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Construction of pyruvate producing strain with intact pyruvate dehydrogenase and genome-wide transcription analysis
Yang, Maohua1; Zhang, Xiang2
2017-03-01
Source PublicationWORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN0959-3993
Volume33Issue:3
Abstract

To obtain strain YP211 with a high tendency for accumulating pyruvate, central metabolic pathways were modified in Escherichia coli MG1655. Specifically, seven genes (ldhA, pflB, pta-ackA, poxB, ppc, frdBC) were knocked out sequentially and full pyruvate dehydrogenase was retained. In batch fermentation with M9 medium, pyruvate yield and production rate reached 0.63 g/g glucose and 1.89 g/(1 h), respectively. Meanwhile, the production of acetate, succinate, and other carboxylates was effectively controlled. To understand the physiological observations, we further completed genome-wide transcription analysis of wild-type and YP211. As the acetic acid pathways were blocked, the pathways of convertion of pyruvate to phosphoenol pyruvate and acetyl CoA were enhanced. The transcription of pck, as an alternative gene for ppc, was increased by 2.6 times. So even if gene ppc was inactivated, the tricarboxylic acid pathway was still enhanced in YP211. In order to balance intracellular NADH/NAD(+), oxidative phosphorylation and flagellar assembly system were also up-regulated significantly.

KeywordPyruvate Escherichia Coli Transcription Analysis Oxidation-reduction Flagellar Assembly
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s11274-016-2202-5
Indexed BySCI
Language英语
WOS KeywordEscherichia-coli ; Torulopsis-glabrata ; Biotechnological Production ; Glycolytic Flux ; Enhancement ; Growth ; Mutant ; Metabolism ; Expression ; Acid
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
Funding OrganizationNational High Technology Research and Development Program of China(2014AA021900) ; Foundation of Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education(2015IM002) ; Tianjin Key Lab of Industrial Microbiology (Tianjin University of Science Technology)
WOS IDWOS:000398100300018
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22062
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
2.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, 202 North Ind Rd, Jinan 250100, Peoples R China
Recommended Citation
GB/T 7714
Yang, Maohua,Zhang, Xiang. Construction of pyruvate producing strain with intact pyruvate dehydrogenase and genome-wide transcription analysis[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2017,33(3).
APA Yang, Maohua,&Zhang, Xiang.(2017).Construction of pyruvate producing strain with intact pyruvate dehydrogenase and genome-wide transcription analysis.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,33(3).
MLA Yang, Maohua,et al."Construction of pyruvate producing strain with intact pyruvate dehydrogenase and genome-wide transcription analysis".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 33.3(2017).
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