Facile fabrication of varisized calcium carbonate microspheres as vaccine adjuvants

doi:10.1039/c6tb02845d

CAS OpenIR > 生化工程国家重点实验室

	Facile fabrication of varisized calcium carbonate microspheres as vaccine adjuvants
	Jia, Jilei 1,2; Liu, Qi 1,2; Yang, Tingyuan 1; Wang, Lianyan 1; Ma, Guanghui 1,3
	2017
发表期刊	JOURNAL OF MATERIALS CHEMISTRY B
ISSN	2050-750X
卷号	5 期号:8 页码:1611-1623
摘要	Functional calcium carbonate (CaCO3) particles of micron and submicron sizes used in catalysis and biomedicine have attracted considerable attention for decades. In this paper, the process parameters for CaCO3 crystallization were systematically investigated. Our experimental results demonstrated the significance of temperature during fabrication. Under the optimized conditions, various uniform-sized and spherical CaCO3 microparticles (MPs) with average diameters from 0.8 mm to 5 mm were facilely and rapidly fabricated via different mixing strategies including mechanical stirring, homogenization, and ultrasonication. The physicochemical characteristics of the CaCO3 microspheres were evaluated. And, the hepatitis B surface antigen (HBsAg) used as a model antigen was encapsulated into the particles (1 mm and 4 mm) for investigating the immune responses elicited after vaccination. In vitro, dendritic cells (DCs) were significantly activated by the MP-based vaccine formulations with up-regulated co-stimulatory molecules expression of CD40 and CD83. After immunization, CaCO3 MPs loaded with HBsAg induced greater lymphocyte activation, more cytokine secretion, higher antigen-specific IgG titers and more memory T cell generation to protect against reinfection. Therefore, the CaCO3 MPs, especially the 1 mm particles, could induce strong cellular and humoral immune responses, probably because of easier uptake and more efficient antigen-presentation by DCs. With the advantages of good biocompatibility, high loading capacity and easy preparation, they could be potentially useful as vaccine adjuvants. These results might provide further design principles for potent inorganic particulate adjuvant and delivery systems.
文章类型	Article
WOS标题词	Science & Technology ; Technology
DOI	10.1039/c6tb02845d
收录类别	SCI
语种	英语
关键词[WOS]	DENDRITIC CELLS ; IMMUNE-RESPONSES ; SURFACE-CHARGE ; DRUG-DELIVERY ; PARTICLE-SIZE ; IN-VITRO ; MICROPARTICLES ; NANOPARTICLES ; POLYMER ; CRYSTALLIZATION
WOS研究方向	Materials Science
WOS类目	Materials Science, Biomaterials
项目资助者	973 Program(2013CB531500) ; National Science Foundation of China(21476243) ; National Science and Technology Major Project of China(2014ZX09102045-003)
WOS记录号	WOS:000395921100009
引用统计
文献类型	期刊论文
条目标识符	http://ir.ipe.ac.cn/handle/122111/21996
专题	生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing 210023, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Jia, Jilei,Liu, Qi,Yang, Tingyuan,et al. Facile fabrication of varisized calcium carbonate microspheres as vaccine adjuvants[J]. JOURNAL OF MATERIALS CHEMISTRY B,2017,5(8):1611-1623.
APA	Jia, Jilei,Liu, Qi,Yang, Tingyuan,Wang, Lianyan,&Ma, Guanghui.(2017).Facile fabrication of varisized calcium carbonate microspheres as vaccine adjuvants.JOURNAL OF MATERIALS CHEMISTRY B,5(8),1611-1623.
MLA	Jia, Jilei,et al."Facile fabrication of varisized calcium carbonate microspheres as vaccine adjuvants".JOURNAL OF MATERIALS CHEMISTRY B 5.8(2017):1611-1623.