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Porous Nanohydroxyapatite/Collagen Scaffolds Loading Insulin PLGA Particles for Restoration of Critical Size Bone Defect
Wang, Xing1,2; Wu, Xia1; Xing, Helin1; Zhang, Guilan1; Shi, Quan1; E, Lingling1; Liu, Na1; Yang, Tingyuan3; Wang, Dongsheng1; Qi, Feng3; Wang, Lianyan3; Liu, Hongchen1
2017-04-05
Source PublicationACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
Volume9Issue:13Pages:11380-11391
AbstractInsulin is considered to be a classical central regulator of energy homeostasis. Recently, the effect of insulin on bone has gained a lot of attention, but little attention has been paid to the application in bone tissue engineering. In this study, porous nanohydroxyapatite/collagen (nHAC) scaffolds incorporating poly lactic-co-glycolic acid (PLGA) particles were successfully developed as an insulin delivery platform for bone regeneration. Bioactive insulin was successfully released from the PLGA particles within the scaffold, and the size of the particles as well as the release kinetics of the insulin could be efficiently controlled through Shirasu porous glass premix membrane emulsification technology. It was indicated that the nHAC/PLGA composite scaffolds possessed favorable mechanical and structural properties for cell adhesion and proliferation, as well as the differentiation into osteoblasts. It was also demonstrated that the nHAC/PLGA scaffolds implanted into a rabbit critical-size mandible defect possessed tissue compatibility and higher bone restoration capacity compared with the defects that were filled with or without nHAC scaffolds. Furthermore, the in vivo results showed that the nHAC/PLGA scaffolds which incorporated insulin-loaded microspheres with a size of 1.61 mu m significantly accelerated bone healing compared with two other composite scaffolds. Our study indicated that the local insulin released at the optimal time could substantially and reproducibly improve bone repair.
KeywordInsulin Composite Scaffold Drug Delivery System Bone Tissue Engineering Plga Hydroxyapatite
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1021/acsami.6b13566
Indexed BySCI
Language英语
WOS KeywordMESENCHYMAL STEM-CELLS ; OSTEOGENIC DIFFERENTIATION ; RECEPTOR SUBSTRATE-1 ; STROMAL CELLS ; HYDROXYAPATITE ; DELIVERY ; EXPRESSION ; OSTEOBLAST ; NANOPARTICLES ; PROLIFERATION
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
Funding OrganizationNational Natural Science Foundation of China(81271180 ; Beijing Natural Science Foundation(7164297) ; 81150019)
WOS IDWOS:000398764100011
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22481
Collection生化工程国家重点实验室
Affiliation1.Chinese Peoples Liberat Army Gen Hosp, Inst Stomatol, Beijing 100853, Peoples R China
2.Shanxi Med Univ, Hosp Stomatol, Taiyuan 030001, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xing,Wu, Xia,Xing, Helin,et al. Porous Nanohydroxyapatite/Collagen Scaffolds Loading Insulin PLGA Particles for Restoration of Critical Size Bone Defect[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(13):11380-11391.
APA Wang, Xing.,Wu, Xia.,Xing, Helin.,Zhang, Guilan.,Shi, Quan.,...&Liu, Hongchen.(2017).Porous Nanohydroxyapatite/Collagen Scaffolds Loading Insulin PLGA Particles for Restoration of Critical Size Bone Defect.ACS APPLIED MATERIALS & INTERFACES,9(13),11380-11391.
MLA Wang, Xing,et al."Porous Nanohydroxyapatite/Collagen Scaffolds Loading Insulin PLGA Particles for Restoration of Critical Size Bone Defect".ACS APPLIED MATERIALS & INTERFACES 9.13(2017):11380-11391.
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