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High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization
Song, Yafeng1,2,3; Fu, Gang1,2; Dong, Huina1,2; Li, Jianjun4,5; Du, Yuguang4,5; Zhang, Dawei1,2
2017-03-29
Source PublicationJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN0021-8561
Volume65Issue:12Pages:2540-2548
AbstractThe manno endo-1,4-mannosidase (beta-mannanase, EC. 3.2.1.78) catalyzes the random hydrolysis of internal (1 -> 4)-beta-mannosidic linkages in the mannan polymers. A codon optimized beta-mannanase gene from Bacillus licheniformis DSM13 was expressed in Bacillus subtilis. When four Sec-dependent and two Tat-dependent signal peptide sequences cloned from B. subtilis were placed upstream of the target gene, the highest activity of P-mannanase was observed using SPlipA,A as a signal "peptide. Then a 1.25-fold activity beta-mannanase was obtained when another copy of groESL operon was inserted into the genome of host strain. Finally, five different promoters were separately used to enhance the synthesis of the target protein. The results showed that promoter P-mglv a modified maltose-inducible promoter, significantly elevated the production beta-mannanase. After 72 h of flask fermentation, the enzyme activity of P-mannanase in the supernatant when using locust bean gum as substrate reached 2207 U/mL. This work provided a promising beta-mannanase production strain in industrial application.
KeywordBeta-mannanase Bacillus Subtilis Signal Peptides Promoters Chaperones Signal Peptidase
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1021/acsofc.6b05528
Indexed BySCI
Language英语
WOS KeywordMETHYL PARATHION HYDROLASE ; GRAM-POSITIVE BACTERIA ; EXTRACELLULAR PRODUCTION ; ESCHERICHIA-COLI ; ALPHA-GALACTOSIDASE ; EXPRESSION SYSTEMS ; SIGNAL PEPTIDES ; VECTORS ; TRANSFORMATION ; LICHENIFORMIS
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
Funding OrganizationNational Nature Science Foundation of China(31370089 ; State Key Development 973 Program for Basic Research of China(2013CB733600) ; National High Technology Research and Development Program of China (863 Program)(2014AA093511) ; Nature Science Foundation of Tianjin City(16JCYBJC23500 ; Key Projects in the Tianjin Science & Technology Pillar Program(11ZCZDSY08600) ; 21506244) ; 1SJCQNJC09500)
WOS IDWOS:000398247300006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22071
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China
3.Univ Groningen, Groningen Res Inst Pharm, Dept Chem & Pharmaceut Biol, NL-9713 AV Groningen, Netherlands
4.Natl Engn Res Ctr Biotechnol, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
5.Chinese Acad Sci, Inst Proc Engn, Key Lab Biopharmaceut Prod & Formulat Engn, PLA, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Song, Yafeng,Fu, Gang,Dong, Huina,et al. High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017,65(12):2540-2548.
APA Song, Yafeng,Fu, Gang,Dong, Huina,Li, Jianjun,Du, Yuguang,&Zhang, Dawei.(2017).High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,65(12),2540-2548.
MLA Song, Yafeng,et al."High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.12(2017):2540-2548.
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