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High-level productivity of alpha,omega-dodecanedioic acid with a newly isolated Candida viswanathii strain
Cao, Weifeng1; Li, Hongbao1,2; Luo, Jianquan1; Yin, Junxiang3; Wan, Yinhua1
2017-08-01
Source PublicationJOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
ISSN1367-5435
Volume44Issue:8Pages:1191-1202
Abstract

alpha,omega-Dicarboxylic acids (DC) are versatile chemical intermediates with different chain lengths, which are well-known as polymer building block. In this work, a new strain with high productivity of DC was isolated from oil-contaminated soil. Based on the morphology and phylogenetic analyses of the internal transcribed spacer sequences, it was characterized as Candida viswanathii. It was found that the contribution of carbon flux to the cell growth and DC production from n-dodecane could be regulated by the sucrose and yeast extract concentrations in the medium, and besides the broth pH, a suitable proportioning of sucrose and yeast extract was the key to achieve the optimal transition from cell growth phase to DC production phase. By optimizing culture conditions in a 7.5-L bioreactor, a higher DC productivity of 1.59 g center dot L-1 h(-1) with a corresponding concentration of 181.6 g/L was obtained. After the purification of DC from the culture, the results from gas chromatography-mass spectrometry, infrared spectroscopy and H-1-NMR showed that alpha,omega-dodecanedioic acid (DC12) was the major product of C. viswanathii ipe-1 using pure n-dodecane as substrate. For the first time, we reported that a high productivity of DC12 could be produced by C. viswanathii.

KeywordCandida Viswanathii Alpha Omega-dodecanedioic Acid N-alkane Alpha Optimal Strategy Omega-dicarboxylic Acids
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10295-017-1948-6
Indexed BySCI
Language英语
WOS KeywordChain Dicarboxylic-acids ; Yarrowia-lipolytica ; N-alkanes ; Tropicalis ; Mutant
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
Funding OrganizationNational High Technology Research and Development Program of China(2015AA021002 ; National Science Foundation of China(21406240) ; Chinese Academy of Sciences' Key Deployment Project(KSZD-EW-2-017-3) ; 2014AA021005)
WOS IDWOS:000405498900007
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22805
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Shijiazhuang 050018, Peoples R China
3.China Natl Ctr Biotechnol Dev, Beijing 100036, Peoples R China
Recommended Citation
GB/T 7714
Cao, Weifeng,Li, Hongbao,Luo, Jianquan,et al. High-level productivity of alpha,omega-dodecanedioic acid with a newly isolated Candida viswanathii strain[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2017,44(8):1191-1202.
APA Cao, Weifeng,Li, Hongbao,Luo, Jianquan,Yin, Junxiang,&Wan, Yinhua.(2017).High-level productivity of alpha,omega-dodecanedioic acid with a newly isolated Candida viswanathii strain.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,44(8),1191-1202.
MLA Cao, Weifeng,et al."High-level productivity of alpha,omega-dodecanedioic acid with a newly isolated Candida viswanathii strain".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 44.8(2017):1191-1202.
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