Knowledge Management System Of Institute of process engineering,CAS
The inhibition of LPS-induced inflammation in RAW264.7 macrophages via the PI3K/Akt pathway by highly N-acetylated chitooligosaccharide | |
Xu, Qingsong1,2; Liu, Meisi1; Liu, Qishun1; Wang, Wenxia1; Du, Yuguang3; Yin, Heng1 | |
2017-10-15 | |
Source Publication | CARBOHYDRATE POLYMERS
![]() |
ISSN | 0144-8617 |
Volume | 174Issue:OCTPages:1138-1143 |
Abstract | Chitooligosaccharide (COS) has been shown to regulate many biological functions, such as antimicrobial effect and antitumor activity. In the present study, highly N-acetylated chitooligosaccharide(NACOS) was prepared by N-acetylation of COS, and the anti-inflammatory activity of NACOS in macrophages were evaluated. The results indicated NACOS significantly suppressed the LPS-induced proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-alpha(TNF-alpha) expression. Furthermore, the increased levels of reactive oxygen species (ROS) and nitric oxide (NO) were repressed by NACOS in a dose dependent manner. However, NACOS itself had no significant effect on the cell viability and cellular morphology. Signal transduction studies demonstrated that NACOS remarkably inhibited LPS-enhanced phosphorylation of phosphatidylinositol 3-kinase (PI3K) and Akt. These findings provide a possible molecular mechanism by which NACOS inhibit LPS-induced inflammatory response in macrophages, and a basis for utilizing NACOS in pharmaceutical therapy against inflammation. (C) 2017 Elsevier Ltd. All rights reserved. |
Keyword | N-acetylated Chitooligosaccharide Lipopolysaccharides Interleukin-6 Tumor Necrosis Factor-alpha Inflammation Pi3k/akt Pathway |
Subtype | Article |
WOS Headings | Science & Technology ; Physical Sciences |
DOI | 10.1016/j.carbpol.2017.07.051 |
Indexed By | SCI |
Language | 英语 |
WOS Keyword | RAW 264.7 CELLS ; CHITOSAN OLIGOSACCHARIDES ; IN-VITRO ; ENDOTHELIAL-CELLS ; MOLECULAR-WEIGHT ; CHITIN ; EXPRESSION ; MICROGLIA ; DISEASES ; VIVO |
WOS Research Area | Chemistry ; Polymer Science |
WOS Subject | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
Funding Organization | Ocean Public Welfare Scientific Research Project, State Oceanic Administration of China(201405003) ; National High Technology Research and Development Program of China (863 Program)(2014AA093604) |
WOS ID | WOS:000407696800119 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ipe.ac.cn/handle/122111/23219 |
Collection | 研究所(批量导入) |
Affiliation | 1.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China |
Recommended Citation GB/T 7714 | Xu, Qingsong,Liu, Meisi,Liu, Qishun,et al. The inhibition of LPS-induced inflammation in RAW264.7 macrophages via the PI3K/Akt pathway by highly N-acetylated chitooligosaccharide[J]. CARBOHYDRATE POLYMERS,2017,174(OCT):1138-1143. |
APA | Xu, Qingsong,Liu, Meisi,Liu, Qishun,Wang, Wenxia,Du, Yuguang,&Yin, Heng.(2017).The inhibition of LPS-induced inflammation in RAW264.7 macrophages via the PI3K/Akt pathway by highly N-acetylated chitooligosaccharide.CARBOHYDRATE POLYMERS,174(OCT),1138-1143. |
MLA | Xu, Qingsong,et al."The inhibition of LPS-induced inflammation in RAW264.7 macrophages via the PI3K/Akt pathway by highly N-acetylated chitooligosaccharide".CARBOHYDRATE POLYMERS 174.OCT(2017):1138-1143. |
Files in This Item: | ||||||
File Name/Size | DocType | Version | Access | License | ||
The inhibition of LP(1529KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | Application Full Text |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment