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Protective effect of polysaccharide from maca (Lepidium meyenii) on Hep-G2 cells and alcoholic liver oxidative injury in mice
Zhang, Lijun1,2; Zhao, Qingsheng1; Wang, Liwei1; Zhao, Mingxia1; Zhao, Bing1
2017-06-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume99Pages:63-70
Abstract

To study the characterization and hepatoprotective activity of polysaccharide from maca (Lepidium meyenii), the main polysaccharide from maca (MP-1) was obtained by DEAE-52 cellulose column. The average molecular weight of MP-1 was 1067.3 kDa and the polysaccharide purity was 91.63%. In order to assess the antioxidant activities of MP-1, four kinds of methods were used, including scavenging hydroxyl radical, DPPH, superoxide anion radical, and FRAP, and the results indicated high antioxidant activities. Furthermore, hepatoprotective activity of MP-1 was studied both in vitro and vivo. In vitro, the alcohol induced Hep-G2 cells model was established to evaluate the protective effect of MP-1, which demonstrated MP-1 can alleviate alcohol damage in Hep-G2 cells. In vivo, the Institute of Cancer Researcch (ICR) mice were used to evaluate hepatoprotecive effects of MP-1 on alcoholic liver disease (ALD). Supplement with MP-1 supressed the triglyceride level both in serum and in hepatic tissue. In addition, MP-1 ameliorated serous transaminases increase induced by alcohol, including aspartate transaminase, alanine aminotransferase, and gamma-glutamyl transpeptidase. Moreover, MP-1 also dramatically increased the superoxide dismutase, glutathione peroxidase, and glutathione s-transferase levels in alcoholic mice. Meantime, histopathologic results MP-1 lighten inflammation induced by alcohol. These results indicate that MP-1 possesses hepatoprotective activity against hepatic injury induced by alcohol. (C) 2017 Elsevier B.V. All rights reserved.

KeywordLepidium Meyenii Polysaccharide Alcoholic Liver Oxidative Injury Liver Protective Effect
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.ijbiomac.2017.01.125
Indexed BySCI
Language英语
WOS KeywordAntioxidant Activity ; Disease ; Extract ; Rats ; Supplementation ; Capacity ; Burden
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
Funding OrganizationNational High Technology Research and Development Program of China (863 Program)(2012AA021702-4)
WOS IDWOS:000400034100008
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/22831
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Div Biorefineiy Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Lijun,Zhao, Qingsheng,Wang, Liwei,et al. Protective effect of polysaccharide from maca (Lepidium meyenii) on Hep-G2 cells and alcoholic liver oxidative injury in mice[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2017,99:63-70.
APA Zhang, Lijun,Zhao, Qingsheng,Wang, Liwei,Zhao, Mingxia,&Zhao, Bing.(2017).Protective effect of polysaccharide from maca (Lepidium meyenii) on Hep-G2 cells and alcoholic liver oxidative injury in mice.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,99,63-70.
MLA Zhang, Lijun,et al."Protective effect of polysaccharide from maca (Lepidium meyenii) on Hep-G2 cells and alcoholic liver oxidative injury in mice".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 99(2017):63-70.
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