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Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship
Wang, Qing1,2; Bao, Luyao1,2; Jia, Chenjun2,3; Li, Mei3; Li, Jian-Jun1,4; Lu, Xuefeng1
2017-03-16
发表期刊BMC BIOTECHNOLOGY
ISSN1472-6750
卷号17
摘要Background: Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k(cat) value below 1 min(-1), which would limit its application in biofuel production. To identify the activity related key residues of cADO is urgently required.
关键词Aldehyde-deformylating Oxygenase Site-directed Mutagenesis Structure-activity Relationship Fatty Alk(A/e) Ne Synechococcus Elongatus Pcc7942 Synechocystis Sp Pcc6803
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1186/s12896-017-0351-8
收录类别SCI
语种英语
关键词[WOS]CYANOBACTERIAL ALDEHYDE ; FATTY ALDEHYDES ; DECARBONYLASE ; CONVERSION ; ALKANES ; INSIGHTS ; ENZYMES ; FORMATE
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
项目资助者National Basic Research Program of China (973)(2011CBA00907 ; National Science Foundation of China(31170765 ; CAS Cross and Cooperation Team for Scientific Innovation(Y31102110A) ; Shandong Natural Science Foundation(JQ201306) ; Shandong Taishan Scholarship ; 2011CBA00902) ; 31370799)
WOS记录号WOS:000396668000001
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22108
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
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GB/T 7714
Wang, Qing,Bao, Luyao,Jia, Chenjun,et al. Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship[J]. BMC BIOTECHNOLOGY,2017,17.
APA Wang, Qing,Bao, Luyao,Jia, Chenjun,Li, Mei,Li, Jian-Jun,&Lu, Xuefeng.(2017).Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship.BMC BIOTECHNOLOGY,17.
MLA Wang, Qing,et al."Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship".BMC BIOTECHNOLOGY 17(2017).
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