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Construction of pyruvate producing strain with intact pyruvate dehydrogenase and genome-wide transcription analysis
Yang, Maohua1; Zhang, Xiang2
2017-03-01
发表期刊WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN0959-3993
卷号33期号:3
摘要To obtain strain YP211 with a high tendency for accumulating pyruvate, central metabolic pathways were modified in Escherichia coli MG1655. Specifically, seven genes (ldhA, pflB, pta-ackA, poxB, ppc, frdBC) were knocked out sequentially and full pyruvate dehydrogenase was retained. In batch fermentation with M9 medium, pyruvate yield and production rate reached 0.63 g/g glucose and 1.89 g/(1 h), respectively. Meanwhile, the production of acetate, succinate, and other carboxylates was effectively controlled. To understand the physiological observations, we further completed genome-wide transcription analysis of wild-type and YP211. As the acetic acid pathways were blocked, the pathways of convertion of pyruvate to phosphoenol pyruvate and acetyl CoA were enhanced. The transcription of pck, as an alternative gene for ppc, was increased by 2.6 times. So even if gene ppc was inactivated, the tricarboxylic acid pathway was still enhanced in YP211. In order to balance intracellular NADH/NAD(+), oxidative phosphorylation and flagellar assembly system were also up-regulated significantly.
关键词Pyruvate Escherichia Coli Transcription Analysis Oxidation-reduction Flagellar Assembly
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s11274-016-2202-5
收录类别SCI
语种英语
关键词[WOS]ESCHERICHIA-COLI ; TORULOPSIS-GLABRATA ; BIOTECHNOLOGICAL PRODUCTION ; GLYCOLYTIC FLUX ; ENHANCEMENT ; GROWTH ; MUTANT ; METABOLISM ; EXPRESSION ; ACID
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
项目资助者National High Technology Research and Development Program of China(2014AA021900) ; Foundation of Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education(2015IM002) ; Tianjin Key Lab of Industrial Microbiology (Tianjin University of Science Technology)
WOS记录号WOS:000398100300018
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文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22062
专题研究所(批量导入)
作者单位1.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
2.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, 202 North Ind Rd, Jinan 250100, Peoples R China
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Yang, Maohua,Zhang, Xiang. Construction of pyruvate producing strain with intact pyruvate dehydrogenase and genome-wide transcription analysis[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2017,33(3).
APA Yang, Maohua,&Zhang, Xiang.(2017).Construction of pyruvate producing strain with intact pyruvate dehydrogenase and genome-wide transcription analysis.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,33(3).
MLA Yang, Maohua,et al."Construction of pyruvate producing strain with intact pyruvate dehydrogenase and genome-wide transcription analysis".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 33.3(2017).
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