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Cations Optimization for Protein Enrichment in Rice Straw by Mixed Cultures of Neurospora crassa 14-8 and Candida utilis Using Response Surface Methodology
Jia, Jinru1; Chen, Huayou1,2; Wu, Bangguo1; Ni, Zhong1
2017-06-01
发表期刊APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN0273-2289
卷号182期号:2页码:804-817
摘要

This study investigated the effect of cation optimization by mixed cultures of Neurospora crassa and Candida utilis on the true protein (TP) content. Firstly, to enhance the nutritional contents of rice straw (RS), two fermentation parameters (effect of inoculation ratio and inoculation time) were optimized. It was found that when C. utilis was inoculated 60 h later than N. crassa with the inoculation ratio of 1:5 (N. crassa to C. utilis), the maximum TP yield was obtained. In order to further optimize TP content, Plackett-Burman design (PBD) and Box-Behnken design (BBD) of response surface methodology (RSM) were adopted. The results of PBD indicated that Mn2+, Zn2+, and Cu2+ were the significant variables. The optimum values for the three cations determined by the BBD were as follows: Mn2+ 0.06 g/L, Zn2+ 0.15 g/L, and Cu2+ 0.2 g/L. After the optimization of RSM, a model was proposed to predict the optimum value 10.36% confirmed by the experimental result 9.84%. The TP content increased from 3.98 to 9.84%, with 147.24% of its increase rate. This study proposed an ecofriendly and economical way to convert RS into protein-enriched livestock feed.

关键词Candida Utilis Neurospora Crassa Response Surface Methodology Solid-state Fermentation True Protein
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s12010-016-2363-6
收录类别SCI
语种英语
关键词[WOS]Solid-state Fermentation ; Trichoderma-reesei Rut-c30 ; Single-cell Protein ; White-rot Fungi ; Phanerochaete-chrysosporium ; Lignin Degradation ; Wheat-straw ; Cellulase Production ; Pleurotus-ostreatus ; Enzyme-production
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS类目Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
项目资助者Open Funding Project of National Key Laboratory of Biochemical Engineering ; National Key Basic Research Program of China (973 Program)(2011CBA00800) ; Key Agriculture Support Project of Jiangsu Province, China(BE2013400)
WOS记录号WOS:000402141800027
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22651
专题生化工程国家重点实验室
作者单位1.Jiangsu Univ, Inst Life Sci, Zhenjiang 212000, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714
Jia, Jinru,Chen, Huayou,Wu, Bangguo,et al. Cations Optimization for Protein Enrichment in Rice Straw by Mixed Cultures of Neurospora crassa 14-8 and Candida utilis Using Response Surface Methodology[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2017,182(2):804-817.
APA Jia, Jinru,Chen, Huayou,Wu, Bangguo,&Ni, Zhong.(2017).Cations Optimization for Protein Enrichment in Rice Straw by Mixed Cultures of Neurospora crassa 14-8 and Candida utilis Using Response Surface Methodology.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,182(2),804-817.
MLA Jia, Jinru,et al."Cations Optimization for Protein Enrichment in Rice Straw by Mixed Cultures of Neurospora crassa 14-8 and Candida utilis Using Response Surface Methodology".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 182.2(2017):804-817.
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