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A PEGylated bovine hemoglobin as a potent hemoglobin-based oxygen carrier
Wang, Ying1; Wang, Linli1,2; Yu, Weili3; Gao, Dawei2; You, Guoxing1; Li, Penglong1; Zhang, Shan1,2; Zhang, Jun1; Hu, Tao3; Zhao, Lian1; Zhou, Hong1
2017
发表期刊BIOTECHNOLOGY PROGRESS
ISSN8756-7938
卷号33期号:1页码:252-260
摘要

Hemoglobin (Hb)-based oxygen carriers (HBOCs) have been used as blood substitutes in surgery medicine and oxygen therapeutics for ischemic stroke. As a potent HBOC, the PEGylated Hb has received much attention for its oxygen delivery and plasma expanding ability. Two PEGylated Hbs, Euro-Hb, and MP4 have been developed for clinical trials, using human adult hemoglobin (HbA) as the original substrate. However, HbA was obtained from outdated human blood and its quantity available from this source may not be sufficient for mass production of PEGylated HbA. In contrast, bovine Hb (bHb) has no quantity constraints for its ample resource. Thus, bHb is of potential to function as an alternative substrate to obtain a PEGylated bHb (bHb-PEG). bHb-PEG was prepared under the same reaction condition as HbA-PEG, using maleimide chemistry. The structural, functional, solution and physiological properties of bHb-PEG were determined and compared with those of HbA-PEG. bHb-PEG showed higher hydrodynamic volume, colloidal osmotic pressure, viscosity and P-50 than HbA-PEG. The high P-50 of bHb can partially compensate the PEGylation-induced perturbation in the R to T state transition of HbA. bHb-PEG was non-vasoactive and could efficiently recover the mean arterial pressure of mice suffering from hemorrhagic shock. Thus, bHb-PEG is expected to function as a potent HBOC for its high oxygen delivery and strong plasma expanding ability. (c) 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:252-260, 2017

关键词Bovine Hemoglobin Pegylation Oxygen Affinity Oxygen Carriers
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1002/btpr.2380
收录类别SCI
语种英语
关键词[WOS]POLYETHYLENE-GLYCOL ; FUNCTIONAL-PROPERTIES ; PEG-HB ; SITES ; MP4 ; DISSOCIATION ; SUBSTITUTES ; CONJUGATE ; DRUGS
WOS研究方向Biotechnology & Applied Microbiology ; Food Science & Technology
WOS类目Biotechnology & Applied Microbiology ; Food Science & Technology
项目资助者National High Technology Research and Development Program of China(2012AA021902)
WOS记录号WOS:000394889400029
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22000
专题生化工程国家重点实验室
作者单位1.Acad Mil Med Sci, Inst Transfus Med, Beijing, Peoples R China
2.Yanshan Univ, Dept Biochem Engn, Coll Environm & Chem Engn, Qinhuangdao, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China
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GB/T 7714
Wang, Ying,Wang, Linli,Yu, Weili,et al. A PEGylated bovine hemoglobin as a potent hemoglobin-based oxygen carrier[J]. BIOTECHNOLOGY PROGRESS,2017,33(1):252-260.
APA Wang, Ying.,Wang, Linli.,Yu, Weili.,Gao, Dawei.,You, Guoxing.,...&Zhou, Hong.(2017).A PEGylated bovine hemoglobin as a potent hemoglobin-based oxygen carrier.BIOTECHNOLOGY PROGRESS,33(1),252-260.
MLA Wang, Ying,et al."A PEGylated bovine hemoglobin as a potent hemoglobin-based oxygen carrier".BIOTECHNOLOGY PROGRESS 33.1(2017):252-260.
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