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Facile fabrication of varisized calcium carbonate microspheres as vaccine adjuvants
Jia, Jilei1,2; Liu, Qi1,2; Yang, Tingyuan1; Wang, Lianyan1; Ma, Guanghui1,3

Functional calcium carbonate (CaCO3) particles of micron and submicron sizes used in catalysis and biomedicine have attracted considerable attention for decades. In this paper, the process parameters for CaCO3 crystallization were systematically investigated. Our experimental results demonstrated the significance of temperature during fabrication. Under the optimized conditions, various uniform-sized and spherical CaCO3 microparticles (MPs) with average diameters from 0.8 mm to 5 mm were facilely and rapidly fabricated via different mixing strategies including mechanical stirring, homogenization, and ultrasonication. The physicochemical characteristics of the CaCO3 microspheres were evaluated. And, the hepatitis B surface antigen (HBsAg) used as a model antigen was encapsulated into the particles (1 mm and 4 mm) for investigating the immune responses elicited after vaccination. In vitro, dendritic cells (DCs) were significantly activated by the MP-based vaccine formulations with up-regulated co-stimulatory molecules expression of CD40 and CD83. After immunization, CaCO3 MPs loaded with HBsAg induced greater lymphocyte activation, more cytokine secretion, higher antigen-specific IgG titers and more memory T cell generation to protect against reinfection. Therefore, the CaCO3 MPs, especially the 1 mm particles, could induce strong cellular and humoral immune responses, probably because of easier uptake and more efficient antigen-presentation by DCs. With the advantages of good biocompatibility, high loading capacity and easy preparation, they could be potentially useful as vaccine adjuvants. These results might provide further design principles for potent inorganic particulate adjuvant and delivery systems.

WOS HeadingsScience & Technology ; Technology
Indexed BySCI
WOS KeywordDendritic Cells ; Immune-responses ; Surface-charge ; Drug-delivery ; Particle-size ; In-vitro ; Microparticles ; Nanoparticles ; Polymer ; Crystallization
WOS Research AreaMaterials Science
WOS SubjectMaterials Science, bioMaterials
Funding Organization973 Program(2013CB531500) ; National Science Foundation of China(21476243) ; National Science and Technology Major Project of China(2014ZX09102045-003)
WOS IDWOS:000395921100009
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Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Inst Proc Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing 210023, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Jia, Jilei,Liu, Qi,Yang, Tingyuan,et al. Facile fabrication of varisized calcium carbonate microspheres as vaccine adjuvants[J]. JOURNAL OF MATERIALS CHEMISTRY B,2017,5(8):1611-1623.
APA Jia, Jilei,Liu, Qi,Yang, Tingyuan,Wang, Lianyan,&Ma, Guanghui.(2017).Facile fabrication of varisized calcium carbonate microspheres as vaccine adjuvants.JOURNAL OF MATERIALS CHEMISTRY B,5(8),1611-1623.
MLA Jia, Jilei,et al."Facile fabrication of varisized calcium carbonate microspheres as vaccine adjuvants".JOURNAL OF MATERIALS CHEMISTRY B 5.8(2017):1611-1623.
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