| Porous Nanohydroxyapatite/Collagen Scaffolds Loading Insulin PLGA Particles for Restoration of Critical Size Bone Defect | |
| Wang, Xing1,2; Wu, Xia1; Xing, Helin1; Zhang, Guilan1; Shi, Quan1; E, Lingling1; Liu, Na1; Yang, Tingyuan3; Wang, Dongsheng1; Qi, Feng3; Wang, Lianyan3; Liu, Hongchen1 | |
| 2017-04-05 | |
| Source Publication | ACS APPLIED MATERIALS & INTERFACES
 |
| ISSN | 1944-8244 |
| Volume | 9Issue:13Pages:11380-11391 |
| Abstract | Insulin is considered to be a classical central regulator of energy homeostasis. Recently, the effect of insulin on bone has gained a lot of attention, but little attention has been paid to the application in bone tissue engineering. In this study, porous nanohydroxyapatite/collagen (nHAC) scaffolds incorporating poly lactic-co-glycolic acid (PLGA) particles were successfully developed as an insulin delivery platform for bone regeneration. Bioactive insulin was successfully released from the PLGA particles within the scaffold, and the size of the particles as well as the release kinetics of the insulin could be efficiently controlled through Shirasu porous glass premix membrane emulsification technology. It was indicated that the nHAC/PLGA composite scaffolds possessed favorable mechanical and structural properties for cell adhesion and proliferation, as well as the differentiation into osteoblasts. It was also demonstrated that the nHAC/PLGA scaffolds implanted into a rabbit critical-size mandible defect possessed tissue compatibility and higher bone restoration capacity compared with the defects that were filled with or without nHAC scaffolds. Furthermore, the in vivo results showed that the nHAC/PLGA scaffolds which incorporated insulin-loaded microspheres with a size of 1.61 mu m significantly accelerated bone healing compared with two other composite scaffolds. Our study indicated that the local insulin released at the optimal time could substantially and reproducibly improve bone repair. |
| Keyword | Insulin Composite Scaffold Drug Delivery System Bone Tissue Engineering Plga Hydroxyapatite |
| Subtype | Article |
| WOS Headings | Science & Technology ; Technology |
| DOI | 10.1021/acsami.6b13566 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | MESENCHYMAL STEM-CELLS ; OSTEOGENIC DIFFERENTIATION ; RECEPTOR SUBSTRATE-1 ; STROMAL CELLS ; HYDROXYAPATITE ; DELIVERY ; EXPRESSION ; OSTEOBLAST ; NANOPARTICLES ; PROLIFERATION |
| WOS Research Area | Science & Technology - Other Topics ; Materials Science |
| WOS Subject | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| Funding Organization | National Natural Science Foundation of China(81271180 ; Beijing Natural Science Foundation(7164297) ; 81150019) |
| WOS ID | WOS:000398764100011 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/22481 |
| Collection | 生化工程国家重点实验室 |
| Affiliation | 1.Chinese Peoples Liberat Army Gen Hosp, Inst Stomatol, Beijing 100853, Peoples R China 2.Shanxi Med Univ, Hosp Stomatol, Taiyuan 030001, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Xing,Wu, Xia,Xing, Helin,et al. Porous Nanohydroxyapatite/Collagen Scaffolds Loading Insulin PLGA Particles for Restoration of Critical Size Bone Defect[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(13):11380-11391. |
| APA | Wang, Xing.,Wu, Xia.,Xing, Helin.,Zhang, Guilan.,Shi, Quan.,...&Liu, Hongchen.(2017).Porous Nanohydroxyapatite/Collagen Scaffolds Loading Insulin PLGA Particles for Restoration of Critical Size Bone Defect.ACS APPLIED MATERIALS & INTERFACES,9(13),11380-11391. |
| MLA | Wang, Xing,et al."Porous Nanohydroxyapatite/Collagen Scaffolds Loading Insulin PLGA Particles for Restoration of Critical Size Bone Defect".ACS APPLIED MATERIALS & INTERFACES 9.13(2017):11380-11391. |
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