Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes
Yang, M.1; Mu, T.1; Zhong, W.2; Olajuyin, A. M.2; Xing, J.1
2017-08-01
发表期刊LETTERS IN APPLIED MICROBIOLOGY
ISSN0266-8254
卷号65期号:2页码:165-172
摘要

For pyruvate-producing strains, intracellular reduced nicotinamide adenine dinucleotide (NADH) accumulation is the main reason for the glycolysis inhibition. Comparing with glucose, using sodium gluconate as carbon source brought a decrease in NADH production and an increase in pyruvate production in engineered strain YP211. In order to explore the metabolic advantages of gluconate, genome-wide transcriptome analysis was employed to compare the metabolic differences between the two carbon sources. The results showed that the transcription of the genes gntU, gntK, and gntT responsible for transport and phosphorylation of gluconate, and genes edd and eda belonging to the Entner-Doudoroff (ED) pathway, was significantly enhanced. This suggested that the shortest route for the synthesis of pyruvate from gluconate was activated, and the synthesis of NADH was halved. Besides, the transcription of genes glpABCDTKF related to the glycerol metabolism was significantly enhanced, which might be because glycerol metabolism pathways were activated in the absence of glucose. These results provided valuable information for the further design of metabolic pathways in the construction of pyruvate-producing strains.

关键词Entner-doudoroff Pathway Genome-wide Transcriptome Gluconate Metabolism Pyruvate Redox State
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1111/lam.12758
收录类别SCI
语种英语
关键词[WOS]Carbon Source ; Glycolytic Flux ; Acid ; Dehydrogenase ; Enhancement ; Glucose ; Mutant ; Growth
WOS研究方向Biotechnology & Applied Microbiology ; Microbiology
WOS类目Biotechnology & Applied Microbiology ; Microbiology
项目资助者National High Technology Research and Development Program of China(2014AA021900)
WOS记录号WOS:000406935400009
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/23147
专题湿法冶金清洁生产技术国家工程实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing, Peoples R China
2.Grad Sch Chinese Acad Sci, Beijing, Peoples R China
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GB/T 7714
Yang, M.,Mu, T.,Zhong, W.,et al. Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes[J]. LETTERS IN APPLIED MICROBIOLOGY,2017,65(2):165-172.
APA Yang, M.,Mu, T.,Zhong, W.,Olajuyin, A. M.,&Xing, J..(2017).Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes.LETTERS IN APPLIED MICROBIOLOGY,65(2),165-172.
MLA Yang, M.,et al."Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes".LETTERS IN APPLIED MICROBIOLOGY 65.2(2017):165-172.
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