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Specific N-glycan alterations are coupled in EMT induced by different density cultivation of MCF 10A epithelial cells
Xu, Qingsong1,2; Niu, Xueming1; Wang, Wenjing3; Yang, Wen1; Du, Yuguang4; Gu, Jianguo1,2; Song, Linsheng1
2017-04-01
发表期刊GLYCOCONJUGATE JOURNAL
ISSN0282-0080
卷号34期号:2页码:219-227
摘要Epithelial-mesenchymal transition (EMT) is a process in tumor progression during which cancer cells undergo dramatic changes acquiring highly invasive properties. In a widespread adoption of TGF-beta-induced EMT model, we have previously observed that expression of bisecting GlcNAc on N-glycans was dramatically decreased. Herein, we performed in vitro studies with the MCF10A cell line. In response to low cell density, MCF10A cells suffered spontaneously morphologic and phenotypic EMT-like changes, including elongated spindle shape, extended out from edge of the cell sheet, cytoskeleton reorganization, vimentin and fibronectin up-regulation, catenins redistribution, and cadherin switching. Moreover, these phenotypic changes were associated with specific N-glycan alterations. Interestingly, the amounts of bisecting GlcNAc structure were declined, by contrast, the formation of beta 1-6 GlcNAc branches were obviously up-regulated during the EMT induced by sparse cell conditions. We further investigated N-glycans on the beta 1-integrin, which is a good target of some glycosyltransferases. The reactivity with E4-PHA lectin decreased, whereas the staining for L4-PHA lectin, which recognizes branched GlcNAc, increased in sparse cell conditions compared with dense cell conditions. Taken together, these results demonstrated that specific N-glycan alterations are coupled in EMT process and promoted cells migration at a low cell density.
关键词Epithelial-mesenchymal Transition Cell Density N-glycan Migration Tumor
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10719-016-9754-3
收录类别SCI
语种英语
关键词[WOS]TO-MESENCHYMAL TRANSITION ; ACETYLGLUCOSAMINYLTRANSFERASE-III ; BREAST-CANCER ; TUMOR MICROENVIRONMENT ; GLYCOSYLATION ; METASTASIS ; MIGRATION ; GROWTH ; SUPPRESSION ; PROGRESSION
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemistry & Molecular Biology
项目资助者National High Technology Research and Development Program of China (863 Program)(2014AA093604) ; Technology Foundation for Selected Overseas Chinese Scholar, Ministry of Personnel of China ; Ocean Public Welfare Scientific Research Project, State Oceanic Administration of China(201405003)
WOS记录号WOS:000396132800009
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22136
专题研究所(批量导入)
作者单位1.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China
2.Tohoku Med & Pharmaceut Univ, Sendai, Miyagi 9818558, Japan
3.Dalian Elite Analyt Instruments Co Ltd, Dalian 116023, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
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Xu, Qingsong,Niu, Xueming,Wang, Wenjing,et al. Specific N-glycan alterations are coupled in EMT induced by different density cultivation of MCF 10A epithelial cells[J]. GLYCOCONJUGATE JOURNAL,2017,34(2):219-227.
APA Xu, Qingsong.,Niu, Xueming.,Wang, Wenjing.,Yang, Wen.,Du, Yuguang.,...&Song, Linsheng.(2017).Specific N-glycan alterations are coupled in EMT induced by different density cultivation of MCF 10A epithelial cells.GLYCOCONJUGATE JOURNAL,34(2),219-227.
MLA Xu, Qingsong,et al."Specific N-glycan alterations are coupled in EMT induced by different density cultivation of MCF 10A epithelial cells".GLYCOCONJUGATE JOURNAL 34.2(2017):219-227.
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