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| Chitosan oligosaccharides inhibit epithelial cell migration through blockade of N-acetylglucosaminyltransferase V and branched GlcNAc structure | |
| Xu, Qingsong1; Wang, Wenjing2; Qu, Chen1; Gu, Jianguo3; Yin, Heng4; Jia, Zhihao1; Song, Linsheng1; Du, Yuguang5 | |
| 2017-08-15 | |
| Source Publication | CARBOHYDRATE POLYMERS
 |
| ISSN | 0144-8617 |
| Volume | 170Pages:241-246 |
| Abstract | Chitosan oligosaccharides (COS) have been shown to regulate various cellular and biological functions. The aim of this study was to investigate the antimetastatic potency of COS and the underlying mechanism. Here, we established a stably N-acetylglucosaminyltransferase V (GnT-V)-overexpressed MCF10A cell line. As expected, GnT-V overexpression greatly promoted cell migration in the transfectants by using wound healing assay. However, the induction in the cell migration was significantly suppressed by an addition of COS. Curiously, COS inhibited the protein expression of GnT-V in a dose dependent manner. Consistent with that, the reactivities with datura stramonium (DSA) and leuko-agglutinating phytohemagglutinin (L4-PHA) lectins, which specifically recognize branched N-acetylglucosamine (Gic-NAc) structure, were also suppressed by COS. Taken together, these results demonstrated COS inhibited breast epithelial cell migration through down-regulation of GnT-V and its products, branched N-glycans, indicating that COS may serve as a potential novel therapeutic candidate for the treatment of metastatic breast cancer. (C) 2017 Elsevier Ltd. All rights reserved. |
| Keyword | Chitosan Oligosaccharides N-acetylglucosaminyltransferase v Branched N-glycan Cell Migration |
| Subtype | Article |
| WOS Headings | Science & Technology ; Physical Sciences |
| DOI | 10.1016/j.carbpol.2017.04.075 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | Molecular-weight ; Cancer-cells ; Tumor-growth ; Kappa-b ; Gnt-v ; Chitooligosaccharides ; Metastasis ; Adhesion ; Suppression ; Secretase |
| WOS Research Area | Chemistry ; Polymer Science |
| WOS Subject | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| Funding Organization | National Natural Science Foundation of China(31372347 ; 31670807) |
| WOS ID | WOS:000402942900028 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/22660 |
| Collection | 研究所（批量导入） |
| Affiliation | 1.Dalian Ocean Univ, Coll Fisheries & Life Sci, 52 Heishijiao St, Dalian 116023, Peoples R China 2.Dalian Elite Analyt Instruments Co Ltd, Dalian 116023, Peoples R China 3.Tohoku Med & Pharmaceut Univ, Sendai, Miyagi 9818558, Japan 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 5.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Xu, Qingsong,Wang, Wenjing,Qu, Chen,et al. Chitosan oligosaccharides inhibit epithelial cell migration through blockade of N-acetylglucosaminyltransferase V and branched GlcNAc structure[J]. CARBOHYDRATE POLYMERS,2017,170:241-246. |
| APA | Xu, Qingsong.,Wang, Wenjing.,Qu, Chen.,Gu, Jianguo.,Yin, Heng.,...&Du, Yuguang.(2017).Chitosan oligosaccharides inhibit epithelial cell migration through blockade of N-acetylglucosaminyltransferase V and branched GlcNAc structure.CARBOHYDRATE POLYMERS,170,241-246. |
| MLA | Xu, Qingsong,et al."Chitosan oligosaccharides inhibit epithelial cell migration through blockade of N-acetylglucosaminyltransferase V and branched GlcNAc structure".CARBOHYDRATE POLYMERS 170(2017):241-246. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Chitosan oligosaccha（2093KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | Application Full Text | |
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