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Multi-layer dextran-decorated poly(glycidyl methacrylate)-co-divinyl benzene copolymer matrices enabling efficient protein chromatographic separation
Zhang, Kun1,2; Li, Qiang2,4; Fan, Hong3; Li, Suning3; Su, Yue3; Zhao, Lan2; Huang, Yongdong2; Wang, Dan5; Zhang, Zhigang1; Su, Zhiguo2; Ma, Guanghui2,4
2017-03-01
发表期刊REACTIVE & FUNCTIONAL POLYMERS
ISSN1381-5148
卷号112页码:45-52
摘要A strategy to prepare natural polysaccharide-decorated pGMA-DVB by grafting dextran groups was proposed for the development of efficient protein chromatographic separation. Prepared pGMA-DVB microspheres were covalently decorated with dextran through a three-step scheme. Firstly, residual vinyl groups on polymer particles were converted to epoxy groups. Secondly, one layer of dextran was covalently coupled on the microspheres by reacting with epoxy groups in solvent. Thirdly, multi-layer dextran was grafted to microspheres in alkali aqueous solutions. The dextran-decorated microspheres named pGMA-DVB-Dextran were characterized by Fourier transform infrared spectra, scanning electron microscope, atomic force microscopy, laser scanning confocal microscope, and protein adsorption experiment. Consequently, compared to several commercial hydrophilic beads such as Sepharose (R) 4FF and POROS (R) OH, the permeability and hydtophilicity of the modified micro spheres were improved, the contact angle decreased from 153 degrees to 0 degrees, and nonspecific adsorption of proteins was decreased to zero. The covalently drafting amount of dextran onto pGMA-DVB was increased to 241.86 mg/mL microspheres, and the dextran layers were stable after washing with 1 M HCL/NaOH solutions. Functional ligands such as Protein A or DEAE coupled to pGMA-DVB-Dextran can be conveniently Used to separation model protein, IgG or BSA, with >99.5% recovery yield and high dynamic binding capacity, respectively. (c) 2017 Elsevier B.V. All rights reserved.
关键词Hydrophilization Pgma-dvb Microspheres Covalently Decorating Dextran Protein Separation
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Technology
DOI10.1016/jseactfunctpolym.2017.01.003
收录类别SCI
语种英语
关键词[WOS]ION-EXCHANGE CHROMATOGRAPHY ; PRECIPITATION POLYMERIZATION ; PARTICLES ; NANOPARTICLES ; MICROSPHERES ; ADSORPTION ; MEDIA ; FABRICATION ; CAPACITY ; AGAROSE
WOS研究方向Chemistry ; Engineering ; Polymer Science
WOS类目Chemistry, Applied ; Engineering, Chemical ; Polymer Science
项目资助者Natural Science Foundation of China(21206175 ; National Key Technology R&D Program of the Ministry of Science and Technology(2013BAB01B03) ; Doctoral Fund of Hebei Normal University of Science and Technology(2009YB004) ; Natural Science Foundation Project of CQCSTC(cstc2015jcyjA1504) ; National Key Scientific Instrument and Equipment Development Project(2013YQ14040502) ; 21306206 ; 51103158 ; 21476241)
WOS记录号WOS:000394557000005
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22089
专题生化工程国家重点实验室
作者单位1.Hebei Normal Univ Sci & Technol, Qinhuangdao 066004, Hebei, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.China Natl Ctr Biotechnol Dev, Beijing 100039, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Chongqing Univ, Key Lab Chem Proc Clean Energy & Resource Utiliza, Sch Chem & Chem Engn, Dept Chem Engn, Chongqing 400044, Peoples R China
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Zhang, Kun,Li, Qiang,Fan, Hong,et al. Multi-layer dextran-decorated poly(glycidyl methacrylate)-co-divinyl benzene copolymer matrices enabling efficient protein chromatographic separation[J]. REACTIVE & FUNCTIONAL POLYMERS,2017,112:45-52.
APA Zhang, Kun.,Li, Qiang.,Fan, Hong.,Li, Suning.,Su, Yue.,...&Ma, Guanghui.(2017).Multi-layer dextran-decorated poly(glycidyl methacrylate)-co-divinyl benzene copolymer matrices enabling efficient protein chromatographic separation.REACTIVE & FUNCTIONAL POLYMERS,112,45-52.
MLA Zhang, Kun,et al."Multi-layer dextran-decorated poly(glycidyl methacrylate)-co-divinyl benzene copolymer matrices enabling efficient protein chromatographic separation".REACTIVE & FUNCTIONAL POLYMERS 112(2017):45-52.
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