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High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization
Song, Yafeng1,2,3; Fu, Gang1,2; Dong, Huina1,2; Li, Jianjun4,5; Du, Yuguang4,5; Zhang, Dawei1,2
2017-03-29
发表期刊JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN0021-8561
卷号65期号:12页码:2540-2548
摘要The manno endo-1,4-mannosidase (beta-mannanase, EC. 3.2.1.78) catalyzes the random hydrolysis of internal (1 -> 4)-beta-mannosidic linkages in the mannan polymers. A codon optimized beta-mannanase gene from Bacillus licheniformis DSM13 was expressed in Bacillus subtilis. When four Sec-dependent and two Tat-dependent signal peptide sequences cloned from B. subtilis were placed upstream of the target gene, the highest activity of P-mannanase was observed using SPlipA,A as a signal "peptide. Then a 1.25-fold activity beta-mannanase was obtained when another copy of groESL operon was inserted into the genome of host strain. Finally, five different promoters were separately used to enhance the synthesis of the target protein. The results showed that promoter P-mglv a modified maltose-inducible promoter, significantly elevated the production beta-mannanase. After 72 h of flask fermentation, the enzyme activity of P-mannanase in the supernatant when using locust bean gum as substrate reached 2207 U/mL. This work provided a promising beta-mannanase production strain in industrial application.
关键词Beta-mannanase Bacillus Subtilis Signal Peptides Promoters Chaperones Signal Peptidase
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1021/acsofc.6b05528
收录类别SCI
语种英语
关键词[WOS]METHYL PARATHION HYDROLASE ; GRAM-POSITIVE BACTERIA ; EXTRACELLULAR PRODUCTION ; ESCHERICHIA-COLI ; ALPHA-GALACTOSIDASE ; EXPRESSION SYSTEMS ; SIGNAL PEPTIDES ; VECTORS ; TRANSFORMATION ; LICHENIFORMIS
WOS研究方向Agriculture ; Chemistry ; Food Science & Technology
WOS类目Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
项目资助者National Nature Science Foundation of China(31370089 ; State Key Development 973 Program for Basic Research of China(2013CB733600) ; National High Technology Research and Development Program of China (863 Program)(2014AA093511) ; Nature Science Foundation of Tianjin City(16JCYBJC23500 ; Key Projects in the Tianjin Science & Technology Pillar Program(11ZCZDSY08600) ; 21506244) ; 1SJCQNJC09500)
WOS记录号WOS:000398247300006
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22071
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China
3.Univ Groningen, Groningen Res Inst Pharm, Dept Chem & Pharmaceut Biol, NL-9713 AV Groningen, Netherlands
4.Natl Engn Res Ctr Biotechnol, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
5.Chinese Acad Sci, Inst Proc Engn, Key Lab Biopharmaceut Prod & Formulat Engn, PLA, Beijing 100190, Peoples R China
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Song, Yafeng,Fu, Gang,Dong, Huina,et al. High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017,65(12):2540-2548.
APA Song, Yafeng,Fu, Gang,Dong, Huina,Li, Jianjun,Du, Yuguang,&Zhang, Dawei.(2017).High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,65(12),2540-2548.
MLA Song, Yafeng,et al."High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.12(2017):2540-2548.
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