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Simultaneous detoxification and preparative separation of chlorogenic acid from Eupatorium adenophorum by combined column chromatography
Liu, Boyan1,2; Dong, Beitao1,2; Yuan, Xiaofan1; Guo, Yuanheng1,2; Zhang, Lijun1,2; Zhao, Bing1
2017
发表期刊SEPARATION SCIENCE AND TECHNOLOGY
ISSN0149-6395
卷号52期号:6页码:1114-1121
摘要An efficient method for simultaneous detoxification and preparative separation of chlorogenic acid from Eupatorium adenophorum was developed. The entire procedure consisted of two separation steps. The first step was enrichment of chlorogenic acid from the crude extract by NKA-II macroporous resin. The purity of chlorogenic acid increased from 5.3% to 20.9% with a recovery of 88.7%. Subsequently, polyamide resin was used to achieve a higher purity. After the polyamide resin treatment, the main toxin 9-oxo-10,11-dehydroageraphorone was removed and the purity of chlorogenic acid reached 86.5% with a recovery of 84.8%. It provided a new way for the utilization of E. adenophorum.
关键词Chlorogenic Acid Eupatorium Adenophorum Spreng Macroporous Resin 9-oxo-10 11-dehydroageraphorone Polyamide Resin
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Technology
DOI10.1080/01496395.2017.1281300
收录类别SCI
语种英语
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; COUNTER-CURRENT CHROMATOGRAPHY ; POLYAMIDE RESIN ; MACROPOROUS RESINS ; AGERATINA-ADENOPHORA ; CAFFEIC ACID ; NATURAL-PRODUCTS ; PURIFICATION ; ADSORPTION ; EXTRACT
WOS研究方向Chemistry ; Engineering
WOS类目Chemistry, Multidisciplinary ; Engineering, Chemical
项目资助者Panzhihua Xiyu Biotech Co., Ltd. ( Sichuan, China)
WOS记录号WOS:000399643200014
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22477
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, Div Biorefinery Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
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Liu, Boyan,Dong, Beitao,Yuan, Xiaofan,et al. Simultaneous detoxification and preparative separation of chlorogenic acid from Eupatorium adenophorum by combined column chromatography[J]. SEPARATION SCIENCE AND TECHNOLOGY,2017,52(6):1114-1121.
APA Liu, Boyan,Dong, Beitao,Yuan, Xiaofan,Guo, Yuanheng,Zhang, Lijun,&Zhao, Bing.(2017).Simultaneous detoxification and preparative separation of chlorogenic acid from Eupatorium adenophorum by combined column chromatography.SEPARATION SCIENCE AND TECHNOLOGY,52(6),1114-1121.
MLA Liu, Boyan,et al."Simultaneous detoxification and preparative separation of chlorogenic acid from Eupatorium adenophorum by combined column chromatography".SEPARATION SCIENCE AND TECHNOLOGY 52.6(2017):1114-1121.
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