CAS OpenIR  > 生化工程国家重点实验室
PEGylation with the thiosuccinimido butylamine linker significantly increases the stability of haloalkane dehalogenase DhaA
Zhao, Yuan-Zhong1; Yu, Wei-Li2; Zheng, He1; Guo, Xuan1; Guo, Nan1; Hu, Tao2; Zhong, Jin-Yi1
2017-07-20
发表期刊JOURNAL OF BIOTECHNOLOGY
ISSN0168-1656
卷号254页码:25-33
摘要

Haloalkane dehalogenase (HLD) can catalyze the hydrolytic dehalogenation of halogenated compounds. However, HLD suffers from the poor stability to resist the environmental stress. PEGylation is an effective approach to enhance the stability of enzymes. The linker is an important stabilization factor of PEGylation. Thus, the linkers of the PEGylated HLD were optimized to improve the stability of HLD in the present study. The PEGylated haloalkane dehalogenase DhaAs with methylamine (Ml), carbamate (Cm) and thiosuccinimido butylamine (Tb) linkers were prepared, respectively. The effects of the Ml, Cm and Tb linkers on the stability of the PEGylated DhaAs were investigated under different environmental stresses. Among the three linkers, the Tb linker showed the highest efficacy to improve the stability of the PEGylated DhaA. The Tb linker significantly increased the thermal stability of the PEGylated DhaA by slowing its structural unfolding, and the pH stability of the PEGylated DhaA by slowing the protonation process. In addition, the PEGylated DhaA with the Tb linker showed the maximum resistance to high ionic strength (1 M NaC1) and organic solvent (40% DMSO). PEGylation with the Tb linker is of general interest to effectively improve the stability of proteins, particularly the protein with poor stability.

关键词Protein Stability Pegylation Halogenated Compound Dhaa Linker
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jbiotec.2017.05.022
收录类别SCI
语种英语
关键词[WOS]Polymer Conjugation ; Covalent Attachment ; Polyethylene-glycol ; Proteins ; Site ; Hexachlorocyclohexane ; Crystallization ; Staphylokinase ; Enzymes ; Drug
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
项目资助者National Natural Science Foundation of China(81402861 ; Beijing Natural Science Foundation of China(7142104) ; 20906095)
WOS记录号WOS:000405154500004
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22793
专题生化工程国家重点实验室
作者单位1.Res Inst Chem Def, Beijing 102205, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Yuan-Zhong,Yu, Wei-Li,Zheng, He,et al. PEGylation with the thiosuccinimido butylamine linker significantly increases the stability of haloalkane dehalogenase DhaA[J]. JOURNAL OF BIOTECHNOLOGY,2017,254:25-33.
APA Zhao, Yuan-Zhong.,Yu, Wei-Li.,Zheng, He.,Guo, Xuan.,Guo, Nan.,...&Zhong, Jin-Yi.(2017).PEGylation with the thiosuccinimido butylamine linker significantly increases the stability of haloalkane dehalogenase DhaA.JOURNAL OF BIOTECHNOLOGY,254,25-33.
MLA Zhao, Yuan-Zhong,et al."PEGylation with the thiosuccinimido butylamine linker significantly increases the stability of haloalkane dehalogenase DhaA".JOURNAL OF BIOTECHNOLOGY 254(2017):25-33.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
PEGylation with the (1583KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhao, Yuan-Zhong]的文章
[Yu, Wei-Li]的文章
[Zheng, He]的文章
百度学术
百度学术中相似的文章
[Zhao, Yuan-Zhong]的文章
[Yu, Wei-Li]的文章
[Zheng, He]的文章
必应学术
必应学术中相似的文章
[Zhao, Yuan-Zhong]的文章
[Yu, Wei-Li]的文章
[Zheng, He]的文章
相关权益政策
暂无数据
收藏/分享
文件名: PEGylation with the thiosuccinimido butylamine linker significantly increases the stability of haloalkane dehalogenase DhaA.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。