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High hydrostatic pressure encapsulation of doxorubicin in ferritin nanocages with enhanced efficiency
Wang, Qi1,2; Zhang, Chun1; Liu, Liping1,4; Lia, Zenglan1,2; Guo, Fangxia1,2; Li, Xiunan1; Luo, Jian1; Zhao, Dawei1; Liu, Yongdong1; Su, Zhiguo1,3
2017-07-20
发表期刊JOURNAL OF BIOTECHNOLOGY
ISSN0168-1656
卷号254页码:34-42
摘要Human ferritin (HFn) nanocaging is becoming an appealing platform for anticancer drugs delivery. However, protein aggregation always occurs during the encapsulation process, resulting in low production efficiency. A new approach using high hydrostatic pressure (HHP) was explored in this study to overcome the problem of loading doxorubicin (DOX) in HFn. At the pressure of 500 MPa and pH 5.5, DOX molecules were found to be encapsulated into HFn. Meanwhile, combining it with an additive of 20 mM arginine completely inhibited precipitation and aggregation, resulting in highly monodispersed nanoparticles with almost 100% protein recovery. Furthermore, stepwise decompression and incubation of the complex in atmospheric pressure at pH 7.4 for another period could further increase the DOX encapsulation ratio. The HFn-DOX nanoparticles (NPs) showed similar morphology and structural features to the hollow cage and no notable drug leakage occurred for HFn-DOX NPs when stored at 4 degrees C and pH 7.4 for two weeks. HFn-DOX NPs prepared through HHP also showed significant cytotoxicity in vitro and higher antitumor bioactivity in vivo than naked DOX. Moreover, This HHP encapsulation strategy could economize on DOX that was greatly wasted during the conventional preparation process simply through a desalting column. These results indicated that HHP could offer a feasible approach with high efficiency for the production of HFn-DOX NPs.
关键词High Hydrostatic Pressure Anticancer Drug Human Ferritin Doxorubicin Encapsulation Nanoparticle
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jbiotec.2017.05.025
收录类别SCI
语种英语
关键词[WOS]H-FERRITIN ; PROTEIN NANOCAGES ; DRUG-DELIVERY ; APOFERRITIN ; NANOPARTICLES ; DISSOCIATION ; ADRIAMYCIN ; COMPLEXES ; HYDRATION ; CAVITIES
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
项目资助者National Natural Science Foundation of China(21576267 ; Beijing Natural Science Foundation(2162041) ; Major State Basic Research Development Program of China(2013CB733604) ; Open Funding Project of the National Key Laboratory of Biochemical Engineering(2014KF-05) ; 21506228)
WOS记录号WOS:000405154500005
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22804
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, 1 Beierjie St, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing 210023, Jiangsu, Peoples R China
4.Sichuan Univ, West China Sch Pharm, Minist Educ, Key Lab Drug Targeting & Drug Delivery Syst, Chengdu 610041, Peoples R China
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Wang, Qi,Zhang, Chun,Liu, Liping,et al. High hydrostatic pressure encapsulation of doxorubicin in ferritin nanocages with enhanced efficiency[J]. JOURNAL OF BIOTECHNOLOGY,2017,254:34-42.
APA Wang, Qi.,Zhang, Chun.,Liu, Liping.,Lia, Zenglan.,Guo, Fangxia.,...&Su, Zhiguo.(2017).High hydrostatic pressure encapsulation of doxorubicin in ferritin nanocages with enhanced efficiency.JOURNAL OF BIOTECHNOLOGY,254,34-42.
MLA Wang, Qi,et al."High hydrostatic pressure encapsulation of doxorubicin in ferritin nanocages with enhanced efficiency".JOURNAL OF BIOTECHNOLOGY 254(2017):34-42.
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