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Intracellular accumulation and immunological responses of lipid modified magnetic iron nanoparticles in mouse antigen processing cells
Qiao, Chenmeng1,2; Yang, Jun2; Chen, Lei3; Weng, Jie1; Zhang, Xin2
2017-08-01
发表期刊BIOMATERIALS SCIENCE
ISSN2047-4830
卷号5期号:8页码:1603-1611
摘要Understanding the effects of magnetic iron nanoparticles (MINPs) on the immune response is vitally important for biomedical applications such as cancer therapy, disease diagnosis and novel cancer imaging. In this study, lipid modified MINPs were designed and prepared by introducing the neutral lipid DSPE-PEG or the zwitterionic lipid DSPE-PCB into hydrophobic MINPs through hydrophobic interaction (L-MINPs and ZL-MINPs, respectively). The effect of L-MINPs and ZL-MINPs on the intracellular accumulation and immune responses of three kinds of antigen processing cells was examined. The results indicated that the high cellular uptake efficiency of surface coated MINPs was strongly related to the nature of the coating lipid, with the zwitterionic lipid being more effective than PEGylated ones. Besides, the results from flow cytometry (FCM), confocal laser scanning microscopy (CLSM) and Prussian blue staining demonstrated a time-and concentration-dependent MINP internalization. The uptake of zwitterionic lipid modified MINPs (ZL-MINPs) induced very low cytotoxicity and a strong mixed Th1/Th2 type immune response. L-MINPs could induce a strong increase in pro-inflammatory cytokines with a slight secretion of Th2 cytokines. Besides, no IL-10 was observed in both groups, indicating that MINPs with lipid modification were absence of immunosuppression. In conclusion, this study addresses an important implication of the lipid type and Fe concentration on the immune stimulation of cells and supports the potential for further development of biomedical applications.
文章类型Article
WOS标题词Science & Technology ; Technology
DOI10.1039/c7bm00244k
收录类别SCI
语种英语
关键词[WOS]OXIDE NANOPARTICLES ; DENDRITIC CELLS ; IMMUNE CELLS ; IN-VITRO ; DELIVERY ; FUNCTIONALIZATION ; THERAPY ; VACCINE ; VIRUS ; SIZE
WOS研究方向Materials Science
WOS类目Materials Science, Biomaterials
项目资助者National High Technology Research and Development Program(2016YFA0200303) ; National Natural Science Foundation of China(51573188 ; "Strategic Priority Research Program" of the Chinese Academy of Sciences(XDA09030301-3) ; Beijing Municipal Science & Technology Commission(Z161100002616015) ; 31522023 ; 51373177 ; 51572228)
WOS记录号WOS:000406321700026
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/23153
专题生化工程国家重点实验室
作者单位1.Southwest Jiaotong Univ, Minist Educ, Sch Mat Sci & Engn, Key Lab Adv Technol Mat, Chengdu 610031, Sichuan, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Navy Gen Hosp People Liberat Army, Dept Obstet & Gynecol, Beijing 100048, Peoples R China
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Qiao, Chenmeng,Yang, Jun,Chen, Lei,et al. Intracellular accumulation and immunological responses of lipid modified magnetic iron nanoparticles in mouse antigen processing cells[J]. BIOMATERIALS SCIENCE,2017,5(8):1603-1611.
APA Qiao, Chenmeng,Yang, Jun,Chen, Lei,Weng, Jie,&Zhang, Xin.(2017).Intracellular accumulation and immunological responses of lipid modified magnetic iron nanoparticles in mouse antigen processing cells.BIOMATERIALS SCIENCE,5(8),1603-1611.
MLA Qiao, Chenmeng,et al."Intracellular accumulation and immunological responses of lipid modified magnetic iron nanoparticles in mouse antigen processing cells".BIOMATERIALS SCIENCE 5.8(2017):1603-1611.
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