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The Novel Property of Heptapeptide of Microcin C7 in Affecting the Cell Growth of Escherichia coli
Ran, Rensen1,2; Zeng, Huan3; Zhao, Dong3; Liu, Ruiyuan1,2; Xu, Xia1
2017-03-01
Source PublicationMOLECULES
ISSN1420-3049
Volume22Issue:3
AbstractMicrocin C7 (McC), widely distributed in enterobacteria, is a promising antibiotic against antibiotic resistance. Previous studies have demonstrated that the heptapeptide of McC is only responsible for recognizing the inner membrane transporter YejABEF to deliver McC into microbial cells, but lacks the capacity for inhibiting microbial cell growth. In this study, the effect of the heptapeptide (MR) and two analogues, N-formylated heptapeptide (f-MR) and N-aceylated heptapeptide (a-MR), on microbial cell growth were examined. It is surprising to find that MR not only inhibits the activity of intracellular -galactosidase, respiratory chain dehydrogenases, and 6-phosphogluconate dehydrogenases (6PGDH), but it is also able to inhibit Escherichia coli growth, and eventually leads to cell death at the lethal concentration of 5.34 mM within 10 min. The modification of MR results in a slight increase in the lethal concentration. Cell membrane integrity at the lethal concentration confirms that MR undergoes the inhibition effect, but not by destroying the cell membrane integrity. The novel property of MR provides a new insight into the Trojan horse strategy of McC and opens a new route for antibiotics design.
KeywordMicrocin C7 Heptapeptide Cell Growth Membrane Integrity
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.3390/molecules22030432
Indexed BySCI
Language英语
WOS KeywordANTIMICROBIAL PEPTIDE ; OUTER-MEMBRANE ; 6-PHOSPHOGLUCONATE DEHYDROGENASE ; INHIBITOR ; ANTIBIOTICS ; PERMEABILITY ; RESISTANCE ; MECHANISM ; ANOPLIN ; ENTEROBACTERIA
WOS Research AreaChemistry
WOS SubjectChemistry, Organic
Funding OrganizationNational Natural Science Foundation of China(91534107 ; 201506230)
WOS IDWOS:000398743500093
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22151
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Sch Chem & Chem Engn, Beijing 100049, Peoples R China
3.Beijing Univ Chem Technol, Coll Chem Engn, Beijing 100029, Peoples R China
Recommended Citation
GB/T 7714
Ran, Rensen,Zeng, Huan,Zhao, Dong,et al. The Novel Property of Heptapeptide of Microcin C7 in Affecting the Cell Growth of Escherichia coli[J]. MOLECULES,2017,22(3).
APA Ran, Rensen,Zeng, Huan,Zhao, Dong,Liu, Ruiyuan,&Xu, Xia.(2017).The Novel Property of Heptapeptide of Microcin C7 in Affecting the Cell Growth of Escherichia coli.MOLECULES,22(3).
MLA Ran, Rensen,et al."The Novel Property of Heptapeptide of Microcin C7 in Affecting the Cell Growth of Escherichia coli".MOLECULES 22.3(2017).
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