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A PEGylated bovine hemoglobin as a potent hemoglobin-based oxygen carrier
Wang, Ying1; Wang, Linli1,2; Yu, Weili3; Gao, Dawei2; You, Guoxing1; Li, Penglong1; Zhang, Shan1,2; Zhang, Jun1; Hu, Tao3; Zhao, Lian1; Zhou, Hong1
2017
Source PublicationBIOTECHNOLOGY PROGRESS
ISSN8756-7938
Volume33Issue:1Pages:252-260
Abstract

Hemoglobin (Hb)-based oxygen carriers (HBOCs) have been used as blood substitutes in surgery medicine and oxygen therapeutics for ischemic stroke. As a potent HBOC, the PEGylated Hb has received much attention for its oxygen delivery and plasma expanding ability. Two PEGylated Hbs, Euro-Hb, and MP4 have been developed for clinical trials, using human adult hemoglobin (HbA) as the original substrate. However, HbA was obtained from outdated human blood and its quantity available from this source may not be sufficient for mass production of PEGylated HbA. In contrast, bovine Hb (bHb) has no quantity constraints for its ample resource. Thus, bHb is of potential to function as an alternative substrate to obtain a PEGylated bHb (bHb-PEG). bHb-PEG was prepared under the same reaction condition as HbA-PEG, using maleimide chemistry. The structural, functional, solution and physiological properties of bHb-PEG were determined and compared with those of HbA-PEG. bHb-PEG showed higher hydrodynamic volume, colloidal osmotic pressure, viscosity and P-50 than HbA-PEG. The high P-50 of bHb can partially compensate the PEGylation-induced perturbation in the R to T state transition of HbA. bHb-PEG was non-vasoactive and could efficiently recover the mean arterial pressure of mice suffering from hemorrhagic shock. Thus, bHb-PEG is expected to function as a potent HBOC for its high oxygen delivery and strong plasma expanding ability. (c) 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:252-260, 2017

KeywordBovine Hemoglobin Pegylation Oxygen Affinity Oxygen Carriers
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1002/btpr.2380
Indexed BySCI
Language英语
WOS KeywordPOLYETHYLENE-GLYCOL ; FUNCTIONAL-PROPERTIES ; PEG-HB ; SITES ; MP4 ; DISSOCIATION ; SUBSTITUTES ; CONJUGATE ; DRUGS
WOS Research AreaBiotechnology & Applied Microbiology ; Food Science & Technology
WOS SubjectBiotechnology & Applied Microbiology ; Food Science & Technology
Funding OrganizationNational High Technology Research and Development Program of China(2012AA021902)
WOS IDWOS:000394889400029
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22000
Collection生化工程国家重点实验室
Affiliation1.Acad Mil Med Sci, Inst Transfus Med, Beijing, Peoples R China
2.Yanshan Univ, Dept Biochem Engn, Coll Environm & Chem Engn, Qinhuangdao, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ying,Wang, Linli,Yu, Weili,et al. A PEGylated bovine hemoglobin as a potent hemoglobin-based oxygen carrier[J]. BIOTECHNOLOGY PROGRESS,2017,33(1):252-260.
APA Wang, Ying.,Wang, Linli.,Yu, Weili.,Gao, Dawei.,You, Guoxing.,...&Zhou, Hong.(2017).A PEGylated bovine hemoglobin as a potent hemoglobin-based oxygen carrier.BIOTECHNOLOGY PROGRESS,33(1),252-260.
MLA Wang, Ying,et al."A PEGylated bovine hemoglobin as a potent hemoglobin-based oxygen carrier".BIOTECHNOLOGY PROGRESS 33.1(2017):252-260.
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