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Conjugation of PEG-hexadecane markedly increases the immunogenicity crossMark of pneumococcal polysaccharide conjugate vaccine
Chang, Xin1,2; Yu, Weili2; Ji, Shaoyang2; Shen, Lijuan2; Tan, Aijuan1; Hu, Tao1,2
2017-03-23
Source PublicationVACCINE
ISSN0264-410X
Volume35Issue:13Pages:1698-1704
Abstract

Streptococcus pneumoniae is a serious Gram-positive pathogen that can lead to an invasive pneumococcal disease with high mortality rate. Pneumococcal capsular polysaccharide (PS) is a key virulence determinant and its immunogenicity can be increased by conjugation with a carrier protein. However, the PS-specific cellular and humoral immunity of pneumococcal conjugate vaccine needs further improvement. Hexadecane (HD) is an element of lipid that decorates the surface of nearly all microbial classes. Polyethylene glycol (PEG)-HD conjugate (PEG-HD) is soluble and can act as an adjuvant. In the present study, a novel pneumococcal polysaccharide conjugate vaccine was prepared by conjugation of tetanus toxoid (IT) portion of PS-TT conjugate (PS-TT) with PEG-HD. As compared with PS-TT, conjugation with PEG -HD led to an 8.0-fold increase in the PS-specific IgG titers. Conjugation with PEG -HD also gave rise to 34.9-, 3.6- and 7.7-fold increase in the IFN-gamma, TNF-alpha and IL-5 levels, respectively. Thus, the conjugated PEG -HD has a stimulatory adjuvant activity to potentiate a robust humoral and cellular immunity. Our proposed conjugate was expected to act as an effective pneumococcal conjugate vaccine for prevention of S. pneumoniae infections. (C) 2017 Elsevier Ltd. All rights reserved.

KeywordStreptococcus Pneumoniae Hexadecane Polyethylene Glycol Adjuvant
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.vaccine.2017.02.027
Indexed BySCI
Language英语
WOS KeywordSpacer Arm ; Disease ; Adjuvant ; Generation ; Improves
WOS Research AreaImmunology ; Research & Experimental Medicine
WOS SubjectImmunology ; Medicine, Research & Experimental
Funding OrganizationNational Natural Science Foundation of China(81402861) ; Beijing Natural Science Foundation(7142104) ; Science and Technology Service Network Initiative Project of Chinese Academy of Sciences(KFJEW-STS-027 ; KFJ-EW-STS-098)
WOS IDWOS:000397371600006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22081
Collection生化工程国家重点实验室
Affiliation1.Guizhou Univ, Coll Pharm, Guiyang 550025, Guizhou, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Chang, Xin,Yu, Weili,Ji, Shaoyang,et al. Conjugation of PEG-hexadecane markedly increases the immunogenicity crossMark of pneumococcal polysaccharide conjugate vaccine[J]. VACCINE,2017,35(13):1698-1704.
APA Chang, Xin,Yu, Weili,Ji, Shaoyang,Shen, Lijuan,Tan, Aijuan,&Hu, Tao.(2017).Conjugation of PEG-hexadecane markedly increases the immunogenicity crossMark of pneumococcal polysaccharide conjugate vaccine.VACCINE,35(13),1698-1704.
MLA Chang, Xin,et al."Conjugation of PEG-hexadecane markedly increases the immunogenicity crossMark of pneumococcal polysaccharide conjugate vaccine".VACCINE 35.13(2017):1698-1704.
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