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Stabilization study of inactivated foot and mouth disease virus vaccine by size-exclusion HPLC and differential scanning calorimetry
Yang, Yanli1; Zhao, Qizu3; Li, Zhengjun1,2; Sun, Lijing1; Ma, Guanghui1; Zhang, Songping1; Su, Zhiguo1
2017-04-25
发表期刊VACCINE
ISSN0264-410X
卷号35期号:18页码:2413-2419
摘要The inactivated foot and mouth disease virus (FMDV), which has a sedimentation coefficient of 146S, is crucial to the efficacy of vaccine preparations, but extremely unstable in vitro. It is prone to dissociate into smaller particles referred to as 12S with a concomitant decrease in immunogenicity; therefore, it is of great importance to find the best condition for stabilizing the FMDV. In the present work, the effects of solution pH and temperature on the dissociation of 146S was investigated and potential stabilizers were screened, with aid of high-performance size-exclusion chromatography (HPSEC) for rapid and quantitative determination of 146S, together with differential scanning calorimetry (DSC) technology for thermal stability analysis. The most stable pH was found between 7.5 and 8.0. Among excipients tested, sucrose and glycerol provided the best protection, such that the half-life of 146S in solution at 45 degrees C could be prolonged from less than 30 min to more than 3 days by adding 20% sucrose. The stabilization mechanism was confirmed using DSC analysis, which showed that the transition temperature related to 146S dissociation was increased by 5.4 degrees C in the presence of 20% sucrose. The physical stabilization effects afforded by these stabilizers would allow for the retaining of effective 146S antigens during transportation and storage under relative harsh condition. (C) 2017 Elsevier Ltd. All rights reserved.
关键词Foot And Mouth Disease Virus Thermal Stability Dissociation Stabilization Excipient
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.vaccine.2017.03.037
收录类别SCI
语种英语
关键词[WOS]PREFERENTIAL HYDRATION ; 12S PARTICLES ; STABILITY ; 146S ; QUANTIFICATION ; CHROMATOGRAPHY ; DISSOCIATION ; GLYCEROL ; STORAGE ; ASIA-1
WOS研究方向Immunology ; Research & Experimental Medicine
WOS类目Immunology ; Medicine, Research & Experimental
项目资助者Natural Sciences Foundation of China(21336010 ; Special Fund for Agro-scientific Research in the Public Interest(201303046) ; National Key R D Program(2016YFD0500809) ; STS Grant from the Chinese Academy of Sciences(KFJ-SW-STS-162) ; 21406239)
WOS记录号WOS:000400715600021
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/22824
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.China Inst Vet Drug Control, Beijing 100081, Peoples R China
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Yang, Yanli,Zhao, Qizu,Li, Zhengjun,et al. Stabilization study of inactivated foot and mouth disease virus vaccine by size-exclusion HPLC and differential scanning calorimetry[J]. VACCINE,2017,35(18):2413-2419.
APA Yang, Yanli.,Zhao, Qizu.,Li, Zhengjun.,Sun, Lijing.,Ma, Guanghui.,...&Su, Zhiguo.(2017).Stabilization study of inactivated foot and mouth disease virus vaccine by size-exclusion HPLC and differential scanning calorimetry.VACCINE,35(18),2413-2419.
MLA Yang, Yanli,et al."Stabilization study of inactivated foot and mouth disease virus vaccine by size-exclusion HPLC and differential scanning calorimetry".VACCINE 35.18(2017):2413-2419.
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