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One-step purification of alpha(1)-antitrypsin by regulating polyelectrolyte ligands on mussel-inspired membrane adsorber
Fan, Jinxin1,2; Luo, Jianquan1,2; Song, Weijie1; Wan, Yinhua1,2
2017-04-15
发表期刊JOURNAL OF MEMBRANE SCIENCE
ISSN0376-7388
卷号528页码:155-162
摘要One-step purification of biomolecules from complex feed is challenging with commercial membrane adsorbers since it is difficult to screen and regulate the ligands on the membrane with traditional techniques for removal of various impurities. By readily grafting cationic polyelectrolyte ligands on a polydopamine (PDA)-coated membrane, a membrane adsorber with suitable ligand (750 kDa polyethylenimine, PEI) was selected to achieve one-step purification of arantitrypsin (AAT) from pretreated human plasma fraction W. It was found that ligand structure and molecular weight showed a significant effect on the properties and separation performance of membrane adsorbers. The attached polyelectrolytes increased membrane hydrophilicity but caused an enhancement of filtration resistance, and quaternization of the ligands (PEI and polyallylamine) further improved the hydrophilicity but decreased the charge quantity. By further screening elution salt concentration, flow rate, buffer pH, loading volume as well as molecular weight of PEI ligands, a high activity recovery of 96.6% and a high purity of 94.6% of AAT were obtained by one-step membrane chromatography, which was even better than those by multiple-step method. This could be owing to the special multilayer structure of PEI chains, which could suppress the impurity protein binding on the adsorption sites by exclusion effect. The present work not only provides new insights into the purification of biomolecules from complex streams with polyelectrolytecoupled membrane adsorbers, but also offers a new approach to design the ligands on membrane adsorber for specific application.
关键词Alpha(1)-arantitrypsin Cationic Polyelectrolyte Polydopamine Anion-exchange Membrane Adsorber Membrane Chromatography
文章类型Article
WOS标题词Science & Technology ; Technology ; Physical Sciences
DOI10.1016/j.memsci.2017.01.037
收录类别SCI
语种英语
关键词[WOS]MIXED MATRIX MEMBRANE ; TRANSFER RADICAL POLYMERIZATION ; ION-EXCHANGE CHROMATOGRAPHY ; HOLLOW-FIBER MEMBRANE ; PROTEIN-BINDING ; HUMAN-PLASMA ; INHIBITOR CONCENTRATE ; FOULING-RESISTANT ; HIGH-CAPACITY ; SEPARATION
WOS研究方向Engineering ; Polymer Science
WOS类目Engineering, Chemical ; Polymer Science
项目资助者National High Technology Research and Development Program of China (863 Program)(2014AA021006 ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017069) ; 2014AA022206)
WOS记录号WOS:000395956400016
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22138
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Fan, Jinxin,Luo, Jianquan,Song, Weijie,et al. One-step purification of alpha(1)-antitrypsin by regulating polyelectrolyte ligands on mussel-inspired membrane adsorber[J]. JOURNAL OF MEMBRANE SCIENCE,2017,528:155-162.
APA Fan, Jinxin,Luo, Jianquan,Song, Weijie,&Wan, Yinhua.(2017).One-step purification of alpha(1)-antitrypsin by regulating polyelectrolyte ligands on mussel-inspired membrane adsorber.JOURNAL OF MEMBRANE SCIENCE,528,155-162.
MLA Fan, Jinxin,et al."One-step purification of alpha(1)-antitrypsin by regulating polyelectrolyte ligands on mussel-inspired membrane adsorber".JOURNAL OF MEMBRANE SCIENCE 528(2017):155-162.
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