| High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization | |
| Song, Yafeng1,2,3; Fu, Gang1,2; Dong, Huina1,2; Li, Jianjun4,5; Du, Yuguang4,5; Zhang, Dawei1,2 | |
| 2017-03-29 | |
| Source Publication | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| ISSN | 0021-8561 |
| Volume | 65Issue:12Pages:2540-2548 |
| Abstract | The manno endo-1,4-mannosidase (beta-mannanase, EC. 3.2.1.78) catalyzes the random hydrolysis of internal (1 -> 4)-beta-mannosidic linkages in the mannan polymers. A codon optimized beta-mannanase gene from Bacillus licheniformis DSM13 was expressed in Bacillus subtilis. When four Sec-dependent and two Tat-dependent signal peptide sequences cloned from B. subtilis were placed upstream of the target gene, the highest activity of P-mannanase was observed using SPlipA,A as a signal "peptide. Then a 1.25-fold activity beta-mannanase was obtained when another copy of groESL operon was inserted into the genome of host strain. Finally, five different promoters were separately used to enhance the synthesis of the target protein. The results showed that promoter P-mglv a modified maltose-inducible promoter, significantly elevated the production beta-mannanase. After 72 h of flask fermentation, the enzyme activity of P-mannanase in the supernatant when using locust bean gum as substrate reached 2207 U/mL. This work provided a promising beta-mannanase production strain in industrial application. |
| Keyword | Beta-mannanase Bacillus Subtilis Signal Peptides Promoters Chaperones Signal Peptidase |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1021/acsofc.6b05528 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | METHYL PARATHION HYDROLASE ; GRAM-POSITIVE BACTERIA ; EXTRACELLULAR PRODUCTION ; ESCHERICHIA-COLI ; ALPHA-GALACTOSIDASE ; EXPRESSION SYSTEMS ; SIGNAL PEPTIDES ; VECTORS ; TRANSFORMATION ; LICHENIFORMIS |
| WOS Research Area | Agriculture ; Chemistry ; Food Science & Technology |
| WOS Subject | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
| Funding Organization | National Nature Science Foundation of China(31370089 ; State Key Development 973 Program for Basic Research of China(2013CB733600) ; National High Technology Research and Development Program of China (863 Program)(2014AA093511) ; Nature Science Foundation of Tianjin City(16JCYBJC23500 ; Key Projects in the Tianjin Science & Technology Pillar Program(11ZCZDSY08600) ; 21506244) ; 1SJCQNJC09500) |
| WOS ID | WOS:000398247300006 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/22071 |
| Collection | 生化工程国家重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Univ Groningen, Groningen Res Inst Pharm, Dept Chem & Pharmaceut Biol, NL-9713 AV Groningen, Netherlands 4.Natl Engn Res Ctr Biotechnol, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 5.Chinese Acad Sci, Inst Proc Engn, Key Lab Biopharmaceut Prod & Formulat Engn, PLA, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Song, Yafeng,Fu, Gang,Dong, Huina,et al. High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017,65(12):2540-2548. |
| APA | Song, Yafeng,Fu, Gang,Dong, Huina,Li, Jianjun,Du, Yuguang,&Zhang, Dawei.(2017).High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,65(12),2540-2548. |
| MLA | Song, Yafeng,et al."High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.12(2017):2540-2548. |
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