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Denaturation of inactivated FMDV in ion exchange chromatography: Evidence by differential scanning calorimetry analysis
Liang, Shanqin1,2; Yang, Yanli1; Sun, Lijing1; Zhao, Qizu3; Ma, Guanghui1; Zhang, Songping1; Su, Zhiguo1,4
2017-08-15
发表期刊BIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
卷号124页码:99-107
摘要Removing impurities by purification is important for livestock vaccines to avoid side effects and ensure sufficient immunogenic protection on animals. In this study, three anion-exchange media with similar particle size and ligand density except pore size were applied and compared for purification of inactivated foot-and-mouth disease virus (FMDV) antigen. The dynamic binding capacity for DEAE-POROS (214 nm) and DEAE-650 M (106 nm) were 11.53 and 10.03 mg/mL, while that for DEAE-FF (32 nm) was less than 1/10 df the previous two. The recovery of inactivated FMDV after chromatographic process of these three media was 68.42%, 66.32% and 54.46%, respectively, showing a decrement as the decrease of pore size of media. Possible denaturation of the FMDV on the surfaces of anion exchange media was analyzed by differential scanning calorimetry (DSC). FMDV is known to be prone to dissociate into smaller subunits 12S. In solution, this process is reflected by Tml of DSC, which is at about 48.52 degrees C. When inactivated FMDV were absorbed on DEAE-FF, the Tml became 41.73 degrees C, indicating the increased possibility of dissociation. The TO for DEAE-650 M and DEAE-POROS was 44.04 degrees C and 45.37 degrees C, showing less dissociation and imprdved stability than on DEAE-FF. After DEAE-POROS chromatography, 94% FMDV recovery with 7.7 fold purification was achieved, further polishing by size exclusion chromatography led totally 173-fold increase in purity with average overall recovery of 79%. (C) 2017 Elsevier B.V. All rights reserved.
关键词Foot And Mouth Disease Virus Ion Exchange Chromatography Differential Scanning Calorimetry Stability Dissociation
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.bej.2017.05.004
收录类别SCI
语种英语
关键词[WOS]MOUTH-DISEASE VIRUS ; SIZE-EXCLUSION HPLC ; SURFACE-ANTIGEN ; PARTICLES ; ADSORPTION ; PROTEIN ; MEDIA ; PURIFICATION ; VACCINES ; QUANTIFICATION
WOS研究方向Biotechnology & Applied Microbiology ; Engineering
WOS类目Biotechnology & Applied Microbiology ; Engineering, Chemical
项目资助者Natural Sciences Foundation of China(21336010 ; Special Fund for Agro-scientific Research in the Public Interest(201303046) ; National Key R D Program(2016YFD0500800) ; STS Grant from Chinese Academy of Sciences(KFJ-SW-STS-162) ; 21406239)
WOS记录号WOS:000404199100012
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22707
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.China Inst Vet Drug Control, Beijing 100081, Peoples R China
4.Jiangsu Natl Synerget Innovat Ctr Adv Mat, Nanjing 211800, Jiangsu, Peoples R China
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GB/T 7714
Liang, Shanqin,Yang, Yanli,Sun, Lijing,et al. Denaturation of inactivated FMDV in ion exchange chromatography: Evidence by differential scanning calorimetry analysis[J]. BIOCHEMICAL ENGINEERING JOURNAL,2017,124:99-107.
APA Liang, Shanqin.,Yang, Yanli.,Sun, Lijing.,Zhao, Qizu.,Ma, Guanghui.,...&Su, Zhiguo.(2017).Denaturation of inactivated FMDV in ion exchange chromatography: Evidence by differential scanning calorimetry analysis.BIOCHEMICAL ENGINEERING JOURNAL,124,99-107.
MLA Liang, Shanqin,et al."Denaturation of inactivated FMDV in ion exchange chromatography: Evidence by differential scanning calorimetry analysis".BIOCHEMICAL ENGINEERING JOURNAL 124(2017):99-107.
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