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Conjugation of PEG-hexadecane markedly increases the immunogenicity crossMark of pneumococcal polysaccharide conjugate vaccine
Chang, Xin1,2; Yu, Weili2; Ji, Shaoyang2; Shen, Lijuan2; Tan, Aijuan1; Hu, Tao1,2
2017-03-23
发表期刊VACCINE
ISSN0264-410X
卷号35期号:13页码:1698-1704
摘要Streptococcus pneumoniae is a serious Gram-positive pathogen that can lead to an invasive pneumococcal disease with high mortality rate. Pneumococcal capsular polysaccharide (PS) is a key virulence determinant and its immunogenicity can be increased by conjugation with a carrier protein. However, the PS-specific cellular and humoral immunity of pneumococcal conjugate vaccine needs further improvement. Hexadecane (HD) is an element of lipid that decorates the surface of nearly all microbial classes. Polyethylene glycol (PEG)-HD conjugate (PEG-HD) is soluble and can act as an adjuvant. In the present study, a novel pneumococcal polysaccharide conjugate vaccine was prepared by conjugation of tetanus toxoid (IT) portion of PS-TT conjugate (PS-TT) with PEG-HD. As compared with PS-TT, conjugation with PEG -HD led to an 8.0-fold increase in the PS-specific IgG titers. Conjugation with PEG -HD also gave rise to 34.9-, 3.6- and 7.7-fold increase in the IFN-gamma, TNF-alpha and IL-5 levels, respectively. Thus, the conjugated PEG -HD has a stimulatory adjuvant activity to potentiate a robust humoral and cellular immunity. Our proposed conjugate was expected to act as an effective pneumococcal conjugate vaccine for prevention of S. pneumoniae infections. (C) 2017 Elsevier Ltd. All rights reserved.
关键词Streptococcus Pneumoniae Hexadecane Polyethylene Glycol Adjuvant
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.vaccine.2017.02.027
收录类别SCI
语种英语
关键词[WOS]SPACER ARM ; DISEASE ; ADJUVANT ; GENERATION ; IMPROVES
WOS研究方向Immunology ; Research & Experimental Medicine
WOS类目Immunology ; Medicine, Research & Experimental
项目资助者National Natural Science Foundation of China(81402861) ; Beijing Natural Science Foundation(7142104) ; Science and Technology Service Network Initiative Project of Chinese Academy of Sciences(KFJEW-STS-027 ; KFJ-EW-STS-098)
WOS记录号WOS:000397371600006
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22081
专题生化工程国家重点实验室
作者单位1.Guizhou Univ, Coll Pharm, Guiyang 550025, Guizhou, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
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Chang, Xin,Yu, Weili,Ji, Shaoyang,et al. Conjugation of PEG-hexadecane markedly increases the immunogenicity crossMark of pneumococcal polysaccharide conjugate vaccine[J]. VACCINE,2017,35(13):1698-1704.
APA Chang, Xin,Yu, Weili,Ji, Shaoyang,Shen, Lijuan,Tan, Aijuan,&Hu, Tao.(2017).Conjugation of PEG-hexadecane markedly increases the immunogenicity crossMark of pneumococcal polysaccharide conjugate vaccine.VACCINE,35(13),1698-1704.
MLA Chang, Xin,et al."Conjugation of PEG-hexadecane markedly increases the immunogenicity crossMark of pneumococcal polysaccharide conjugate vaccine".VACCINE 35.13(2017):1698-1704.
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