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High hydrostatic pressure encapsulation of doxorubicin in ferritin nanocages with enhanced efficiency
Wang, Qi1,2; Zhang, Chun1; Liu, Liping1,4; Lia, Zenglan1,2; Guo, Fangxia1,2; Li, Xiunan1; Luo, Jian1; Zhao, Dawei1; Liu, Yongdong1; Su, Zhiguo1,3

Human ferritin (HFn) nanocaging is becoming an appealing platform for anticancer drugs delivery. However, protein aggregation always occurs during the encapsulation process, resulting in low production efficiency. A new approach using high hydrostatic pressure (HHP) was explored in this study to overcome the problem of loading doxorubicin (DOX) in HFn. At the pressure of 500 MPa and pH 5.5, DOX molecules were found to be encapsulated into HFn. Meanwhile, combining it with an additive of 20 mM arginine completely inhibited precipitation and aggregation, resulting in highly monodispersed nanoparticles with almost 100% protein recovery. Furthermore, stepwise decompression and incubation of the complex in atmospheric pressure at pH 7.4 for another period could further increase the DOX encapsulation ratio. The HFn-DOX nanoparticles (NPs) showed similar morphology and structural features to the hollow cage and no notable drug leakage occurred for HFn-DOX NPs when stored at 4 degrees C and pH 7.4 for two weeks. HFn-DOX NPs prepared through HHP also showed significant cytotoxicity in vitro and higher antitumor bioactivity in vivo than naked DOX. Moreover, This HHP encapsulation strategy could economize on DOX that was greatly wasted during the conventional preparation process simply through a desalting column. These results indicated that HHP could offer a feasible approach with high efficiency for the production of HFn-DOX NPs.

KeywordHigh Hydrostatic Pressure Anticancer Drug Human Ferritin Doxorubicin Encapsulation Nanoparticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
WOS KeywordH-ferritin ; Protein Nanocages ; Drug-delivery ; Apoferritin ; Nanoparticles ; Dissociation ; Adriamycin ; Complexes ; Hydration ; Cavities
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
Funding OrganizationNational Natural Science Foundation of China(21576267 ; Beijing Natural Science Foundation(2162041) ; Major State Basic Research Development Program of China(2013CB733604) ; Open Funding Project of the National Key Laboratory of Biochemical Engineering(2014KF-05) ; 21506228)
WOS IDWOS:000405154500005
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Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, 1 Beierjie St, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing 210023, Jiangsu, Peoples R China
4.Sichuan Univ, West China Sch Pharm, Minist Educ, Key Lab Drug Targeting & Drug Delivery Syst, Chengdu 610041, Peoples R China
Recommended Citation
GB/T 7714
Wang, Qi,Zhang, Chun,Liu, Liping,et al. High hydrostatic pressure encapsulation of doxorubicin in ferritin nanocages with enhanced efficiency[J]. JOURNAL OF BIOTECHNOLOGY,2017,254:34-42.
APA Wang, Qi.,Zhang, Chun.,Liu, Liping.,Lia, Zenglan.,Guo, Fangxia.,...&Su, Zhiguo.(2017).High hydrostatic pressure encapsulation of doxorubicin in ferritin nanocages with enhanced efficiency.JOURNAL OF BIOTECHNOLOGY,254,34-42.
MLA Wang, Qi,et al."High hydrostatic pressure encapsulation of doxorubicin in ferritin nanocages with enhanced efficiency".JOURNAL OF BIOTECHNOLOGY 254(2017):34-42.
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