CAS OpenIR  > 生化工程国家重点实验室
Conjugation Reaction with 8-Arm PEG Markedly Improves the Immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 Fusion Protein
Sun, Xiaowei1,2; Yu, Weili2; Pang, Quanhai1; Hu, Tao2
2017-06-01
Source PublicationBIOCONJUGATE CHEMISTRY
ISSN1043-1802
Volume28Issue:6Pages:1658-1668
Abstract

Mycobacterium tuberculosis (Mtb) is a serious fatal pathogen responsible for tuberculosis (TB). Effective vaccination is highly desired for immunoprotection against Mtb infection. CFP10 and TB10.4 are two important immunodominant Mtb-secreted protein antigens, which suffer from poor immunogenicity. Thus, an antigen delivery system and adjuvants are needed to improve the immunogenicity of the two proteins. A CFP10-TB10.4 fusion protein (CT) was used as the antigen in the present study. Conjugation of 4-6 CT molecules in one entity with 8-arm polyethylene glycol (PEG) acted as an antigen delivery system. Aluminum-loxoribine mixture (A-L) and poly(I:C) functioned as the adjuvants. As compared with CT, the polymerized CT (CT-PEG) elicited significantly higher CT-specific IgG titers, higher Th1- and Th2-type cytokines and higher percentages of CD4(+) and CD4+ IFN-gamma(+) IL-4(+) cells in BALB/c mice. The presence of A-L and poly(I:C) could both increase the immune response to CT-PEG. Conjugation reaction with 8-arm PEG showed a predominant driving force to improve the immunogenicity of CT. Pharmacokinetic study in SD rats revealed that conjugation reaction with 8-arm PEG prolonged the systemic circulation of CT and exposure to the immune system. CT PEG with A-L showed no apparent toxicity to organs, whereas CT-PEG with poly(I:C) displayed some toxicity to organs. Thus, an effective and safe vaccine against Mtb infection could be rationally designed by conjugation reaction of Mtb-secreted protein antigen with 8-arm PEG and subsequent addition of A-L.

SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1021/acs.bioconjchem.7b00131
Indexed BySCI
Language英语
WOS KeywordMediated Immunity ; Delivery-system ; Cell-activation ; Vaccine ; Adjuvant ; Antigen ; Nanoparticles ; Infection ; Prospects ; Efficacy
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic
Funding OrganizationNational Natural Science Foundation of China(81402861 ; Beijing Natural Science Foundation(7142104) ; Science and Technology Service Network Initiative Project of Chinese Academy of Sciences(KFJ-EW-STS-027 ; 20906095) ; KFJ-EW-STS098)
WOS IDWOS:000404090500008
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22700
Collection生化工程国家重点实验室
Affiliation1.Shanxi Agr Univ, Coll Anim Sci, Taigu 030801, Shanxi Province, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Sun, Xiaowei,Yu, Weili,Pang, Quanhai,et al. Conjugation Reaction with 8-Arm PEG Markedly Improves the Immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 Fusion Protein[J]. BIOCONJUGATE CHEMISTRY,2017,28(6):1658-1668.
APA Sun, Xiaowei,Yu, Weili,Pang, Quanhai,&Hu, Tao.(2017).Conjugation Reaction with 8-Arm PEG Markedly Improves the Immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 Fusion Protein.BIOCONJUGATE CHEMISTRY,28(6),1658-1668.
MLA Sun, Xiaowei,et al."Conjugation Reaction with 8-Arm PEG Markedly Improves the Immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 Fusion Protein".BIOCONJUGATE CHEMISTRY 28.6(2017):1658-1668.
Files in This Item:
File Name/Size DocType Version Access License
Conjugation Reaction(7716KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Sun, Xiaowei]'s Articles
[Yu, Weili]'s Articles
[Pang, Quanhai]'s Articles
Baidu academic
Similar articles in Baidu academic
[Sun, Xiaowei]'s Articles
[Yu, Weili]'s Articles
[Pang, Quanhai]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Sun, Xiaowei]'s Articles
[Yu, Weili]'s Articles
[Pang, Quanhai]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.