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Application of calcium phosphate flocculation in high-density cell culture fluid with high product titer of monoclonal antibody
Chen, Guoqiang1,2; Su, Zhiguo2; Li, Feng1; Liu, Hui Fang1
2017-05-01
Source PublicationBIOPROCESS AND BIOSYSTEMS ENGINEERING
ISSN1615-7591
Volume40Issue:5Pages:703-714
Abstract

The calcium phosphate [Ca-3(PO4)(2)] precipitation was used for improving the clarification efficiency in harvest process of the monoclonal antibody (mAb) containing cell culture fluid (CCF) with high turbidity and product titer. The flocculation conditions (concentration, addition order of flocculants, pH, and operation time), and the effect of flocculants on the mAb physical chemical properties (such as distribution of charge variants and aggregates) and process-related impurities removal (such as DNA and CHOP) were evaluated in this study. The results showed that the turbidity of CCF supernatant was significantly reduced at pH 7, 120 min with addition of phosphate ions first, while a high mAb recovery yield was kept in the CCF supernatant after flocculation. Addition of calcium ions at 15-60 mM was sufficient for flocculation in this study. A relationship between turbidity/mAb recovery yield and the concentration of calcium ions was established. More than 85% DNA in the CCF were effectively removed by the addition of optimal concentration of flocculants. Flocculation process of Ca-3(PO4)(2) is an effective pretreatment method in purification processes of mAbs from the CCF with high turbidity and product titer.

KeywordCalcium Phosphate Flocculation Monoclonal Antibody Pretreatment
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1007/s00449-017-1735-9
Indexed BySCI
Language英语
WOS KeywordPurification ; Precipitation ; Recovery ; Polyelectrolytes ; Chromatography ; Therapeutics ; Technology ; Separation ; Perfusion ; Proteins
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000399681900006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22489
Collection生化工程国家重点实验室
Affiliation1.Mabworks Biotech Co Ltd, Beijing 100176, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Chen, Guoqiang,Su, Zhiguo,Li, Feng,et al. Application of calcium phosphate flocculation in high-density cell culture fluid with high product titer of monoclonal antibody[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2017,40(5):703-714.
APA Chen, Guoqiang,Su, Zhiguo,Li, Feng,&Liu, Hui Fang.(2017).Application of calcium phosphate flocculation in high-density cell culture fluid with high product titer of monoclonal antibody.BIOPROCESS AND BIOSYSTEMS ENGINEERING,40(5),703-714.
MLA Chen, Guoqiang,et al."Application of calcium phosphate flocculation in high-density cell culture fluid with high product titer of monoclonal antibody".BIOPROCESS AND BIOSYSTEMS ENGINEERING 40.5(2017):703-714.
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