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Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane
Fan, Jinxin1,2; Luo, Jianquan1,2; Wan, Yinhua1,2
2017-09-15
Source PublicationJOURNAL OF MEMBRANE SCIENCE
ISSN0376-7388
Volume538Pages:68-76
Abstract

Enzyme purification and subsequent immobilization usually require multiple steps and consume plenty of chemicals. Traditional dipping incubation operation is lengthy and the interior of porous carrier is not fully exploited due to the diffusion barrier. To solve these problems, one-step enzyme purification and immobilization was developed based on membrane chromatography concept. A membrane adsorber was prepared by polydopamine coating on a PVDF microfiltration membrane (or on a cheap and disposable cellulose filter paper) and subsequent polyethyleneimine grafting, without any solvent usage. Laccase was selectively immobilized on such membrane adsorber (enzyme activity: 39.9 +/- 4.4 U/mL; expressed activity: 19.6 +/- 3.0%) by directly capturing it from a crude fermentation broth without pH adjustment under flow through mode. The immobilized laccase had a high purity of 92.2%, while the membrane permeability decreased due to the adsorption of laccase and impurities ("membrane fouling"). Thanks to the dominant convective transport, the enzyme loading was high and much faster than that by dipping incubation (5 min vs. 80 min). Moreover, enzyme reloading on membrane adsorber was easily realized by reversible desorption-adsorption process. The constructed biocatalytic membrane exhibited a commendable reusability for bisphenol A (BPA) removal only driven by its gravity.

KeywordEnzymatic Membrane Reactor Membrane Chromatography Laccase Membrane Adsorber Bisphenol a
SubtypeArticle
WOS HeadingsScience & Technology ; Technology ; Physical Sciences
DOI10.1016/j.memsci.2017.05.053
Indexed BySCI
Language英语
WOS KeywordBisphenol-a Removal ; Laccase Immobilization ; Protein Adsorption ; Performance ; Exchange ; Degradation ; Carbon ; Polydopamine ; Separation ; Supports
WOS Research AreaEngineering ; Polymer Science
WOS SubjectEngineering, Chemical ; Polymer Science
Funding OrganizationNational Natural Science Foundation of China(21506229)
WOS IDWOS:000402509500008
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22656
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Fan, Jinxin,Luo, Jianquan,Wan, Yinhua. Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane[J]. JOURNAL OF MEMBRANE SCIENCE,2017,538:68-76.
APA Fan, Jinxin,Luo, Jianquan,&Wan, Yinhua.(2017).Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane.JOURNAL OF MEMBRANE SCIENCE,538,68-76.
MLA Fan, Jinxin,et al."Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane".JOURNAL OF MEMBRANE SCIENCE 538(2017):68-76.
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