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Regulation of enzyme activity and stability through positional interaction with polyurethane nanofibers.
Ji, Xiaoyuan1,2; Su, Zhiguo1; Liu, Chunxia1; Wang, Ping1,3,4; Zhang, Songping1
2017-05-15
发表期刊BIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
卷号121页码:147-155
摘要Nanofibrous membranes have been adopted as enzyme carriers via surface adsorption, covalent cross linking, and direct electrospinning-embedment showing great advantages and successes over other nanostructured materials, and especially the newly developed hollow nanofibers were proven as an ideal scaffold for through in-situ encapsulation of multiple enzymes during co-axial electrospinning. Here, the coaxial electrospinning-encapsulation method based on hollow nanofiber structures, together with three previous strategies, which are all based on solid-structured nanofibers, were adopted to immobilize a-chymotrypsin (CT). The feasibilities and advantages of hollow nanofibers encapsulated enzyme are fully demonstrated by the catalytic kinetics of the immobilized CT for hydrolysis and transesterification. Compared to a solid nanofibers-based enzyme, the hollow nanofibers encapsulated CT show the highest catalytic efficiency for both hydrolysis and transesterification. Besides, the confinement effect provided by the nano-scaled hollow chamber not only facilitated molecular interactions between enzymes and substrates, but also enhanced the enzymes stability largely. (C) 2017 Elsevier B.V. All rights reserved.
关键词Nano-scale Microenvironment Hollow Nanofiber Co-axial Electrospinning Confinement Effect Transesterification
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.bej.2017.02.007
收录类别SCI
语种英语
关键词[WOS]HOLLOW NANOFIBERS ; POTENTIAL APPLICATIONS ; POLYMER NANOFIBERS ; IMMOBILIZATION ; PERFORMANCE ; BIOSENSORS ; SUPPORTS ; STABILIZATION ; BIOCATALYSIS ; MEMBRANES
WOS研究方向Biotechnology & Applied Microbiology ; Engineering
WOS类目Biotechnology & Applied Microbiology ; Engineering, Chemical
项目资助者National Natural Science Foundation of China(21376249 ; National Basic Research Program of China (973 Program)(2013CB733604) ; National Key Scientific Instrument and Equipment Development Project(2013YQ14040508) ; 21676276)
WOS记录号WOS:000398752600016
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22457
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA
4.Univ Minnesota, Biotechnol Inst, St Paul, MN 55108 USA
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Ji, Xiaoyuan,Su, Zhiguo,Liu, Chunxia,et al. Regulation of enzyme activity and stability through positional interaction with polyurethane nanofibers.[J]. BIOCHEMICAL ENGINEERING JOURNAL,2017,121:147-155.
APA Ji, Xiaoyuan,Su, Zhiguo,Liu, Chunxia,Wang, Ping,&Zhang, Songping.(2017).Regulation of enzyme activity and stability through positional interaction with polyurethane nanofibers..BIOCHEMICAL ENGINEERING JOURNAL,121,147-155.
MLA Ji, Xiaoyuan,et al."Regulation of enzyme activity and stability through positional interaction with polyurethane nanofibers.".BIOCHEMICAL ENGINEERING JOURNAL 121(2017):147-155.
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