PS) microspheres was achieved through direct chemical grafting of dextran via a facile method, minimizing nonspecific adsorption of proteins and providing easily derivatizable groups on microspheres. Chloroacetylated PS microspheres (PS-Cl) were first developed through acetylation, and hydrophilic dextran was directly grafted to hydrophobic PS-Cl via a Williamson reaction (PS-Dex). The homogeneous side-bound dextran coating has the advantage of masking the hydrophobic surface of the PS microspheres, while maintains their original gigaporous structure. After grafting, the hydrophobic surface of the PS microspheres was effectively masked, and nonspecific protein adsorption was greatly reduced. Moreover, the hydroxyl-rich coating can be easily derivatized through classical chemical methods. A column packed with PS-Dex presented both good permeability and mechanical strength at high flow velocities of up to 3612 cm/h. These results implied that PS-Dex should be an ideal matrix for highspeed protein chromatography. (C) 2016 Society of Plastics Engineers.

文章类型Article
WOS标题词Science & Technology ; Technology ; Physical Sciences
DOI10.1002/pen.24376
收录类别SCI
语种英语
关键词[WOS]PERFUSION CHROMATOGRAPHY ; LIQUID-CHROMATOGRAPHY ; ADSORPTION ; PARTICLES ; SEPARATION ; SUPPORTS ; DEXTRAN ; PHASE
WOS研究方向Engineering ; Polymer Science
WOS类目Engineering, Chemical ; Polymer Science
项目资助者National Natural Science Foundation of China(21176257 ; Key Science and Technology Program of Qingdao Economic & Technical Development Zone(2013-1-56) ; 21473256 ; 21276280)
WOS记录号WOS:000393121400011
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21903
专题生化工程国家重点实验室
作者单位1.China Univ Petr East China, Ctr Bioengn & Biotechnol, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714
Qu, Jian-Bo,Xu, Yu-Liang,Liu, Jun-Yi,et al. Chemical Coating Polysaccharide on Gigaporous Polystyrene Microspheres as a High-Speed Protein Chromatography Matrix[J]. POLYMER ENGINEERING AND SCIENCE,2016,56(12):1407-1414.
APA Qu, Jian-Bo,Xu, Yu-Liang,Liu, Jun-Yi,Li, Shi-Hai,Zhou, Wei-Qing,&Liu, Jian-Guo.(2016).Chemical Coating Polysaccharide on Gigaporous Polystyrene Microspheres as a High-Speed Protein Chromatography Matrix.POLYMER ENGINEERING AND SCIENCE,56(12),1407-1414.
MLA Qu, Jian-Bo,et al."Chemical Coating Polysaccharide on Gigaporous Polystyrene Microspheres as a High-Speed Protein Chromatography Matrix".POLYMER ENGINEERING AND SCIENCE 56.12(2016):1407-1414.
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