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Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis
Chen, Huayou1,2; Ullah, Jawad1; Jia, Jinru1
2017
Source PublicationJOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY
ISSN1464-1801
Volume27Issue:3Pages:159-167
AbstractSpore surface display is the most desirable with enhanced effects, low cost, less time consuming and the most promising technology for environmental, medical, and industrial development. Spores have various applications in industry due to their ability to survive in harsh industrial processes including heat resistance, alkaline tolerance, chemical tolerance, easy recovery, and reusability. Yeast and bacteria, including gram-positive and -negative, are the most frequently used organisms for the display of various proteins (eukaryotic and prokaryotic), but unlike spores, they can rupture easily due to nutritive properties, susceptibility to heat, pH, and chemicals. Hence, spores are the best choice to avoid these problems, and they have various applications over nonspore formers due to amenability for laboratory purposes. Various strains of Clostridium and Bacillus are spore formers, but the most suitable choice for display is Bacillus subtilis because, according to the WHO, it is safe to humans and considered as "GRAS" (generally recognized as safe). This review focuses on the application of spore surface display towards industries, vaccine development, the environment, and peptide library construction, with cell surface display for enhanced protein expression and high enzymatic activity. Different vectors, coat proteins, and statistical analyses can be used for linker selection to obtain greater expression and high activity of the displayed protein. (C) 2017 S. Karger AG, Basel
KeywordBacillus Subtilis Spore Bioremediation Biocatalysis Surface Display Vaccine Development
SubtypeReview
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1159/000475177
Indexed BySCI
Language英语
WOS KeywordCOTB ANCHOR PROTEIN ; CELL-SURFACE ; FUSION PARTNER ; SYSTEM ; COAT ; EXPRESSION ; BACTERIUM ; ANTIGENS ; ENZYMES ; CARRIER
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
Funding OrganizationNational Key Basic Research Program of China (973 Program)(2011CBA00800) ; National Key Laboratory of Biochemical Engineering ; Key Agriculture Support Project of Jiangsu Province, PR China(BE2013400)
WOS IDWOS:000409331400003
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/23207
Collection生化工程国家重点实验室
Affiliation1.Jiangsu Univ, Inst Life Sci, Zhenjiang 21200, Jiangsu, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Chen, Huayou,Ullah, Jawad,Jia, Jinru. Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis[J]. JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY,2017,27(3):159-167.
APA Chen, Huayou,Ullah, Jawad,&Jia, Jinru.(2017).Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis.JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY,27(3),159-167.
MLA Chen, Huayou,et al."Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis".JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY 27.3(2017):159-167.
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