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High-level productivity of alpha,omega-dodecanedioic acid with a newly isolated Candida viswanathii strain
Cao, Weifeng1; Li, Hongbao1,2; Luo, Jianquan1; Yin, Junxiang3; Wan, Yinhua1
2017-08-01
发表期刊JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
ISSN1367-5435
卷号44期号:8页码:1191-1202
摘要alpha,omega-Dicarboxylic acids (DC) are versatile chemical intermediates with different chain lengths, which are well-known as polymer building block. In this work, a new strain with high productivity of DC was isolated from oil-contaminated soil. Based on the morphology and phylogenetic analyses of the internal transcribed spacer sequences, it was characterized as Candida viswanathii. It was found that the contribution of carbon flux to the cell growth and DC production from n-dodecane could be regulated by the sucrose and yeast extract concentrations in the medium, and besides the broth pH, a suitable proportioning of sucrose and yeast extract was the key to achieve the optimal transition from cell growth phase to DC production phase. By optimizing culture conditions in a 7.5-L bioreactor, a higher DC productivity of 1.59 g center dot L-1 h(-1) with a corresponding concentration of 181.6 g/L was obtained. After the purification of DC from the culture, the results from gas chromatography-mass spectrometry, infrared spectroscopy and H-1-NMR showed that alpha,omega-dodecanedioic acid (DC12) was the major product of C. viswanathii ipe-1 using pure n-dodecane as substrate. For the first time, we reported that a high productivity of DC12 could be produced by C. viswanathii.
关键词Candida Viswanathii Alpha Omega-dodecanedioic Acid N-alkane Alpha Optimal Strategy Omega-dicarboxylic Acids
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10295-017-1948-6
收录类别SCI
语种英语
关键词[WOS]CHAIN DICARBOXYLIC-ACIDS ; YARROWIA-LIPOLYTICA ; N-ALKANES ; TROPICALIS ; MUTANT
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
项目资助者National High Technology Research and Development Program of China(2015AA021002 ; National Science Foundation of China(21406240) ; Chinese Academy of Sciences' Key Deployment Project(KSZD-EW-2-017-3) ; 2014AA021005)
WOS记录号WOS:000405498900007
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22805
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Shijiazhuang 050018, Peoples R China
3.China Natl Ctr Biotechnol Dev, Beijing 100036, Peoples R China
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GB/T 7714
Cao, Weifeng,Li, Hongbao,Luo, Jianquan,et al. High-level productivity of alpha,omega-dodecanedioic acid with a newly isolated Candida viswanathii strain[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2017,44(8):1191-1202.
APA Cao, Weifeng,Li, Hongbao,Luo, Jianquan,Yin, Junxiang,&Wan, Yinhua.(2017).High-level productivity of alpha,omega-dodecanedioic acid with a newly isolated Candida viswanathii strain.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,44(8),1191-1202.
MLA Cao, Weifeng,et al."High-level productivity of alpha,omega-dodecanedioic acid with a newly isolated Candida viswanathii strain".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 44.8(2017):1191-1202.
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