Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane | |
Fan, Jinxin1,2; Luo, Jianquan1,2; Wan, Yinhua1,2 | |
2017-09-15 | |
Source Publication | JOURNAL OF MEMBRANE SCIENCE
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ISSN | 0376-7388 |
Volume | 538Pages:68-76 |
Abstract | Enzyme purification and subsequent immobilization usually require multiple steps and consume plenty of chemicals. Traditional dipping incubation operation is lengthy and the interior of porous carrier is not fully exploited due to the diffusion barrier. To solve these problems, one-step enzyme purification and immobilization was developed based on membrane chromatography concept. A membrane adsorber was prepared by polydopamine coating on a PVDF microfiltration membrane (or on a cheap and disposable cellulose filter paper) and subsequent polyethyleneimine grafting, without any solvent usage. Laccase was selectively immobilized on such membrane adsorber (enzyme activity: 39.9 +/- 4.4 U/mL; expressed activity: 19.6 +/- 3.0%) by directly capturing it from a crude fermentation broth without pH adjustment under flow through mode. The immobilized laccase had a high purity of 92.2%, while the membrane permeability decreased due to the adsorption of laccase and impurities ("membrane fouling"). Thanks to the dominant convective transport, the enzyme loading was high and much faster than that by dipping incubation (5 min vs. 80 min). Moreover, enzyme reloading on membrane adsorber was easily realized by reversible desorption-adsorption process. The constructed biocatalytic membrane exhibited a commendable reusability for bisphenol A (BPA) removal only driven by its gravity. |
Keyword | Enzymatic Membrane Reactor Membrane Chromatography Laccase Membrane Adsorber Bisphenol a |
Subtype | Article |
WOS Headings | Science & Technology ; Technology ; Physical Sciences |
DOI | 10.1016/j.memsci.2017.05.053 |
Indexed By | SCI |
Language | 英语 |
WOS Keyword | Bisphenol-a Removal ; Laccase Immobilization ; Protein Adsorption ; Performance ; Exchange ; Degradation ; Carbon ; Polydopamine ; Separation ; Supports |
WOS Research Area | Engineering ; Polymer Science |
WOS Subject | Engineering, Chemical ; Polymer Science |
Funding Organization | National Natural Science Foundation of China(21506229) |
WOS ID | WOS:000402509500008 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ipe.ac.cn/handle/122111/22656 |
Collection | 生化工程国家重点实验室 |
Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
Recommended Citation GB/T 7714 | Fan, Jinxin,Luo, Jianquan,Wan, Yinhua. Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane[J]. JOURNAL OF MEMBRANE SCIENCE,2017,538:68-76. |
APA | Fan, Jinxin,Luo, Jianquan,&Wan, Yinhua.(2017).Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane.JOURNAL OF MEMBRANE SCIENCE,538,68-76. |
MLA | Fan, Jinxin,et al."Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane".JOURNAL OF MEMBRANE SCIENCE 538(2017):68-76. |
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