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混合培养基模式培养杂交瘤细胞表达单克隆抗体
黄亚杰; 朱光凯; 刘珊; 赵永强; 谢波; 刘道军
2016
Source Publication生物学杂志
Issue6Pages:"104-109"
Abstract

采用混合培养基模式将杂交瘤细胞进行减血清悬浮驯化培养,通过优化提高单克隆抗体的表达量,并进行抗体的分离纯化。在杂交瘤细胞悬浮培养优化过程中,最终确定的血清浓度为2%胎牛血清,培养基配比为RPM1640∶SFM4CHO∶Opti CHO(4.5∶4.5∶1),接种密度为3×105cells/m L,采用批次补料流加培养,每2 d流加1次,每次流加量为初始体积的3%,抗体的表达量从原始的84 mg/L提高到168 mg/L。将其进一步在5 L搅拌式生物反应器中进行培养,离心后上清经protein A亲和层析纯化,确定抗体的产量为240 mg/L。

Keyword杂交瘤细胞培养 单克隆抗体 悬浮驯化 亲和层析
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22436
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
黄亚杰,朱光凯,刘珊,等. 混合培养基模式培养杂交瘤细胞表达单克隆抗体[J]. 生物学杂志,2016(6):"104-109".
APA 黄亚杰,朱光凯,刘珊,赵永强,谢波,&刘道军.(2016).混合培养基模式培养杂交瘤细胞表达单克隆抗体.生物学杂志(6),"104-109".
MLA 黄亚杰,et al."混合培养基模式培养杂交瘤细胞表达单克隆抗体".生物学杂志 .6(2016):"104-109".
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