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葡聚糖接枝型Protein A介质的层析性能提升
朱凯; 赵岚; 黄永东; 李强; 邱晗; 王启宝; 苏志国; 马光辉
Source Publication过程工程学报
Other Abstract

Sepharose 4FF微球经环氧活化后与葡聚糖溶液反应,得葡聚糖接枝型琼脂糖微球,再经环氧活化和偶联耐碱型Protein A配基,得葡聚糖接枝型高载量Protein A介质,测定了介质在线清洗稳定性能,并进行了热力学研究.结果表明,与常规Protein A介质相比,葡聚糖接枝型Protein A介质的最高流速提高约32%,对抗体h Ig G的动态载量为60.6 mg/m L,分别为常规介质和Mab Select Su Re介质载量的123%和95%;经40次清洗后,葡聚糖接枝型Protein A介质动态载量为原始载量的92%,远高于常规介质的84%,与Mab Select Su Re稳定性...

Keyword抗体纯化 葡聚糖接枝 Protein A层析 载量
Document Type期刊论文
Recommended Citation
GB/T 7714
朱凯,赵岚,黄永东,等. 葡聚糖接枝型Protein A介质的层析性能提升[J]. 过程工程学报,2016(5):"856-861".
APA 朱凯.,赵岚.,黄永东.,李强.,邱晗.,...&马光辉.(2016).葡聚糖接枝型Protein A介质的层析性能提升.过程工程学报(5),"856-861".
MLA 朱凯,et al."葡聚糖接枝型Protein A介质的层析性能提升".过程工程学报 .5(2016):"856-861".
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