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The Positive Correlation of the Enhanced Immune Response to PCV2 Subunit Vaccine by Conjugation of Chitosan Oligosaccharide with the Deacetylation Degree
Zhang, Guiqiang1,2,3; Cheng, Gong2,3; Jia, Peiyuan2,3; Jiao, Siming2,3; Feng, Cui2,3; Hu, Tao2,3; Liu, Hongtao2,3; Du, Yuguang2,3
2017-08-01
Source PublicationMARINE DRUGS
ISSN1660-3397
Volume15Issue:8
Abstract

Chitosan oligosaccharides (COS), the degraded products of chitosan, have been demonstrated to have versatile biological functions. In primary studies, it has displayed significant adjuvant effects when mixed with other vaccines. In this study, chitosan oligosaccharides with different deacetylation degrees were prepared and conjugated to porcine circovirus type 2 (PCV2) subunit vaccine to enhance its immunogenicity. The vaccine conjugates were designed by the covalent linkage of COSs to PCV2 molecules and administered to BALB/c mice three times at two-week intervals. The results indicate that, as compared to the PCV2 group, COS-PCV2 conjugates remarkably enhanced both humoral and cellular immunity against PCV2 by promoting lymphocyte proliferation and initiating a mixed T-helper 1 (Th1)/T-helper 2 (Th2) response, including raised levels of PCV2-specific antibodies and an increased production of inflammatory cytokines. Noticeably, with the increasing deacetylation degree, the stronger immune responses to PCV2 were observed in the groups with COS-PCV2 vaccination. In comparison with NACOS (chitin oligosaccharides)-PCV2 and LCOS (chitosan oligosaccharides with low deacetylation degree)-PCV2, HCOS (chitosan oligosaccharides with high deacetylation degree)-PCV2 showed the highest adjuvant effect, even comparable to that of PCV2/ISA206 (a commercialized adjuvant) group. In summary, COS conjugation might be a viable strategy to enhance the immune response to PCV2 subunit vaccine, and the adjuvant effect was positively correlated with the deacetylation degree of COS.

KeywordChitosan Oligosaccharide Deacetylation Degree Adjuvant Porcine Circovirus Type 2 (Pcv2) Conjugation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.3390/md15080236
Indexed BySCI
Language英语
WOS KeywordPorcine Circovirus Type-2 ; Dendritic Cells ; Chitooligosaccharides ; Adjuvants ; Immunogenicity ; Nanoparticles ; Typhimurium ; Acetylation ; Infection ; Efficacy
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal
Funding OrganizationNational Programs for High Technology Research and Development (863 Programs)(2014AA093604) ; National Natural Science Fund, China(31500747 ; 31570801 ; U160820020)
WOS IDWOS:000408763400002
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/23209
Collection生化工程国家重点实验室
Affiliation1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Biopharmaceut Prod & Formulat Engn, PLA, Beijing 100190, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Guiqiang,Cheng, Gong,Jia, Peiyuan,et al. The Positive Correlation of the Enhanced Immune Response to PCV2 Subunit Vaccine by Conjugation of Chitosan Oligosaccharide with the Deacetylation Degree[J]. MARINE DRUGS,2017,15(8).
APA Zhang, Guiqiang.,Cheng, Gong.,Jia, Peiyuan.,Jiao, Siming.,Feng, Cui.,...&Du, Yuguang.(2017).The Positive Correlation of the Enhanced Immune Response to PCV2 Subunit Vaccine by Conjugation of Chitosan Oligosaccharide with the Deacetylation Degree.MARINE DRUGS,15(8).
MLA Zhang, Guiqiang,et al."The Positive Correlation of the Enhanced Immune Response to PCV2 Subunit Vaccine by Conjugation of Chitosan Oligosaccharide with the Deacetylation Degree".MARINE DRUGS 15.8(2017).
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