CAS OpenIR  > 生化工程国家重点实验室
PEGylation with the thiosuccinimido butylamine linker significantly increases the stability of haloalkane dehalogenase DhaA
Zhao, Yuan-Zhong1; Yu, Wei-Li2; Zheng, He1; Guo, Xuan1; Guo, Nan1; Hu, Tao2; Zhong, Jin-Yi1
2017-07-20
Source PublicationJOURNAL OF BIOTECHNOLOGY
ISSN0168-1656
Volume254Pages:25-33
Abstract

Haloalkane dehalogenase (HLD) can catalyze the hydrolytic dehalogenation of halogenated compounds. However, HLD suffers from the poor stability to resist the environmental stress. PEGylation is an effective approach to enhance the stability of enzymes. The linker is an important stabilization factor of PEGylation. Thus, the linkers of the PEGylated HLD were optimized to improve the stability of HLD in the present study. The PEGylated haloalkane dehalogenase DhaAs with methylamine (Ml), carbamate (Cm) and thiosuccinimido butylamine (Tb) linkers were prepared, respectively. The effects of the Ml, Cm and Tb linkers on the stability of the PEGylated DhaAs were investigated under different environmental stresses. Among the three linkers, the Tb linker showed the highest efficacy to improve the stability of the PEGylated DhaA. The Tb linker significantly increased the thermal stability of the PEGylated DhaA by slowing its structural unfolding, and the pH stability of the PEGylated DhaA by slowing the protonation process. In addition, the PEGylated DhaA with the Tb linker showed the maximum resistance to high ionic strength (1 M NaC1) and organic solvent (40% DMSO). PEGylation with the Tb linker is of general interest to effectively improve the stability of proteins, particularly the protein with poor stability.

KeywordProtein Stability Pegylation Halogenated Compound Dhaa Linker
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jbiotec.2017.05.022
Indexed BySCI
Language英语
WOS KeywordPolymer Conjugation ; Covalent Attachment ; Polyethylene-glycol ; Proteins ; Site ; Hexachlorocyclohexane ; Crystallization ; Staphylokinase ; Enzymes ; Drug
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
Funding OrganizationNational Natural Science Foundation of China(81402861 ; Beijing Natural Science Foundation of China(7142104) ; 20906095)
WOS IDWOS:000405154500004
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22793
Collection生化工程国家重点实验室
Affiliation1.Res Inst Chem Def, Beijing 102205, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Yuan-Zhong,Yu, Wei-Li,Zheng, He,et al. PEGylation with the thiosuccinimido butylamine linker significantly increases the stability of haloalkane dehalogenase DhaA[J]. JOURNAL OF BIOTECHNOLOGY,2017,254:25-33.
APA Zhao, Yuan-Zhong.,Yu, Wei-Li.,Zheng, He.,Guo, Xuan.,Guo, Nan.,...&Zhong, Jin-Yi.(2017).PEGylation with the thiosuccinimido butylamine linker significantly increases the stability of haloalkane dehalogenase DhaA.JOURNAL OF BIOTECHNOLOGY,254,25-33.
MLA Zhao, Yuan-Zhong,et al."PEGylation with the thiosuccinimido butylamine linker significantly increases the stability of haloalkane dehalogenase DhaA".JOURNAL OF BIOTECHNOLOGY 254(2017):25-33.
Files in This Item:
File Name/Size DocType Version Access License
PEGylation with the (1583KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhao, Yuan-Zhong]'s Articles
[Yu, Wei-Li]'s Articles
[Zheng, He]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhao, Yuan-Zhong]'s Articles
[Yu, Wei-Li]'s Articles
[Zheng, He]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhao, Yuan-Zhong]'s Articles
[Yu, Wei-Li]'s Articles
[Zheng, He]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.