CAS OpenIR  > 研究所(批量导入)
Specific N-glycan alterations are coupled in EMT induced by different density cultivation of MCF 10A epithelial cells
Xu, Qingsong1,2; Niu, Xueming1; Wang, Wenjing3; Yang, Wen1; Du, Yuguang4; Gu, Jianguo1,2; Song, Linsheng1
2017-04-01
Source PublicationGLYCOCONJUGATE JOURNAL
ISSN0282-0080
Volume34Issue:2Pages:219-227
AbstractEpithelial-mesenchymal transition (EMT) is a process in tumor progression during which cancer cells undergo dramatic changes acquiring highly invasive properties. In a widespread adoption of TGF-beta-induced EMT model, we have previously observed that expression of bisecting GlcNAc on N-glycans was dramatically decreased. Herein, we performed in vitro studies with the MCF10A cell line. In response to low cell density, MCF10A cells suffered spontaneously morphologic and phenotypic EMT-like changes, including elongated spindle shape, extended out from edge of the cell sheet, cytoskeleton reorganization, vimentin and fibronectin up-regulation, catenins redistribution, and cadherin switching. Moreover, these phenotypic changes were associated with specific N-glycan alterations. Interestingly, the amounts of bisecting GlcNAc structure were declined, by contrast, the formation of beta 1-6 GlcNAc branches were obviously up-regulated during the EMT induced by sparse cell conditions. We further investigated N-glycans on the beta 1-integrin, which is a good target of some glycosyltransferases. The reactivity with E4-PHA lectin decreased, whereas the staining for L4-PHA lectin, which recognizes branched GlcNAc, increased in sparse cell conditions compared with dense cell conditions. Taken together, these results demonstrated that specific N-glycan alterations are coupled in EMT process and promoted cells migration at a low cell density.
KeywordEpithelial-mesenchymal Transition Cell Density N-glycan Migration Tumor
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10719-016-9754-3
Indexed BySCI
Language英语
WOS KeywordTO-MESENCHYMAL TRANSITION ; ACETYLGLUCOSAMINYLTRANSFERASE-III ; BREAST-CANCER ; TUMOR MICROENVIRONMENT ; GLYCOSYLATION ; METASTASIS ; MIGRATION ; GROWTH ; SUPPRESSION ; PROGRESSION
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
Funding OrganizationNational High Technology Research and Development Program of China (863 Program)(2014AA093604) ; Technology Foundation for Selected Overseas Chinese Scholar, Ministry of Personnel of China ; Ocean Public Welfare Scientific Research Project, State Oceanic Administration of China(201405003)
WOS IDWOS:000396132800009
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22136
Collection研究所(批量导入)
Affiliation1.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China
2.Tohoku Med & Pharmaceut Univ, Sendai, Miyagi 9818558, Japan
3.Dalian Elite Analyt Instruments Co Ltd, Dalian 116023, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Xu, Qingsong,Niu, Xueming,Wang, Wenjing,et al. Specific N-glycan alterations are coupled in EMT induced by different density cultivation of MCF 10A epithelial cells[J]. GLYCOCONJUGATE JOURNAL,2017,34(2):219-227.
APA Xu, Qingsong.,Niu, Xueming.,Wang, Wenjing.,Yang, Wen.,Du, Yuguang.,...&Song, Linsheng.(2017).Specific N-glycan alterations are coupled in EMT induced by different density cultivation of MCF 10A epithelial cells.GLYCOCONJUGATE JOURNAL,34(2),219-227.
MLA Xu, Qingsong,et al."Specific N-glycan alterations are coupled in EMT induced by different density cultivation of MCF 10A epithelial cells".GLYCOCONJUGATE JOURNAL 34.2(2017):219-227.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xu, Qingsong]'s Articles
[Niu, Xueming]'s Articles
[Wang, Wenjing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xu, Qingsong]'s Articles
[Niu, Xueming]'s Articles
[Wang, Wenjing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xu, Qingsong]'s Articles
[Niu, Xueming]'s Articles
[Wang, Wenjing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.