| Stabilization study of inactivated foot and mouth disease virus vaccine by size-exclusion HPLC and differential scanning calorimetry | |
| Yang, Yanli1; Zhao, Qizu3; Li, Zhengjun1,2; Sun, Lijing1; Ma, Guanghui1; Zhang, Songping1; Su, Zhiguo1 | |
| 2017-04-25 | |
| Source Publication | VACCINE
 |
| ISSN | 0264-410X |
| Volume | 35Issue:18Pages:2413-2419 |
| Abstract | The inactivated foot and mouth disease virus (FMDV), which has a sedimentation coefficient of 146S, is crucial to the efficacy of vaccine preparations, but extremely unstable in vitro. It is prone to dissociate into smaller particles referred to as 12S with a concomitant decrease in immunogenicity; therefore, it is of great importance to find the best condition for stabilizing the FMDV. In the present work, the effects of solution pH and temperature on the dissociation of 146S was investigated and potential stabilizers were screened, with aid of high-performance size-exclusion chromatography (HPSEC) for rapid and quantitative determination of 146S, together with differential scanning calorimetry (DSC) technology for thermal stability analysis. The most stable pH was found between 7.5 and 8.0. Among excipients tested, sucrose and glycerol provided the best protection, such that the half-life of 146S in solution at 45 degrees C could be prolonged from less than 30 min to more than 3 days by adding 20% sucrose. The stabilization mechanism was confirmed using DSC analysis, which showed that the transition temperature related to 146S dissociation was increased by 5.4 degrees C in the presence of 20% sucrose. The physical stabilization effects afforded by these stabilizers would allow for the retaining of effective 146S antigens during transportation and storage under relative harsh condition. (C) 2017 Elsevier Ltd. All rights reserved. |
| Keyword | Foot And Mouth Disease Virus Thermal Stability Dissociation Stabilization Excipient |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1016/j.vaccine.2017.03.037 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | PREFERENTIAL HYDRATION ; 12S PARTICLES ; STABILITY ; 146S ; QUANTIFICATION ; CHROMATOGRAPHY ; DISSOCIATION ; GLYCEROL ; STORAGE ; ASIA-1 |
| WOS Research Area | Immunology ; Research & Experimental Medicine |
| WOS Subject | Immunology ; Medicine, Research & Experimental |
| Funding Organization | Natural Sciences Foundation of China(21336010 ; Special Fund for Agro-scientific Research in the Public Interest(201303046) ; National Key R D Program(2016YFD0500809) ; STS Grant from the Chinese Academy of Sciences(KFJ-SW-STS-162) ; 21406239) |
| WOS ID | WOS:000400715600021 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/22824 |
| Collection | 生化工程国家重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.China Inst Vet Drug Control, Beijing 100081, Peoples R China |
| Recommended Citation GB/T 7714 | Yang, Yanli,Zhao, Qizu,Li, Zhengjun,et al. Stabilization study of inactivated foot and mouth disease virus vaccine by size-exclusion HPLC and differential scanning calorimetry[J]. VACCINE,2017,35(18):2413-2419. |
| APA | Yang, Yanli.,Zhao, Qizu.,Li, Zhengjun.,Sun, Lijing.,Ma, Guanghui.,...&Su, Zhiguo.(2017).Stabilization study of inactivated foot and mouth disease virus vaccine by size-exclusion HPLC and differential scanning calorimetry.VACCINE,35(18),2413-2419. |
| MLA | Yang, Yanli,et al."Stabilization study of inactivated foot and mouth disease virus vaccine by size-exclusion HPLC and differential scanning calorimetry".VACCINE 35.18(2017):2413-2419. |
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