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Aquatic micro-pollutants removal with a biocatalytic membrane prepared by metal chelating affinity membrane chromatography
Fan, Jinxin1,2; Luo, Jianquan1,2; Wan, Yinhua1,2
2017-11-01
发表期刊CHEMICAL ENGINEERING JOURNAL
ISSN1385-8947
卷号327期号:NOV页码:1011-1020
摘要

Biocatalytic membranes are promising to remove micro-pollutants in aqueous environment due to their mild and green operation condition. However, more efforts need to be devoted to improving their removal efficiency and stability. In this study, metal chelating affinity membrane chromatography (MCAMC) was used to construct a biocatalytic membrane by selectively capturing laccase from a crude fermentation broth. Metal ions had a significant effect on the activity of the immobilized laccase and copper ion was the best choice. A pH of 4.5 was selected for laccase adsorption and its loading seemed the same under flow rates from 0.5 to 10 mL min(-1) thanks to the inherent convective transport of membrane chromatography. The pH value and salt concentration in the storage buffer had an obvious effect on the stability of the immobilized laccase, and the prepared biocatalytic membrane retained 87% of initial activity after 20 days storage. When applying such membrane to micro-pollutant removal (taking bisphenol A (BPA) as an example), a high BPA removal efficiency (99.3%) could be obtained. The biocatalytic membranes could be operated at a high flux of 50 L m(-2) h(-1) without recycling the permeate into the feed, and its throughput and BPA removal rate were superior to the most results in the literature. However, BPA removal decline (from 99.6% to 56.6% after five cycles) occurred during the successive water treatment due to the membrane fouling caused by BPA polymerization products. Membrane regeneration could be achieved by simple elution-cleaning-reloading, and the laccase activity and BPA removal were fully recovered. (C) 2017 Elsevier B.V. All rights reserved.

关键词Biocatalytic Membrane Laccase Micro-pollutant Bisphenol a Metal Chelating Affinity Membrane Chromatography
文章类型Article
WOS标题词Science & Technology ; Technology
DOI10.1016/j.cej.2017.06.172
收录类别SCI
语种英语
关键词[WOS]BISPHENOL-A REMOVAL ; REVERSIBLE IMMOBILIZATION ; LACCASE IMMOBILIZATION ; NANOFIBROUS MEMBRANE ; PROTEIN ADSORPTION ; PERFORMANCE ; NANOPARTICLES ; PURIFICATION ; CARBON ; DEGRADATION
WOS研究方向Engineering
WOS类目Engineering, Environmental ; Engineering, Chemical
项目资助者National Natural Science Foundation of China(21506229) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017069) ; "100 Talents Program" of Chinese Academy of Sciences
WOS记录号WOS:000408663800104
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/23180
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Fan, Jinxin,Luo, Jianquan,Wan, Yinhua. Aquatic micro-pollutants removal with a biocatalytic membrane prepared by metal chelating affinity membrane chromatography[J]. CHEMICAL ENGINEERING JOURNAL,2017,327(NOV):1011-1020.
APA Fan, Jinxin,Luo, Jianquan,&Wan, Yinhua.(2017).Aquatic micro-pollutants removal with a biocatalytic membrane prepared by metal chelating affinity membrane chromatography.CHEMICAL ENGINEERING JOURNAL,327(NOV),1011-1020.
MLA Fan, Jinxin,et al."Aquatic micro-pollutants removal with a biocatalytic membrane prepared by metal chelating affinity membrane chromatography".CHEMICAL ENGINEERING JOURNAL 327.NOV(2017):1011-1020.
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