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Facile preparation of salt-tolerant anion-exchange membrane adsorber using hydrophobic membrane as substrate
Fan, Jinxin1,2; Luo, Jianquan1; Chen, Xiangrong1; Wan, Yinhua1
2017-03-25
发表期刊JOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
卷号1490页码:54-62
摘要In this study, a polyvinylidene fluoride (PVDF) hydrophobic membrane with high mechanical property was used as substrate to prepare salt-tolerant anion-exchange (STAE) membrane adsorber. Effective hydrophilization and functionalization of PVDF membrane was realized via polydopamine (PDA) deposition, thus overcoming the drawbacks of hydrophobic substrates including poor water permeability, inert property as well as severe non-specific adsorption. The following polyallylamine (PAH) coupling was carried out at pH 10.0, where unprotonated amine groups on PAH chains were more prone to couple with PDA. This membrane adsorber could remain 75% of protein binding capacity when NaCl concentration increased from 0 to 150 mM, while its protein binding capacity was independent of flow rate from 10 to 100 membrane volume (MV)/min due to its high mechanical strength (tensile strength: 43.58 +/- 2.30 MPa). With 200 mM NaCI addition at pH 7.5, high purity (above 99%) and high recovery (almost 100%) of Immunoglobulin G (IgG) were obtained when using the STAE membrane adsorber to separate IgG/human serum albumin (HSA) mixture, being similar to that without NaCI at pH 6.0 (both under the flow rate of 10-100 MV/min). Finally, the reliable reusability was confirmed by five reuse cycles of protein binding and elution operations. In comparison with commercial membrane adsorber, the new membrane adsorber exhibited a better mechanical property, higher IgG polishing efficiency and reusability, while the protein binding capacity was lower due to less NH2 density on the membrane. The outcome of this work not only offers a facile and effective approach to prepare membrane adsorbers based on hydrophobic membranes, but also demonstrates great potential of this new designed STAE membrane adsorbers for efficient monoclonal antibody (mAb) polishing. (C) 2017 Elsevier B.V. All rights reserved.
关键词Mussel-inspired Salt-tolerant Anion-exchange Membrane Adsorber Monoclonal Antibody Polishing Membrane Chromatography
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2017.02.016
收录类别SCI
语种英语
关键词[WOS]AFFINITY MEMBRANES ; HOLLOW FIBERS ; PURIFICATION ; PROTEIN ; CHROMATOGRAPHY ; POLYDOPAMINE ; COATINGS ; ELECTROSPUN ; CLEARANCE ; PLATFORM
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
项目资助者National High Technology Research and Development Program of China (863 Program)(2012AA021903 ; "100 Talents Program" of "Youth Innovation Promotion Association" Chinese Academy of Sciences(2017069) ; 2014AA021006)
WOS记录号WOS:000397375600008
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22077
专题生化工程国家重点实验室
作者单位1.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, PR, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, PR, Peoples R China
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Fan, Jinxin,Luo, Jianquan,Chen, Xiangrong,et al. Facile preparation of salt-tolerant anion-exchange membrane adsorber using hydrophobic membrane as substrate[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1490:54-62.
APA Fan, Jinxin,Luo, Jianquan,Chen, Xiangrong,&Wan, Yinhua.(2017).Facile preparation of salt-tolerant anion-exchange membrane adsorber using hydrophobic membrane as substrate.JOURNAL OF CHROMATOGRAPHY A,1490,54-62.
MLA Fan, Jinxin,et al."Facile preparation of salt-tolerant anion-exchange membrane adsorber using hydrophobic membrane as substrate".JOURNAL OF CHROMATOGRAPHY A 1490(2017):54-62.
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