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Cations Optimization for Protein Enrichment in Rice Straw by Mixed Cultures of Neurospora crassa 14-8 and Candida utilis Using Response Surface Methodology
Jia, Jinru1; Chen, Huayou1,2; Wu, Bangguo1; Ni, Zhong1
2017-06-01
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN0273-2289
Volume182Issue:2Pages:804-817
Abstract

This study investigated the effect of cation optimization by mixed cultures of Neurospora crassa and Candida utilis on the true protein (TP) content. Firstly, to enhance the nutritional contents of rice straw (RS), two fermentation parameters (effect of inoculation ratio and inoculation time) were optimized. It was found that when C. utilis was inoculated 60 h later than N. crassa with the inoculation ratio of 1:5 (N. crassa to C. utilis), the maximum TP yield was obtained. In order to further optimize TP content, Plackett-Burman design (PBD) and Box-Behnken design (BBD) of response surface methodology (RSM) were adopted. The results of PBD indicated that Mn2+, Zn2+, and Cu2+ were the significant variables. The optimum values for the three cations determined by the BBD were as follows: Mn2+ 0.06 g/L, Zn2+ 0.15 g/L, and Cu2+ 0.2 g/L. After the optimization of RSM, a model was proposed to predict the optimum value 10.36% confirmed by the experimental result 9.84%. The TP content increased from 3.98 to 9.84%, with 147.24% of its increase rate. This study proposed an ecofriendly and economical way to convert RS into protein-enriched livestock feed.

KeywordCandida Utilis Neurospora Crassa Response Surface Methodology Solid-state Fermentation True Protein
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s12010-016-2363-6
Indexed BySCI
Language英语
WOS KeywordSolid-state Fermentation ; Trichoderma-reesei Rut-c30 ; Single-cell Protein ; White-rot Fungi ; Phanerochaete-chrysosporium ; Lignin Degradation ; Wheat-straw ; Cellulase Production ; Pleurotus-ostreatus ; Enzyme-production
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
Funding OrganizationOpen Funding Project of National Key Laboratory of Biochemical Engineering ; National Key Basic Research Program of China (973 Program)(2011CBA00800) ; Key Agriculture Support Project of Jiangsu Province, China(BE2013400)
WOS IDWOS:000402141800027
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22651
Collection生化工程国家重点实验室
Affiliation1.Jiangsu Univ, Inst Life Sci, Zhenjiang 212000, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Jia, Jinru,Chen, Huayou,Wu, Bangguo,et al. Cations Optimization for Protein Enrichment in Rice Straw by Mixed Cultures of Neurospora crassa 14-8 and Candida utilis Using Response Surface Methodology[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2017,182(2):804-817.
APA Jia, Jinru,Chen, Huayou,Wu, Bangguo,&Ni, Zhong.(2017).Cations Optimization for Protein Enrichment in Rice Straw by Mixed Cultures of Neurospora crassa 14-8 and Candida utilis Using Response Surface Methodology.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,182(2),804-817.
MLA Jia, Jinru,et al."Cations Optimization for Protein Enrichment in Rice Straw by Mixed Cultures of Neurospora crassa 14-8 and Candida utilis Using Response Surface Methodology".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 182.2(2017):804-817.
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