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Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane
Fan, Jinxin1,2; Luo, Jianquan1,2; Wan, Yinhua1,2
2017-09-15
发表期刊JOURNAL OF MEMBRANE SCIENCE
ISSN0376-7388
卷号538页码:68-76
摘要Enzyme purification and subsequent immobilization usually require multiple steps and consume plenty of chemicals. Traditional dipping incubation operation is lengthy and the interior of porous carrier is not fully exploited due to the diffusion barrier. To solve these problems, one-step enzyme purification and immobilization was developed based on membrane chromatography concept. A membrane adsorber was prepared by polydopamine coating on a PVDF microfiltration membrane (or on a cheap and disposable cellulose filter paper) and subsequent polyethyleneimine grafting, without any solvent usage. Laccase was selectively immobilized on such membrane adsorber (enzyme activity: 39.9 +/- 4.4 U/mL; expressed activity: 19.6 +/- 3.0%) by directly capturing it from a crude fermentation broth without pH adjustment under flow through mode. The immobilized laccase had a high purity of 92.2%, while the membrane permeability decreased due to the adsorption of laccase and impurities ("membrane fouling"). Thanks to the dominant convective transport, the enzyme loading was high and much faster than that by dipping incubation (5 min vs. 80 min). Moreover, enzyme reloading on membrane adsorber was easily realized by reversible desorption-adsorption process. The constructed biocatalytic membrane exhibited a commendable reusability for bisphenol A (BPA) removal only driven by its gravity.
关键词Enzymatic Membrane Reactor Membrane Chromatography Laccase Membrane Adsorber Bisphenol a
文章类型Article
WOS标题词Science & Technology ; Technology ; Physical Sciences
DOI10.1016/j.memsci.2017.05.053
收录类别SCI
语种英语
关键词[WOS]BISPHENOL-A REMOVAL ; LACCASE IMMOBILIZATION ; PROTEIN ADSORPTION ; PERFORMANCE ; EXCHANGE ; DEGRADATION ; CARBON ; POLYDOPAMINE ; SEPARATION ; SUPPORTS
WOS研究方向Engineering ; Polymer Science
WOS类目Engineering, Chemical ; Polymer Science
项目资助者National Natural Science Foundation of China(21506229) ; "100 Talents Program" of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017069)
WOS记录号WOS:000402509500008
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22656
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Fan, Jinxin,Luo, Jianquan,Wan, Yinhua. Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane[J]. JOURNAL OF MEMBRANE SCIENCE,2017,538:68-76.
APA Fan, Jinxin,Luo, Jianquan,&Wan, Yinhua.(2017).Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane.JOURNAL OF MEMBRANE SCIENCE,538,68-76.
MLA Fan, Jinxin,et al."Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane".JOURNAL OF MEMBRANE SCIENCE 538(2017):68-76.
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