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Chitosan oligosaccharides inhibit epithelial cell migration through blockade of N-acetylglucosaminyltransferase V and branched GlcNAc structure
Xu, Qingsong1; Wang, Wenjing2; Qu, Chen1; Gu, Jianguo3; Yin, Heng4; Jia, Zhihao1; Song, Linsheng1; Du, Yuguang5
2017-08-15
Source PublicationCARBOHYDRATE POLYMERS
ISSN0144-8617
Volume170Pages:241-246
Abstract

Chitosan oligosaccharides (COS) have been shown to regulate various cellular and biological functions. The aim of this study was to investigate the antimetastatic potency of COS and the underlying mechanism. Here, we established a stably N-acetylglucosaminyltransferase V (GnT-V)-overexpressed MCF10A cell line. As expected, GnT-V overexpression greatly promoted cell migration in the transfectants by using wound healing assay. However, the induction in the cell migration was significantly suppressed by an addition of COS. Curiously, COS inhibited the protein expression of GnT-V in a dose dependent manner. Consistent with that, the reactivities with datura stramonium (DSA) and leuko-agglutinating phytohemagglutinin (L4-PHA) lectins, which specifically recognize branched N-acetylglucosamine (Gic-NAc) structure, were also suppressed by COS. Taken together, these results demonstrated COS inhibited breast epithelial cell migration through down-regulation of GnT-V and its products, branched N-glycans, indicating that COS may serve as a potential novel therapeutic candidate for the treatment of metastatic breast cancer. (C) 2017 Elsevier Ltd. All rights reserved.

KeywordChitosan Oligosaccharides N-acetylglucosaminyltransferase v Branched N-glycan Cell Migration
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1016/j.carbpol.2017.04.075
Indexed BySCI
Language英语
WOS KeywordMolecular-weight ; Cancer-cells ; Tumor-growth ; Kappa-b ; Gnt-v ; Chitooligosaccharides ; Metastasis ; Adhesion ; Suppression ; Secretase
WOS Research AreaChemistry ; Polymer Science
WOS SubjectChemistry, Applied ; Chemistry, Organic ; Polymer Science
Funding OrganizationNational Natural Science Foundation of China(31372347 ; 31670807)
WOS IDWOS:000402942900028
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22660
Collection研究所(批量导入)
Affiliation1.Dalian Ocean Univ, Coll Fisheries & Life Sci, 52 Heishijiao St, Dalian 116023, Peoples R China
2.Dalian Elite Analyt Instruments Co Ltd, Dalian 116023, Peoples R China
3.Tohoku Med & Pharmaceut Univ, Sendai, Miyagi 9818558, Japan
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
5.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Xu, Qingsong,Wang, Wenjing,Qu, Chen,et al. Chitosan oligosaccharides inhibit epithelial cell migration through blockade of N-acetylglucosaminyltransferase V and branched GlcNAc structure[J]. CARBOHYDRATE POLYMERS,2017,170:241-246.
APA Xu, Qingsong.,Wang, Wenjing.,Qu, Chen.,Gu, Jianguo.,Yin, Heng.,...&Du, Yuguang.(2017).Chitosan oligosaccharides inhibit epithelial cell migration through blockade of N-acetylglucosaminyltransferase V and branched GlcNAc structure.CARBOHYDRATE POLYMERS,170,241-246.
MLA Xu, Qingsong,et al."Chitosan oligosaccharides inhibit epithelial cell migration through blockade of N-acetylglucosaminyltransferase V and branched GlcNAc structure".CARBOHYDRATE POLYMERS 170(2017):241-246.
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