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Multi-layer dextran-decorated poly(glycidyl methacrylate)-co-divinyl benzene copolymer matrices enabling efficient protein chromatographic separation
Zhang, Kun1,2; Li, Qiang2,4; Fan, Hong3; Li, Suning3; Su, Yue3; Zhao, Lan2; Huang, Yongdong2; Wang, Dan5; Zhang, Zhigang1; Su, Zhiguo2; Ma, Guanghui2,4
2017-03-01
Source PublicationREACTIVE & FUNCTIONAL POLYMERS
ISSN1381-5148
Volume112Pages:45-52
Abstract

A strategy to prepare natural polysaccharide-decorated pGMA-DVB by grafting dextran groups was proposed for the development of efficient protein chromatographic separation. Prepared pGMA-DVB microspheres were covalently decorated with dextran through a three-step scheme. Firstly, residual vinyl groups on polymer particles were converted to epoxy groups. Secondly, one layer of dextran was covalently coupled on the microspheres by reacting with epoxy groups in solvent. Thirdly, multi-layer dextran was grafted to microspheres in alkali aqueous solutions. The dextran-decorated microspheres named pGMA-DVB-Dextran were characterized by Fourier transform infrared spectra, scanning electron microscope, atomic force microscopy, laser scanning confocal microscope, and protein adsorption experiment. Consequently, compared to several commercial hydrophilic beads such as Sepharose (R) 4FF and POROS (R) OH, the permeability and hydtophilicity of the modified micro spheres were improved, the contact angle decreased from 153 degrees to 0 degrees, and nonspecific adsorption of proteins was decreased to zero. The covalently drafting amount of dextran onto pGMA-DVB was increased to 241.86 mg/mL microspheres, and the dextran layers were stable after washing with 1 M HCL/NaOH solutions. Functional ligands such as Protein A or DEAE coupled to pGMA-DVB-Dextran can be conveniently Used to separation model protein, IgG or BSA, with >99.5% recovery yield and high dynamic binding capacity, respectively. (c) 2017 Elsevier B.V. All rights reserved.

KeywordHydrophilization Pgma-dvb Microspheres Covalently Decorating Dextran Protein Separation
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
DOI10.1016/jseactfunctpolym.2017.01.003
Indexed BySCI
Language英语
WOS KeywordIon-exchange Chromatography ; Precipitation Polymerization ; Particles ; Nanoparticles ; Microspheres ; Adsorption ; Media ; Fabrication ; Capacity ; Agarose
WOS Research AreaChemistry ; Engineering ; Polymer Science
WOS SubjectChemistry, Applied ; Engineering, Chemical ; Polymer Science
Funding OrganizationNatural Science Foundation of China(21206175 ; National Key Technology R&D Program of the Ministry of Science and Technology(2013BAB01B03) ; Doctoral Fund of Hebei Normal University of Science and Technology(2009YB004) ; Natural Science Foundation Project of CQCSTC(cstc2015jcyjA1504) ; National Key Scientific Instrument and Equipment Development Project(2013YQ14040502) ; 21306206 ; 51103158 ; 21476241)
WOS IDWOS:000394557000005
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22089
Collection生化工程国家重点实验室
Affiliation1.Hebei Normal Univ Sci & Technol, Qinhuangdao 066004, Hebei, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.China Natl Ctr Biotechnol Dev, Beijing 100039, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Chongqing Univ, Key Lab Chem Proc Clean Energy & Resource Utiliza, Sch Chem & Chem Engn, Dept Chem Engn, Chongqing 400044, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Kun,Li, Qiang,Fan, Hong,et al. Multi-layer dextran-decorated poly(glycidyl methacrylate)-co-divinyl benzene copolymer matrices enabling efficient protein chromatographic separation[J]. REACTIVE & FUNCTIONAL POLYMERS,2017,112:45-52.
APA Zhang, Kun.,Li, Qiang.,Fan, Hong.,Li, Suning.,Su, Yue.,...&Ma, Guanghui.(2017).Multi-layer dextran-decorated poly(glycidyl methacrylate)-co-divinyl benzene copolymer matrices enabling efficient protein chromatographic separation.REACTIVE & FUNCTIONAL POLYMERS,112,45-52.
MLA Zhang, Kun,et al."Multi-layer dextran-decorated poly(glycidyl methacrylate)-co-divinyl benzene copolymer matrices enabling efficient protein chromatographic separation".REACTIVE & FUNCTIONAL POLYMERS 112(2017):45-52.
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