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Influence of exposed magnetic nanoparticles and their application in chemiluminescence immunoassay
Zhang, Zhi1; Guan, Yueping1; Xia, Tingting2; Du, Jiao1; Li, Tao1; Sun, Zongying1; Guo, Chen2
2017-05-05
发表期刊COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
ISSN0927-7757
卷号520页码:335-342
摘要To a certain extent, uneven coating of nanoparticles may lead to exposure of their contents. In this work, a simulation system was established and exposed Fe3O4 magnetic nanoparticles (MNPs) were used to investigate the influence of the exposed case in a chemiluminescence immunoassay. Some factors such as the concentration of MNPs, coexistence of horseradish peroxidase (HRP), different particle sizes, and various treatment methods were investigated. It was confirmed that both the bodies of nanoparticles and metallic oxides on the exposed surface have associations with the detection signal. This conclusion may provide a new way to explain the deviation emerged in a chemiluminescence immunoassay. In addition, an exposed Fe3O4 MNPs based chemiluminescence immunoassay was developed to detect human chorionic gonadotropin (hCG). Carboxyl-functionalized MNPs were modified with anti-alpha-hCG antibody, and further used as solid-phase supports for sandwich-type immunoassay. The detection limit for hCG under optimal conditions was as low as 1.184 ng/mL with a linear response range of 2-100 ng/mL and a correlation coefficient of 0.989. The proposed method could be used for the determination of hCG in serum samples, which demonstrated their application potential in rapid and cost-effective detection of biological samples. (C) 2017 Elsevier B.V. All rights reserved.
关键词Magnetic Nanoparticles Chemiluminescence Immunoassay Deviation
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
DOI10.1016/j.colsurfa.2017.01.071
收录类别SCI
语种英语
关键词[WOS]IRON-OXIDE NANOPARTICLES ; PEROXIDASE-LIKE ACTIVITY ; ENZYME-IMMUNOASSAY ; SHELL ; QUANTIFICATION ; PERFORMANCE ; SEPARATION ; SERUM
WOS研究方向Chemistry
WOS类目Chemistry, Physical
项目资助者National Natural Science Foundation of China(51274035) ; National Basic Research Program of China(2013CB632602)
WOS记录号WOS:000401401100038
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22527
专题生化工程国家重点实验室
作者单位1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
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Zhang, Zhi,Guan, Yueping,Xia, Tingting,et al. Influence of exposed magnetic nanoparticles and their application in chemiluminescence immunoassay[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2017,520:335-342.
APA Zhang, Zhi.,Guan, Yueping.,Xia, Tingting.,Du, Jiao.,Li, Tao.,...&Guo, Chen.(2017).Influence of exposed magnetic nanoparticles and their application in chemiluminescence immunoassay.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,520,335-342.
MLA Zhang, Zhi,et al."Influence of exposed magnetic nanoparticles and their application in chemiluminescence immunoassay".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 520(2017):335-342.
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