CAS OpenIR  > 生化工程国家重点实验室
Stabilization study of inactivated foot and mouth disease virus vaccine by size-exclusion HPLC and differential scanning calorimetry
Yang, Yanli1; Zhao, Qizu3; Li, Zhengjun1,2; Sun, Lijing1; Ma, Guanghui1; Zhang, Songping1; Su, Zhiguo1
2017-04-25
Source PublicationVACCINE
ISSN0264-410X
Volume35Issue:18Pages:2413-2419
AbstractThe inactivated foot and mouth disease virus (FMDV), which has a sedimentation coefficient of 146S, is crucial to the efficacy of vaccine preparations, but extremely unstable in vitro. It is prone to dissociate into smaller particles referred to as 12S with a concomitant decrease in immunogenicity; therefore, it is of great importance to find the best condition for stabilizing the FMDV. In the present work, the effects of solution pH and temperature on the dissociation of 146S was investigated and potential stabilizers were screened, with aid of high-performance size-exclusion chromatography (HPSEC) for rapid and quantitative determination of 146S, together with differential scanning calorimetry (DSC) technology for thermal stability analysis. The most stable pH was found between 7.5 and 8.0. Among excipients tested, sucrose and glycerol provided the best protection, such that the half-life of 146S in solution at 45 degrees C could be prolonged from less than 30 min to more than 3 days by adding 20% sucrose. The stabilization mechanism was confirmed using DSC analysis, which showed that the transition temperature related to 146S dissociation was increased by 5.4 degrees C in the presence of 20% sucrose. The physical stabilization effects afforded by these stabilizers would allow for the retaining of effective 146S antigens during transportation and storage under relative harsh condition. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordFoot And Mouth Disease Virus Thermal Stability Dissociation Stabilization Excipient
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.vaccine.2017.03.037
Indexed BySCI
Language英语
WOS KeywordPREFERENTIAL HYDRATION ; 12S PARTICLES ; STABILITY ; 146S ; QUANTIFICATION ; CHROMATOGRAPHY ; DISSOCIATION ; GLYCEROL ; STORAGE ; ASIA-1
WOS Research AreaImmunology ; Research & Experimental Medicine
WOS SubjectImmunology ; Medicine, Research & Experimental
Funding OrganizationNatural Sciences Foundation of China(21336010 ; Special Fund for Agro-scientific Research in the Public Interest(201303046) ; National Key R D Program(2016YFD0500809) ; STS Grant from the Chinese Academy of Sciences(KFJ-SW-STS-162) ; 21406239)
WOS IDWOS:000400715600021
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22824
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.China Inst Vet Drug Control, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Yang, Yanli,Zhao, Qizu,Li, Zhengjun,et al. Stabilization study of inactivated foot and mouth disease virus vaccine by size-exclusion HPLC and differential scanning calorimetry[J]. VACCINE,2017,35(18):2413-2419.
APA Yang, Yanli.,Zhao, Qizu.,Li, Zhengjun.,Sun, Lijing.,Ma, Guanghui.,...&Su, Zhiguo.(2017).Stabilization study of inactivated foot and mouth disease virus vaccine by size-exclusion HPLC and differential scanning calorimetry.VACCINE,35(18),2413-2419.
MLA Yang, Yanli,et al."Stabilization study of inactivated foot and mouth disease virus vaccine by size-exclusion HPLC and differential scanning calorimetry".VACCINE 35.18(2017):2413-2419.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yang, Yanli]'s Articles
[Zhao, Qizu]'s Articles
[Li, Zhengjun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang, Yanli]'s Articles
[Zhao, Qizu]'s Articles
[Li, Zhengjun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang, Yanli]'s Articles
[Zhao, Qizu]'s Articles
[Li, Zhengjun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.