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细胞膜分离费氏丙酸杆菌离位转化合成维生素B_(12)
徐国霞; 杜崴; 王自强; 王云山; 张利平; 苏志国
2016
Source Publication过程工程学报
Issue2Pages:"298-302"
Abstract

采用膜分离技术将费氏丙酸杆菌菌体细胞分离,离位合成转化维生素B12,考察了不同类型及孔径的膜对发酵液的处理能力及对菌体细胞活性的损伤情况,分析了不同浓缩条件下维生素B12的转化效果.结果表明,卷式膜比陶瓷膜更适用于发酵液中菌体细胞的分离,用0.22?m卷式膜处理时,细胞的生物活性几乎不受损伤,存活率达99.5%,将发酵液浓缩4倍,中间体Abo-cbi转化率达78.8%,维生素B12产量为53.9 mg/L.

Keyword费氏丙酸杆菌 细胞分离 维生素b12 离位转化
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22403
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
徐国霞,杜崴,王自强,等. 细胞膜分离费氏丙酸杆菌离位转化合成维生素B_(12)[J]. 过程工程学报,2016(2):"298-302".
APA 徐国霞,杜崴,王自强,王云山,张利平,&苏志国.(2016).细胞膜分离费氏丙酸杆菌离位转化合成维生素B_(12).过程工程学报(2),"298-302".
MLA 徐国霞,et al."细胞膜分离费氏丙酸杆菌离位转化合成维生素B_(12)".过程工程学报 .2(2016):"298-302".
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