CAS OpenIR  > 湿法冶金清洁生产技术国家工程实验室
Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes
Yang, M.1; Mu, T.1; Zhong, W.2; Olajuyin, A. M.2; Xing, J.1
2017-08-01
Source PublicationLETTERS IN APPLIED MICROBIOLOGY
ISSN0266-8254
Volume65Issue:2Pages:165-172
Abstract

For pyruvate-producing strains, intracellular reduced nicotinamide adenine dinucleotide (NADH) accumulation is the main reason for the glycolysis inhibition. Comparing with glucose, using sodium gluconate as carbon source brought a decrease in NADH production and an increase in pyruvate production in engineered strain YP211. In order to explore the metabolic advantages of gluconate, genome-wide transcriptome analysis was employed to compare the metabolic differences between the two carbon sources. The results showed that the transcription of the genes gntU, gntK, and gntT responsible for transport and phosphorylation of gluconate, and genes edd and eda belonging to the Entner-Doudoroff (ED) pathway, was significantly enhanced. This suggested that the shortest route for the synthesis of pyruvate from gluconate was activated, and the synthesis of NADH was halved. Besides, the transcription of genes glpABCDTKF related to the glycerol metabolism was significantly enhanced, which might be because glycerol metabolism pathways were activated in the absence of glucose. These results provided valuable information for the further design of metabolic pathways in the construction of pyruvate-producing strains.

KeywordEntner-doudoroff Pathway Genome-wide Transcriptome Gluconate Metabolism Pyruvate Redox State
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1111/lam.12758
Indexed BySCI
Language英语
WOS KeywordCarbon Source ; Glycolytic Flux ; Acid ; Dehydrogenase ; Enhancement ; Glucose ; Mutant ; Growth
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
Funding OrganizationNational High Technology Research and Development Program of China(2014AA021900)
WOS IDWOS:000406935400009
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/23147
Collection湿法冶金清洁生产技术国家工程实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing, Peoples R China
2.Grad Sch Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Yang, M.,Mu, T.,Zhong, W.,et al. Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes[J]. LETTERS IN APPLIED MICROBIOLOGY,2017,65(2):165-172.
APA Yang, M.,Mu, T.,Zhong, W.,Olajuyin, A. M.,&Xing, J..(2017).Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes.LETTERS IN APPLIED MICROBIOLOGY,65(2),165-172.
MLA Yang, M.,et al."Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes".LETTERS IN APPLIED MICROBIOLOGY 65.2(2017):165-172.
Files in This Item:
File Name/Size DocType Version Access License
Analysis of gluconat(414KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yang, M.]'s Articles
[Mu, T.]'s Articles
[Zhong, W.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang, M.]'s Articles
[Mu, T.]'s Articles
[Zhong, W.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang, M.]'s Articles
[Mu, T.]'s Articles
[Zhong, W.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.