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题名:
Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane
作者: Fan, Jinxin1,2; Luo, Jianquan1,2; Wan, Yinhua1,2
刊名: JOURNAL OF MEMBRANE SCIENCE
出版日期: 2017-09-15
卷号: 538, 页码:68-76
关键词: Enzymatic membrane reactor ; Membrane chromatography ; Laccase ; Membrane adsorber ; Bisphenol A
收录类别: SCI
WOS记录号: WOS:000402509500008
DOI: 10.1016/j.memsci.2017.05.053
文章类型: Article
英文摘要: Enzyme purification and subsequent immobilization usually require multiple steps and consume plenty of chemicals. Traditional dipping incubation operation is lengthy and the interior of porous carrier is not fully exploited due to the diffusion barrier. To solve these problems, one-step enzyme purification and immobilization was developed based on membrane chromatography concept. A membrane adsorber was prepared by polydopamine coating on a PVDF microfiltration membrane (or on a cheap and disposable cellulose filter paper) and subsequent polyethyleneimine grafting, without any solvent usage. Laccase was selectively immobilized on such membrane adsorber (enzyme activity: 39.9 +/- 4.4 U/mL; expressed activity: 19.6 +/- 3.0%) by directly capturing it from a crude fermentation broth without pH adjustment under flow through mode. The immobilized laccase had a high purity of 92.2%, while the membrane permeability decreased due to the adsorption of laccase and impurities ("membrane fouling"). Thanks to the dominant convective transport, the enzyme loading was high and much faster than that by dipping incubation (5 min vs. 80 min). Moreover, enzyme reloading on membrane adsorber was easily realized by reversible desorption-adsorption process. The constructed biocatalytic membrane exhibited a commendable reusability for bisphenol A (BPA) removal only driven by its gravity.
WOS标题词: Science & Technology ; Technology ; Physical Sciences
类目[WOS]: Engineering, Chemical ; Polymer Science
研究领域[WOS]: Engineering ; Polymer Science
关键词[WOS]: BISPHENOL-A REMOVAL ; LACCASE IMMOBILIZATION ; PROTEIN ADSORPTION ; PERFORMANCE ; EXCHANGE ; DEGRADATION ; CARBON ; POLYDOPAMINE ; SEPARATION ; SUPPORTS
项目资助者: National Natural Science Foundation of China(21506229) ; "100 Talents Program" of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017069)
语种: 英语
ISSN号: 0376-7388
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内容类型: 期刊论文
URI标识: http://ir.ipe.ac.cn/handle/122111/22656
Appears in Collections:生化工程国家重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Fan, Jinxin,Luo, Jianquan,Wan, Yinhua. Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane[J]. JOURNAL OF MEMBRANE SCIENCE,2017,538:68-76.
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